Interpretation and Elaboration for the ARRIVE Guidelines 2.0—Animal Research: Reporting In Vivo Experiments (V)

doi:10.12300/j.issn.1674-5817.2023.146

Abstract

Abstract:

Improving the reproducibility of biomedical research results is a major challenge. Transparent and accurate reporting of the research process enables readers to evaluate the reliability of the research results and further explore the experiment by repeating it or building upon its findings. The ARRIVE 2.0 guidelines, released in 2019 by the UK National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs), provide a checklist that is applicable to any in vivo animal research report. These guidelines aim to improve the standardization of experimental design, implementation, and reporting, as well as enhance the reliability, repeatability, and clinical translation of animal experimental results. The use of the ARRIVE 2.0 guidelines not only enriches the details of animal experimental research reports, ensuring that information on animal experimental results is fully evaluated and utilized, but also enables readers to understand the content expressed by the author accurately and clearly, promoting the transparency and completeness of the fundamental research review process. At present, the ARRIVE 2.0 guidelines have been widely adopted by international biomedical journals. This article is based on the best practices following the ARRIVE 2.0 guidelines in international journals, and it interprets, explains, and elaborates in Chinese the fifth part of the comprehensive version of the ARRIVE 2.0 guidelines published in PLoS Biology in 2020 (the original text can be found at https://arriveguidelines.org). This section includes the items 6-11 of Recommended 11 section, covering "Animal Care and Monitoring", "Interpretation/Scientific Implications", "Generalisability/Translation", "Protocol Registration", "Data Access" and "Declaration of Interests". Its aim is to promote a comprehensive understanding and use of the ARRIVE 2.0 guidelines among domestic researchers, to enhance the standardization of experimental animal research and reporting, and to promote high-quality development of experimental animal sciences and comparative medicine research in China.

Key words: Animal experiment, ARRIVE 2.0 guidelines, ARRIVE Recommended set, Pain management, Animal care and monitoring

CLC Number:

R-332

Zhengwen MA, Xiaying LI, Xiaoyu LIU, Yao LI, Jian WANG, Jin LU, Guoyuan CHEN, Xiao LU, Yu BAI, Xuancheng LU, Yonggang LIU, Wanyong PANG, Yufeng TAO. Interpretation and Elaboration for the ARRIVE Guidelines 2.0—Animal Research: Reporting In Vivo Experiments (V)[J]. Laboratory Animal and Comparative Medicine, 2024, 44(1): 105-114.

