Establishment and Preliminary Application of ELISA in Detecting Lymphocytic Choriomeningitis Virus Antibody in Mongolian Gerbil

doi:10.3969/j.issn.1674-5817.2015.06.009

Abstract

Abstract: Objectives To develope the ELISA method for determination of lymphocytic choriomeningitis virus (LCMV) antibody in Mongolian gerbils. Methods Raised the Vero cell, vaccinated the LCMV, prepared the normal Vero antigen and specific LCMV antigen, titrated the best working density of enzyme union, the normal and specific antigen and verified the specificity, sensitivity, accuracy and stability of the method. Result The best working density of normal, specific antigen and the enzyme union were 0.4 mg/mL, 10 mg/mL and 1∶5 000, respectively. The inter-assay coefficient of variation of normal antigen and specific antigen were 6.8% and 8.9%, respectively, the intra-assay average coefficient of variation were 9.3% and 8.4%, respectively. The detection sensitivity was 1∶1 280. There was no cross-reactivity with mammalian orthoreovirus 3 (Reo3) and murine encephalomyelitis virus (M-TMEV). The stability test shows the relative deviation was below 25%. Conclusion The ELISA method is good in specificity, sensitivity, duplication, and stability, so ELISA could be used in detecting the LCMV antibody.

Key words: Lymphocytic choriomeningitis virus (LCMV), ELISA, Mongolian gerbil

CLC Number:

Q95-33

WANG Ji, WEI Li, FU Rui, LI Xiao-bo, WANG Shu-jing, XING Jin, FENG Yu-fang, GONG Wei, YUE Bing-fei, HE Zheng-ming. Establishment and Preliminary Application of ELISA in Detecting Lymphocytic Choriomeningitis Virus Antibody in Mongolian Gerbil[J]. Laboratory Animal and Comparative Medicine, 2015, 35(6): 473-477.

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