Explanation and Elaboration for the ARRIVE Guidelines 2.0—Reporting Animal Research and In Vivo Experiments (Ⅲ)

doi:10.12300/j.issn.1674-5817.2023.039

Abstract

Abstract:

Improving the reproducibility of biomedical research results is a major challenge.Researchers reporting their research process transparently and accurately can help readers evaluate the reliability of the research results and further explore the experiment by repeating it or building upon its findings. The ARRIVE 2.0 guidelines, released in 2019 by the UK National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs), provide a checklist applicable to any in vivo animal research report. These guidelines aim to improve the standardization of experimental design, implementation, and reporting, as well as the reliability, repeatability, and clinical translatability of animal experimental results. The use of ARRIVE 2.0 guidelines not only enriches the details of animal experimental research reports, ensuring that information on animal experimental results is fully evaluated and utilized, but also enables readers to understand the content expressed by the author accurately and clearly, promoting the transparency and integrity of the fundamental research review process. At present, the ARRIVE 2.0 guidelines have been widely adopted by international biomedical journals. This article is a Chinese translation based on the best practices of international journals following the ARRIVE 2.0 guidelines in international journals, specifically for the complete interpretation of the ARRIVE 2.0 guidelines published in the PLoS Biology journal in 2020 (original text can be found at https://arriveguidelines.org). The third part of the article includes the items 8-10 of ARRIVE 2.0 Essential 10, which covers "experimental animals" "experimental procedures" and "results". Its aim is to promote the full understanding and use of the ARRIVE 2.0 guidelines by domestic researchers, enhance the standardization of experimental animal research and reporting, and promote the high-quality development of experimental animal technology and comparative medicine research in China.

Key words: Animal experiment, ARRIVE 2.0 guidelines, ARRIVE essential 10, Experimental animals, Experimental procedures, Experimental results

CLC Number:

R-332

Xiaoyu LIU, Xuancheng LU, Xiaomeng SHI, Yuzhou ZHANG, Chao LÜ, Guoyuan CHEN, Xiao LU, Yu BAI, Jing GAO, Yao LI, Yonggang LIU, Yufeng TAO, Wanyong PANG. Explanation and Elaboration for the ARRIVE Guidelines 2.0—Reporting Animal Research and In Vivo Experiments (Ⅲ)[J]. Laboratory Animal and Comparative Medicine, 2023, 43(4): 446-456.

Figures/Tables 5

References 154

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编号 No.	实验流程 Procedures	实验资源 Resources
1	药理研究（包括干预和对照）	细胞系
	● 药物配方	● 鉴别
	● 剂量	● 来源
	● 体积	● 验证和认证
	● 浓度	● RRID^[128-129]
	● 给药部位和途径
	● 给药频率
	● 溶媒或载体溶液的配方和体积
	● 任何可以表明所使用的药剂到达靶组织的证据
2	外科手术（包括假手术）	试剂（如抗体、化学品）
	● 手术过程描述	● 制造商
	● 使用的麻醉剂（包括上文药理研究部分所列剂量及信息）	● 供应商
	● 术前和术后镇痛方案	● 目录号
	● 术前程序（如禁食）	● 批号（如适用）
	● 无菌术	● 药物纯度（如适用）
	● 监测（如评估手术麻醉状况）	● RRID
	● 流程是否是终末性质的
	● 术后程序
	● 手术持续时间和麻醉持续时间
	● 测量的身体指标
3	病原体感染（包括干预和对照）	设备和软件
	● 传染性病原体	● 制造商
	● 感染最高剂量	● 供应商
	● 溶媒或载体溶液的配方和体积	● 型号/版本号
	● 感染部位和途径	● 校准程序（如适用）
	● 感染时间或频率	● RRID
4	安乐死
	● 安乐死方法，包括该方法遵循的人道标准，如AVMA^[127]
	● 药剂（如使用）（包括上文药理研究部分所列剂量及信息）
	● 安乐死前或安乐死期间为减轻疼痛和痛苦而采取的任何措施
	● 安乐死的时间
	● 安乐死后收集的组织及其时间

编号 No.	实验流程 Procedures	实验资源 Resources
1	药理研究（包括干预和对照）	细胞系
	● 药物配方	● 鉴别
	● 剂量	● 来源
	● 体积	● 验证和认证
	● 浓度	● RRID^[128-129]
	● 给药部位和途径
	● 给药频率
	● 溶媒或载体溶液的配方和体积
	● 任何可以表明所使用的药剂到达靶组织的证据
2	外科手术（包括假手术）	试剂（如抗体、化学品）
	● 手术过程描述	● 制造商
	● 使用的麻醉剂（包括上文药理研究部分所列剂量及信息）	● 供应商
	● 术前和术后镇痛方案	● 目录号
	● 术前程序（如禁食）	● 批号（如适用）
	● 无菌术	● 药物纯度（如适用）
	● 监测（如评估手术麻醉状况）	● RRID
	● 流程是否是终末性质的
	● 术后程序
	● 手术持续时间和麻醉持续时间
	● 测量的身体指标
3	病原体感染（包括干预和对照）	设备和软件
	● 传染性病原体	● 制造商
	● 感染最高剂量	● 供应商
	● 溶媒或载体溶液的配方和体积	● 型号/版本号
	● 感染部位和途径	● 校准程序（如适用）
	● 感染时间或频率	● RRID
4	安乐死
	● 安乐死方法，包括该方法遵循的人道标准，如AVMA^[127]
	● 药剂（如使用）（包括上文药理研究部分所列剂量及信息）
	● 安乐死前或安乐死期间为减轻疼痛和痛苦而采取的任何措施
	● 安乐死的时间
	● 安乐死后收集的组织及其时间

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