Figures/Tables 1

References 276

5	KILKENNY C, BROWNE W J, CUTHILL I C, et al. Improving bioscience research reporting: the ARRIVE guidelines for reporting animal research[J]. PLoS Biol, 2010, 8(6): e1000412. DOI: 10.1371/journal.pbio.1000412 .
13	PERCIE DU SERT N, HURST V, AHLUWALIA A, et al. The ARRIVE guidelines 2.0: updated guidelines for reporting animal research[J]. PLoS Biol, 2020, 18(7): e3000410. DOI: 10.1371/journal.pbio.3000410 .
14	DU SERT N P, AHLUWALIA A, ALAM S, et al. Reporting animal research: explanation and elaboration for the ARRIVE guidelines 2.0[J]. PLoS Biol, 2020, 18(7): e3000411. DOI: 10.1371/journal.pbio.3000411 .
15	张俊彦, 刘晓宇, 李垚, 等. 动物实验研究报告的国际指南ARRIVE 2.0介绍及期刊实施计划[J]. 实验动物与比较医学, 2023, 43(1): 86-94. DOI: 10.12300/j.issn.1674-5817.2023.014 .
	ZHANG J Y, LIU X Y, LI Y, et al. Introduction to the international guide for animal research reporting ARRIVE 2.0, and its implementation plan in the journal[J]. Lab Anim Comp Med, 2023, 43(1): 86-94. DOI: 10.12300/j.issn.1674-5817.2023.014 .
103	王剑, 卢今, 马政文, 等. «动物研究: 体内实验报告»即ARRIVE 2.0指南的解释和阐述(一)[J]. 实验动物与比较医学, 2023, 43(2): 213-224. DOI: 10.12300/j.issn.1674-5817.2023.043 .
	WANG J, LU J, MA Z W, et al. Explanation and elaboration for the ARRIVE guidelines 2.0—reporting animal research and in vivo experiments(Ⅰ)[J]. Lab Anim Comp Med, 2023, 43(2): 213-224. DOI: 10.12300/j.issn.1674-5817.2023.043 .
104	陈国元, 卢晓, 白玉, 等. «动物研究: 体内实验报告»即ARRIVE 2.0指南的解释和阐述(二)[J]. 实验动物与比较医学, 2023, 43(3): 323-331. DOI: 10.12300/j.issn.1674-5817.2023.042 .
	CHEN G Y, LU X, BAI Y, et al. Explanation and elaboration of the ARRIVE guidelines 2.0—reporting animal research and in vivo experiments(Ⅱ)[J]. Lab Anim Comp Med, 2023, 43(3): 323-331. DOI: 10.12300/j.issn.1674-5817.2023.042 .
155	刘晓宇, 卢选成, 师晓萌, 等. «动物研究: 体内实验报告»即ARRIVE 2.0指南的解释与阐述(三)[J]. 实验动物与比较医学, 2023, 43(4): 446-456. DOI: 10.12300/j.issn.1674-5817.2023.039 .
	LIU X Y, LU X C, SHI X M, et al. Explanation and elaboration for the ARRIVE guidelines 2.0—reporting animal research and in vivo experiments(Ⅲ)[J]. Lab Anim Comp Med, 2023, 43(4): 446-456. DOI: 10.12300/j.issn.1674-5817.2023.039 .
219	李夏莹, 田永路, 刘晓宇, 等.«动物研究:体内实验报告»即ARRIVE 2.0指南的解释和阐述(四)[J]. 实验动物与比较医学, 2023, 43(6): 659-668. DOI: 10.12300/j.issn.1674-5817.2023.142 .
	LI X Y, TIAN Y L, LIU X Y, et al. Explanation and elaboration for the ARRIVE Guidelines 2.0—reporting animal research and in vivo experiments ( Ⅳ) [J]. Lab Anim Comp Med, 2023, 43(6): 659-668. DOI: 10.12300/j.issn.1674-5817.2023.142 .
220	JIRKOF P. Side effects of pain and analgesia in animal experimentation[J]. Lab Anim, 2017, 46(4):123-128. DOI: 10.1038/laban.1216 .
221	CARBONE L, AUSTIN J. Pain and laboratory animals: publication practices for better data reproducibility and better animal welfare[J]. PLoS One, 2016, 11(5): e0155001. DOI: 10.1371/journal.pone.0155001 .
222	GASPANI L, BIANCHI M, LIMIROLI E, et al. The analgesic drug tramadol prevents the effect of surgery on natural killer cell activity and metastatic colonization in rats[J]. J Neuroimmunol, 2002, 129(1-2):18-24. DOI: 10.1016/s0165-5728(02)00165-0 .
223	GETTY C M, DILGER R N. Moderate perinatal choline deficiency elicits altered physiology and metabolomic profiles in the piglet[J]. PLoS One, 2015, 10(7): e0133500. DOI: 10.1371/journal.pone.0133500 .
224	TEILMANN A C, FALKENBERG M K, HAU J, et al. Comparison of silicone and polyurethane catheters for the catheterization of small vessels in mice[J]. Lab Anim, 2014, 43(11):397-403. DOI: 10.1038/laban.570 .
225	BUSTAMANTE R, DAZA M A, CANFRÁN S, et al. Comparison of the postoperative analgesic effects of cimicoxib, buprenorphine and their combination in healthy dogs undergoing ovariohysterectomy[J]. Vet Anaesth Analg, 2018, 45(4):545-556. DOI: 10.1016/j.vaa.2018.01.003 .
226	MUHLHAUSLER B S, BLOOMFIELD F H, GILLMAN M W. Whole animal experiments should be more like human randomized controlled trials[J]. PLoS Biol, 2013, 11(2): e1001481. DOI: 10.1371/journal.pbio.1001481 .
