Laboratory Animal and Comparative Medicine ›› 2016, Vol. 36 ›› Issue (1): 24-31.DOI: 10.3969/j.issn.1674-5817.2016.01.005

Previous Articles     Next Articles

Application of Single Nucleotide Polymorphism Genotyping Panel in Genetic Monitoring of Laboratory Mice

ZHAO Li-ya1, ZHANG Rong1, ZHAO Ying1, XING Zheng-hong1, CHEN Guo-qiang1   

  1. 1. Sino-British SIPPR/BK Laboratory Animal Co., Ltd., Shanghai 201203, China;
    2. Shanghai Laboratory Animal Research Center, Shanghai 201203, China
  • Received:2015-06-29 Online:2016-02-25 Published:2016-02-25

Abstract: Objective To develop multiple PCR-LDR genotyping protocols for monitoring genetic contamination in inbreed mice in a fast and reliable way. Method In total 51 single nucleotide polymorphisms (SNPs) from public SNP database were chosen and divided into five groups. These 51 SNPs are wildly distributed and there are least one SNP on each autosomal chromosome and the sex chromosome. A multiple PCR-LDR genotyping protocol was established as a genetic quality control approach to monitor the genotypes of 7 inbred mice. Results The homozygosity is 100% for the 7 tested mice. The genetic background of the inbred mice from the same source is identical. For the same inbred mice from different companies, several SNPs are different. The SNPs are different at a9、a10、b5、b9、c1、c12、e1、e2 for CBA/Ca/Bkl and CBA/JSlac, while the SNPs are different at c7, c10 for C57BL/6/BKl and C57BL/6JSlac. However, there are five loci which are the same in the 7 inbred mice of different companies. So there are 46 effective loci which are different in the 7 inbred mice of different companies. Conclusion The multiple PCR-LDR genotyping protocol is used to monitor the genetic differences of inbred mice from two companies. The results of this study provide a rapid and high-throughput genotyping approach, which is sufficient for genetic contamination monitoring and strain identification.

Key words: Genetic quality monitoring, Inbred mice, Single Nucleotide Polymorphisms (SNP), multiple PCR-LDR, Genotyping protocol

CLC Number: