Comparative Medical Significance of Mechanism on Radiation Induced Heart Disease in Rats

doi:10.3969/j.issn.1674-5817.2018.03.005

Abstract

Abstract: Objective To investigate the model establishment about radiation induced heart (RIHD) in rat and observed the changes of echocardiography,electrocardiogram and serum biomarkers at different time points.Methods Ninety clean grade male SD rats were divided into normal control group and irradiation group randomly,the irradiation group was divided into four groups different dosage which 5Gy,10 Gy,20 Gy and 30 Gy,18 rats in each group,irradiate the heart with different dosage through varian linear accelerator,the rats were killed after irradiation on 2,4 and 12 weekend respectively.Weight of rat before euthanized,left ventricular ejection fraction (LVEF),left venteicular systolic pressuer (LVSP),left ventricular end diastolic pressure (LVEDP),electrocardiogram(ECG),serum creatine kinase isoenzyme-MB (CKMB),cardiactroponin Ⅰ (cTnI) and weight of whole heart are all detected with different tools.Results Compared with normal control group,with the irradiation dose increased,LVEF and LVSP decreased gradually in irradiation group.LVEDP rise gradually (P<0.05 or P<0.01).When the increase of irradiation dose,the heart rate increased and the ST segment elevated significantly (P<0.05 or P<0.01).Serum CKMB and CTnI increased in different degrees (P<0.05 or P<0.01),reach statistical significance.there were no significant differences in all the indenes between 30 Gy group and 20 Gy group (P>0.05),no statistical significance.Conclusions Radiation induced heart injury model was established on rat after four weeks irradiation at 22 Gy dosage.

Key words: Rat, Radiation induced heart disease (RIHD), Animal model, Echocardiography, Electrocardiogram, Serum marker

CLC Number:

Q95-33

MA Jin-ke, GAO Shi-le, HU Zong-tao, WANG Chong, GAO Shan, DONG Liu-yi, LIU Yang, TANG Zheng-zhong. Comparative Medical Significance of Mechanism on Radiation Induced Heart Disease in Rats[J]. Laboratory Animal and Comparative Medicine, 2018, 38(3): 188-193.

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