227	BAUER G, DAO M A, CASE S S, et al. In vivo biosafety model to assess the risk of adverse events from retroviral and lentiviral vectors[J]. Mol Ther, 2008, 16(7):1308-1315. DOI: 10. 1038/mt.2008.93 .
228	BELLANGER S, BENREZZAK O, BATTISTA M C, et al. Experimental dog model for assessment of fasting and postprandial fatty acid metabolism: pitfalls and feasibility[J]. Lab Anim, 2015, 49(3):228-240. DOI: 10.1177/002367721456 6021 .
229	HENDRIKSEN C, MORTON D, CUSSLER K. Use of humane endpoints to minimise suffering[M]//The COST Manual of Laboratory Animal Care and Use. Boca Raton: CRC Press, 2016:333-353. DOI: 10.1201/b13591-14 .
230	HAWKINS P, MORTON D B, BURMAN O, et al. A guide to defining and implementing protocols for the welfare assessment of laboratory animals: eleventh report of the BVAAWF/FRAME/RSPCA/UFAW Joint Working Group on Refinement[J]. Lab Anim, 2011, 45(1):1-13. DOI: 10.1258/la. 2010.010031 .
231	WORKMAN P, ABOAGYE E O, BALKWILL F, et al. Guidelines for the welfare and use of animals in cancer research[J]. Br J Cancer, 2010, 102(11):1555-1577. DOI: 10.1038/sj.bjc.6605642 .
232	DU SERT N P, ALFIERI A, ALLAN S M, et al. The IMPROVE guidelines (ischaemia models: procedural refinements of in vivo experiments)[J]. J Cereb Blood Flow Metab, 2017, 37(11):3488-3517. DOI: 10.1177/0271678X17709185 .
233	MORTON D B. A systematic approach for establishing humane endpoints[J]. ILAR J, 2000, 41(2):80-86. DOI: 10.1093/ilar.41.2.80 .
234	MUSCELLA A, VETRUGNO C, COSSA L G, et al. In vitro and in vivo antitumor activity of[Pt(O, O'-acac)(γ-acac)(DMS)] in malignant pleural mesothelioma[J]. PLoS One, 2016, 11(11): e0165154. DOI: 10.1371/journal.pone.0165154 .
235	GLASZIOU P, ALTMAN D G, BOSSUYT P, et al. Reducing waste from incomplete or unusable reports of biomedical research[J]. Lancet, 2014, 383(9913):267-276. DOI: 10.1016/S0140-6736(13)62228-X .
236	SCHIRMER B, WEDEKIND D, GLAGE S, et al. Deletion of IL-18 expression ameliorates spontaneous kidney failure in MRLlpr mice[J]. PLoS One, 2015, 10(10): e0140173. DOI: 10.1371/journal.pone.0140173 .
237	AMEEN-ALI K E, EACOTT M J, EASTON A. A new behavioural apparatus to reduce animal numbers in multiple types of spontaneous object recognition paradigms in rats[J]. J Neurosci Methods, 2012, 211(1):66-76. DOI: 10.1016/j.jneumeth. 2012.08.006 .
238	PRADILLO J M, DENES A, GREENHALGH A D, et al. Delayed administration of interleukin-1 receptor antagonist reduces ischemic brain damage and inflammation in comorbid rats[J]. J Cereb Blood Flow Metab, 2012, 32(9):1810-1819. DOI: 10.1038/jcbfm.2012.101 .
239	IOANNIDIS J P A. Limitations are not properly acknowledged in the scientific literature[J]. J Clin Epidemiol, 2007, 60(4):324-329. DOI: 10.1016/j.jclinepi.2006.09.011 .
240	PUHAN M A, AKL E A, BRYANT D, et al. Discussing study limitations in reports of biomedical studies- the need for more transparency[J]. Health Qual Life Outcomes, 2012, 10:23. DOI: 10.1186/1477-7525-10-23 .
241	WIESCHOWSKI S, CHIN W W L, FEDERICO C, et al. Preclinical efficacy studies in investigator brochures: do they enable risk-benefit assessment?[J]. PLoS Biol, 2018, 16(4): e2004879. DOI: 10.1371/journal.pbio.2004879 .
242	DIAZ A, PEREZ A, SREEVATSAN S, et al. Association between influenza A virus infection and pigs subpopulations in endemically infected breeding herds[J]. PLoS One, 2015, 10(6): e0129213. DOI: 10.1371/journal.pone.0129213 .
243	DHONDUP Y, SJAASTAD I, SCOTT H, et al. Sustained toll-like receptor 9 activation promotes systemic and cardiac inflammation, and aggravates diastolic heart failure in SERCA2a KO mice[J]. PLoS One, 2015, 10(10): e0139715. DOI: 10.1371/journal.pone.0139715 .
244	CHALMERS I, BRACKEN M B, DJULBEGOVIC B, et al. How to increase value and reduce waste when research priorities are set[J]. Lancet, 2014, 383(9912):156-165. DOI: 10.1016/S0140-6736(13)62229-1 .
