Abstract: Objective To explore effects of PASG knockout on brain development and lay a foundation for research on molecular etiology of glioma. Methods No. 10-12 exons of murine PASG gene were selectively deleted with classical knockout method. Histological analysis and western blot assay were employed to examine CNS cell morphology and gene expression in mutant mice. Results PASG mutant mice displayed functional abnormality in multi-organs （brain, lung, kidney, bone and so on）, especially neurological development retardation, histological changes in hippocampal region and expression changes of related genes. Conclusions PASG is essential for regulating embryo development and cell proliferation. The disruption of PASG caused abnormality structurally and functionally in organs of mice. Moreover, the close relationship between neural stem cells and cells in hippocampus provided a new access to molecular mechanism of glioma formation.