Abstract: Objective To clone the apolipoprotein E gene Extron 4 of the Mongolian gerbil. Methods The primers was designed according to the published sequences in GenBank. A RT-PCR method was applied to clone the apolipoprotein E Extron 4 in liver of the Mongolian gerbil. The PCR products was sequenced and blast with related sequences. Results The results of PCR and sequence presented that the sequence of the apolipoprotein E gene Extron 4 in the Mongolian gerbil was a 987 bp sequences. The result of blast showed the identification between Mongolian gerbil and rat or mouse is 73%~74%, between Mongolian gerbil and human is 62%, between Mongolian gerbil and pig or bovine is above 60%, between Mongolian gerbil and no-human primates is 57%. The coding protein is 263 amino acid residuals. Conclusions The RT-PCR products was the sequence of the apolipoprotein E4 gene of Mongolian gerbil.

Key words: Apolipoprotein E Extron 4, cloning, Mongolian gerbil, Hyperlipidemia