The Research and Application of Mammary Gland Epithelial Cells

doi:10.3969/j.issn.1674-5817.2013.03.016

Abstract

Abstract: Transgenic animal mammary gland bioreactor has been developed for the drugs production as one of the most promising bio-pharmaceutical methods in recent decades. The key technique in the process of the mammary gland bioreactors generation relies on the construction of exogenous gene vectors. It is crucial to establish a simple and quick method to test the accuracy and expression level of constructed gene vectors. As the growth progress and biophysiological lactation functions of mammary glands could be representatively investigated in mammary epithelial cells in vitro, mammary epithelial cells are becoming ideal cell models for detecting the efficacy of constructed-vectors in transgenic animals generations. In this review, it briefly introduced the structure of mammary gland tissue and characteristics of proliferation, differentiation, induction and maintenance of mammary epithelial cells. Additionally, it was summarized the culture approach of mammary epithelial cells and its application in biological and medical researches.

Key words: Mammary gland bioreactor, Gene vector, Gland epithelial cell, Model of in vitro detection

CLC Number:

Q95-33

YAN Ya-bin, HUANG Ying. The Research and Application of Mammary Gland Epithelial Cells[J]. Laboratory Animal and Comparative Medicine, 2013, 33(3): 237-242.

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