Genetic Mornitoring for Inbred Mice with Single Nucleotide Polymorphisms

doi:10.3969/j.issn.1674-5817.2018.01.003

Abstract

Abstract: Objective To detect the genetic quality of 29 inbred strains mice from 36 different colonies using 95 single nucleotide polymorphism (SNP) loci and analyze the different genotype among different strains.Methods All genotypes of samples from domestic including 36 inbred mice colonies such as C57BL/6J,BALB/c were identified by the methods of matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) using 95 SNPs referenced from domestic and international literature.Then the numbers of loci with different genotypes between each two strains were analyzed and compared.Results Totally 29 colonies (80.56%) of the mouse were successfully genotyped with 95 SNPs while very few strains weren’t,because of sample’s quality focusing on 3 strains with the background of BALB/c.Most loci were homozygous and the rate of monomorphic loci within strains was up to 98.95%.The numbers of loci with different genotypes between each two colonies reached maximal 58 loci and minimal 1 locus which distributed in the same strain from distinct sources and the gene modified lines between their backgrounds.Conclusions The selected SNPs could be applied to mornitor and identify the genetic condition of inbred mice.However,SNP loci panel should be optimized on behalf of each strain’s characteristics.

Key words: Inbred mice, Single nucleotide polymorphism (SNP), Matrix assisted laser desorption

CLC Number:

Q95-33

LI Yin-yin, CHEN Zhen-wen, LIU Li-jun, LI Chang-long, LIU Xin, LV Jian-yi, GUO Meng, DU Xiao-yan. Genetic Mornitoring for Inbred Mice with Single Nucleotide Polymorphisms[J]. Laboratory Animal and Comparative Medicine, 2018, 38(1): 16-21.

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