Effects of Mifepristone on Expression of Integrin αvβ3 and Vascular Endothelial Growth Factor in Wistar Rats at Implantation Window Phase

doi:10.3969/j.issn.1674-5817.2017.05.011

Abstract

Abstract: Objective To investigate the effect of mifepristone on the expression of integrin α_vβ₃and vascular endothelial growth factor (VEGF) gene, and the effect on ultrastructure of endometrium at implantation window phase in Wistar rats. Methods Thirty-six Wistar rats were divided to four groups, the rats of two experimental groups were orally administered with 5 mg/kg, 10 mg/kg of mifepristone respectively one day after conception. The endometrium was collected 4.5 days after mate both in two experimental groups and two control groups. Ultrastructure of the endometrium were observed by transmission electron microscope, and the expression of integrin α_vβ₃ and VEGF gene in endometrial were tested by RT-PCR method, meantime, the contents of estrogen and progesterone in serum were detected by ELISA technique. Results Mifepristone can inhibits the expression of integrin α_vβ₃, VEGF and progesterone, and increase the content of estrogen (P>0.05). The down-regulation of the three factors and the changes of the ultrastructure of endometrium has obvious dose-response relationship with mifepristone. Conclusion The expression of integrin α_vβ₃ and VEGF gene were decreased differently by mifepristone as well as the content of progesterone, eventually it will affect the ultrastructure of endometrium.

Key words: Mifepristone, Integrin α_vβ₃, Vascular endothelial growth factor (VEGF), Estrogen

CLC Number:

Q95-33

TANG Bin, LI Fu-rong, FU Xing-Lu, JIANG Guo-Jian, ZHAO Yong, ZHANG Wei. Effects of Mifepristone on Expression of Integrin α_vβ₃ and Vascular Endothelial Growth Factor in Wistar Rats at Implantation Window Phase[J]. Laboratory Animal and Comparative Medicine, 2017, 37(5): 399-404.

References

[1] Kim M, Carman CV, Springer TA.Biodirectional trans-memnbrane signaling by cytoplasmic domain separation in integrins[J]. Science, 2003, 301(5640):1720-1725.
[2] Bauer SM, Bauer RJ, Velazquez OC, et al.Angiogenesis, vasculogenesis, and induction of healing in chronic wounds[J]. Vasc Endovascu-lar Surg, 2005, 4:293-306.
[3] Carano RA, Filvaroff EH.Angiogenesis and bone repair[J]. Drug Discov Today, 2003, 21:980-989.
[4] Oxboel J, Binderup T, Knigge U, et al.Quantitative gene- expression of the tumor angiogenesis markers vascular endo thelial growth factor, integrin alphaV and integrin beta3 in human neuroendocrine tumors[J]. Oncol Rep, 2009, 3:769-775.
[5] 王成伟, 庞琦, 张珑, 等. 人脑胶质瘤中微血管密度和血管内皮生长因子表达的意义[J]. 癌症, 2004, 3:326-327.
[6] Senger DR, Gallis J, Dvorak AM, et al.Tumor cells secrete a vascular permeability factor that promote accumulation of ascites fluid[J]. Science, 1983, 219(4587):983-985.
[7] 刘桂君, 陈国江, 黎燕, 等. 血管内皮生长因子在肿瘤生长转移中的作用机制研究进展[J]. 国际药学研究杂志, 2012,39(5):391-395.
[8] Hayashi A, Tanabe A, Kawabe S, et al.Dienogest increases the progesterone receptor isoform B/A ratio in patients with ovarian endometriosis[J]. J Ovarian res, 2012, 5(1):31-38
[9] 陆叶, 于丽, 陈春玲. 雌、孕激素和米非司酮对子宫内膜癌细胞的作用[J]. 北京大学学报, 2005, 37(5):284-286.
[10] Barrier BF.Immunology of endometriosis[J]. Clin Obstet Gynecol, 2010, 53(2):397-402.
[11] Lessey BA, Lebovic DI, Taylor RN.Eutopic endometrium in women with endometriosis: ground zero for the study of implantation defects[J]. Semin Reprod Med, 2013, 31(2):109-124.
[12] Zachary I.VEGF signalling: integration and multi-tasking in endothelial cell biology[J]. Biochem Soc Trans, 2013, 31(1): 1171-1177.
[13] 赵轩, 吴华真, 王宝金, 等. 米非司酮对子宫内膜异位症患者在位及异位子宫内膜中血管内皮生长因子表达及微血管密度的影响[J]. 中华妇产科杂志, 2005, 40(4):277-278.
[14] Gangula PR, SuPowit SC, Wimalawansa SJ, et al. Caleitonin gene-relatedEPtide in a depressor in N(G)-vitro-L-arginine methylester induced hypertension during preaney[J]. Hypertension, 2012, 29(lptZ):248-253.
[15] Reinseh RC, Murphy A, Morales AJ, et al.The effect of RU-486 and leuprolide acetate on uterine blood flow in fibroid uterus: a prospective Randomized study[J]. Am J Obstet Gynecol, 2014, 170(1):1623-1627.
[16] Serini G, Valdembri D, Bussolino F.Integrins and angiogenesis: asticky business[J]. Exp Cell Res, 2006, 312(5):651-658.
[17] Matou S, Colliec-Jouault S, Galy-Fauroux I, et al.Effect of anoversulfated exopolysaccharide on angiogenesis induced by fibroblastgrowth factor-2 or vascular endothelial growth factor in vitro[J].Biochem Phamacol, 2005, 9(5):751-759.
[18] Hosotani R, Kawaguchi M, Masui T, et al.Expression of integrinαvβ3 in pancreatic carcinoma: relation to MMP-2 activation andlymph node metastasis[J]. Pancreas, 2002, 25(2):e30-35.
[19] 谢杏美, 王玉霞. 米非司酮配伍米索前列醇治疗稽留流产和终止10~16周妊娠的效果比较[J]. 实用妇产科杂志, 2012, 28(8):693-695.
[20] 王婷. 米非司酮对子宫内膜作用的研究进展[J]. 现代妇产科进展, 2013, 12(4):295-297.
[21] 马成斌, 刘平, 曹美良. 盆腔子宫内膜异位症术后应用不同剂量米非司酮治疗的疗效观察[J]. 实用妇产科杂志, 2012, 23(1):61-63.
[22] 韩兆忠, 刘茜, 郑伟然. 桂枝茯苓胶囊联合米非司酮治疗子宫肌瘤临床疗效和安全性的系统性评价[J]. 中医药现代化, 2016, 18(3):543-551.
[23] 张恒柱, 武永康, 李耀华, 等. 米非司酮对人脑膜瘤治疗作用的实验研究[J]. 中华神经外科杂志, 2005, 21(1):51-54.
[24] 刘桂君, 陈国江, 黎燕. 血管内皮生长因子在肿瘤生长转移中的作用机制研究进展[J]. 国际药学研究杂志, 2012,39(5):391-395.
[25] 赵公平, 刘兴, 王梦溪. 米非司酮治疗多囊卯巢综合征的疗效观察(附临床病例96例)[J]. 中华妇科杂志, 2008, 10(3):22-23.

Effects of Mifepristone on Expression of Integrin α_vβ₃ and Vascular Endothelial Growth Factor in Wistar Rats at Implantation Window Phase

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