Abstract: Objective To explore a reliable and stable method for establishing hepatic fibrosis model in mice through different dosing level of carbon tetrachloride (CCl₄).Methods Sixty male C57BL/6J mice were randomly divided into control group and model group.The mice in model groups were intraperitoneally injected with 10% CCl₄ at the dosage of 5 mL/kg and 20% CCl₄ at the dosage of 1mL/kg respectively for 8 weeks,twice per week.Mice were sacrificed at the 2nd,4th,6th,8th week after injection.The degree of hepatic fibrosis was evaluated by HE and Masson staining.The changes of alanine aminotransferase (ALT),aspartate aminotransferase (AST) were detected in serum.The oxidative index malondialdehyde (MDA) and antioxidant indices,glutathione (GSH),superoxide dismutase (SOD) were detected in liver homogenate.The protein levels of collagen I and collagen III in liver tissues were determined by Western blotting.Results HE and Masson staining showed that the degree of hepatic fibrosis was aggravated as the development of the model.The expression of collagen was gradually increased,the serum levels of ALT,AST and MDA in liver tissues were significantly increased,while the activities of SOD and GSH were significantly decreased.At the same point of model progress,the degree of hepatic fibrosis of 10% CCl₄ (5 mL/kg) group was more severe than that of 20% (1 mL/kg) group.The levels of ALT,AST and MDA in 10% CCl₄ (5 mL/kg) were significantly higher than 20% CCl₄ (1 mL/kg) group.In addition,the SOD activity and GSH were lower than those of 20% (1 mL/kg) group.Conclusion Both of two methods can induce hepatic fibrosis model,and 10% CCl₄ (5 mL/kg) has advantages such as better effect and shorter modeling time compared with 20% CCl₄ (1 mL/kg),therefor,it is an ideal method for establishment of liver fibrosis model in mice.

Key words: Hepatic fibrosis, Carbon tetrachloride (CCl₄), Mice, Model