Abstract: Objective To investigate the impact on the biochemical indexes of SD rats through different anticoagulants for blood sample treatment. Methods Twenty-one SD rats were used for this study. Blood sample was collected from the abdominal aorta and isolated to obtain serum as control, and plasm samples with EDTA-K2 anticoagulant, heparin sodium anticoagulant, and sodium citrate anticoagulant. Twelve biochemical indexes were measured for all the samples by automatic biochemical analyzer. Statistical analysis were performed. Results Compared with control serum, creatinine (Cre), aspartate amino transferase (AST), albumin (Alb) in sodium citrate anticoagulant plasma were significantly lower (P<0.05), lactate dehydrogenase (LDH) was increased significantly (P<0.05), and AST, LDH, alkaline phosphatase (ALP) in EDTA-K2 anticoagulant blood plasma were significantly lower (P<0.05), Alb was increased significantly (P<0.05), and total cholesterol (CHO), urea nitrogen (UREA), Cre, AST, creatine kinase (CK), LDH, ALP, total protein (TP), and Alb were significantly decreased after treatment with sodium citrate (P<0.05). There were differences in some biochemical indexes among the three anticoagulant plasmas. Conclusion In the preclinical study, different anticoagulants have a profound influence on their biochemical indexes. Therefore, only partial biochemical indexes can be determined by using heparin sodium anticoagulant plasma and EDTA anticoagulant plasma instead of serum.

Key words: Anticoagulant, Serum, Plasma, Biochemical index, Rat