Expression of 5-HTR1A in Hypothalamus of Pre-Menstrual Syndrome Liver-qi Depression Model Rats

doi:10.3969/j.issn.1674-5817.2012.04.004

Abstract

Abstract: Objective To investigate the pathogenesis and drug target through the study about expression of 5-HTR1A in the hypothalamus of Pre-Menstrual Syndrome (PMS) Liver-qi Depression Model Rats. Methods The PMS Liver-qi Depression model was established by bounding the rat's feet, Then it was divided into control group, model group and treated group which were treated with Jingqianshu pellet. The mRNA and protein expressions of 5-HTR1A in the hypothalamus of the model Rats were detected by RT-PCR and Western Blotting separately. The data were analyzed by the one-way ANOVA and LSD with the software SPSS 16.0. Results The mRNA and protein expressions of 5-HTR1A in the hypothalamus of model group were decreased significantly compared with the control group. And the expressions of the treated group with Jingqianshu pellet were increased significantly compared with the model group. But there were no significant difference between the treated and the control groups. Conclusion The expressions of 5-HTR1A in the hypothalamus of PMS Liver-qi Depression Model Rats were decreased significantly compared with the control rats, and then the expressions level could recovered to the control level after treated with Jingqianshu pellet. Therefore, it was demonstrated that the expression of 5-HTR1A and PMS Liver-qi Depression may related, and the gene could be the drug target of Jingqianshu granule.

Key words: 5-HTR1A, PMS Liver-qi Depression, Rat

CLC Number:

Q95-33

XU Kai-yong, GAO Jie, SONG Chun-hong, LI Zi-fa, WEI Sheng, ZHANG Hui-yun. Expression of 5-HTR1A in Hypothalamus of Pre-Menstrual Syndrome Liver-qi Depression Model Rats[J]. Laboratory Animal and Comparative Medicine, 2012, 32(4): 274-278.

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