Detection of Whole Methylation Level of Liver Genomic DNA in Mongolian Gerbil Based on Indirect ELISA

doi:10.3969/j.issn.1674-5817.2018.01.008

Abstract

Abstract: Objective To detect the genomic DNA methylation level in the liver of Mongolian gerbil by indirect ELISA.Methods Six newborn gerbils (newborn group),12 young gerbils (6 in young control group and 6 in young nonalcoholic fatty liver disease (NAFLD) model group,6 gerbils in the 8-month-old (middle and aged group) were employed,all were male.The serum samples (except newborn group) were collected for detection of the level of total cholesterol(TC),triglycerides(TG),high-density lipoprotein (HDL),low-density lipoprotein(LDL).At the same time,two samples of liver were collected for HE staining and detecting the whole methylation level of liver genomic DNA [ie,5-methylcytosine (5-mC) contentby indirect ELISA kit.Results The levels of serum TC and TG in the young model group and the middle and aged group were significantly difference with those in the young control group (P<0.05).Morphological examination revealed that the young model group and middle and aged groups developed obvious hepatic steatosis.There are a large number of piles of mononuclear cells and piles of erythrocytes in neonatal gerbil liver sinus.Overall methylation test results showed that high fat diet induced model young group gerbils>newborn gerbils>middle and aged gerbils>young control group gerbils.Conclusions The degree of fatty degeneration caused by NAFLD and age in Mongolian gerbil correlated with methylation level of genomic DNA.The method of indirect ELISA for detecting methylation level of liver genome DNA was simple,rapid and economic,which would be developed as a method to evaluate epigenetics of gerbils.

Key words: Mongolian gerbil, Genomic DNA whole methylation level, 5-methylcytosine(5-mC), Indirect ELISA, Nonalcoholic fatty liver disease (NAFLD)

CLC Number:

Q95-33

WANG Zhi-yuan, LIU Yue-huan. Detection of Whole Methylation Level of Liver Genomic DNA in Mongolian Gerbil Based on Indirect ELISA[J]. Laboratory Animal and Comparative Medicine, 2018, 38(1): 44-47.

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