245	VOELKL B, VOGT L, SENA E S, et al. Reproducibility of preclinical animal research improves with heterogeneity of study samples[J]. PLoS Biol, 2018, 16(2): e2003693. DOI: 10.1371/journal.pbio.2003693 .
246	MUNAFÒ M R, DAVEY SMITH G. Robust research needs many lines of evidence[J]. Nature, 2018, 553(7689):399-401. DOI: 10.1038/d41586-018-01023-3 .
247	VAN DEN BERGE N, VANHOVE C, DESCAMPS B, et al. Functional MRI during hippocampal deep brain stimulation in the healthy rat brain[J]. PLoS One, 2015, 10(7): e0133245. DOI: 10.1371/journal.pone.0133245 .
248	SACILOTTO N, CASTILLO J, RIFFO-CAMPOS Á L, et al. Growth arrest specific 1 (Gas1) gene overexpression in liver reduces the in vivo progression of murine hepatocellular carcinoma and partially restores gene expression levels[J]. PLoS One, 2015, 10(7): e0132477. DOI: 10.1371/journal.pone. 0132477 .
249	CHAMBERS C D, FEREDOES E, MUTHUKUMARASWAMY S D, et al. Instead of "playing the game" it is time to change the rules: registered Reports at AIMS Neuroscience and beyond[J]. AIMS Neurosci, 2014, 1(1):4-17. DOI: 10.3934/neuro science.2014.1.4 .
250	NOSEK B, LAKENS D. Registered reports: a method to increase the credibility of published results[J]. Soc Psychol, 2014, 45(3):137-141.
251	KAPLAN R M, IRVIN V L. Likelihood of null effects of large NHLBI clinical trials has increased over time[J]. PLoS One, 2015, 10(8): e0132382. DOI: 10.1371/journal.pone.0132382 .
252	ALLEN C, MEHLER D M A. Open science challenges, benefits and tips in early career and beyond[J]. PLoS Biol, 2019, 17(5): e3000246. DOI: 10.1371/journal.pbio.3000246 .
253	NOSEK B A, EBERSOLE C R, DEHAVEN A C, et al. The preregistration revolution[J]. Proc Natl Acad Sci USA, 2018, 115(11):2600-2606. DOI: 10.1073/pnas.1708274114 .
254	MACLEOD M, HOWELLS D. Protocols for laboratory research[J]. Evid Based Preclin Med, 2016, 3(2):e00021. DOI:10.1002/ebm2.21 .
255	PERCIE DU SERT N, BAMSEY I, BATE S T, et al. The experimental design assistant[J]. PLoS Biol, 2017, 15(9): e2003779. DOI: 10.1371/journal.pbio.2003779 .
256	MANTIS C, KANDELA I, AIRD F, et al. Replication Study: Coadministration of a tumor-penetrating peptide enhances the efficacy of cancer drugs[J]. eLife, 2017, 6: e17584. DOI: 10.7554/eLife.17584 .
257	JERÓNIMO S, KHADRAOUI M, WANG D P, et al. Plumage color manipulation has no effect on social dominance or fitness in zebra finches[J]. Behav Ecol, 2018, 29(2):459-467. DOI: 10.1093/beheco/arx195 .
258	SHELDON E L, GRIFFITH S C. Embryonic heart rate predicts prenatal development rate, but is not related to post-natal growth rate or activity level in the zebra finch (Taeniopygia guttata)[J]. Ethology, 2018, 124(11):829-837. DOI: 10.1111/eth.12817 .
259	KAFKAFI N, MAYO C L, ELMER G I. Mining mouse behavior for patterns predicting psychiatric drug classification[J]. Psychopharmacology, 2014, 231(1):231-242. DOI: 10.1007/s00213-013-3230-6 .
260	STODDEN V, GUO P X, MA Z K. Toward reproducible computational research: an empirical analysis of data and code policy adoption by journals[J]. PLoS One, 2013, 8(6): e67111. DOI: 10.1371/journal.pone.0067111 .
261	PIWOWAR H A, DAY R S, FRIDSMA D B. Sharing detailed research data is associated with increased citation rate[J]. PLoS One, 2007, 2(3): e308. DOI: 10.1371/journal.pone. 0000308 .
262	MCKIERNAN E C, BOURNE P E, BROWN C T, et al. How open science helps researchers succeed[J]. eLife, 2016, 5: e16800. DOI: 10.7554/eLife.16800 .
263	Data Citation Synthesis Group. Joint declaration of data citation principles[EB/OL]. MARTONEM, ed. San Diego CA: FORCE11, 2014[2023-10-22]. .
264	WILKINSON M D, DUMONTIER M, JSBRAND JAN AALBERSBERG I, et al. The FAIR Guiding Principles for scientific data management and stewardship[J]. Sci Data, 2016, 3:160018. DOI: 10.1038/sdata.2016.18 .
265	FEDERER L M, LU Y L, JOUBERT D J, et al. Biomedical data sharing and reuse: attitudes and practices of clinical and scientific research staff[J]. PLoS One, 2015, 10(6): e0129506. DOI: 10.1371/journal.pone.0129506 .
266	MAMROT J, LEGAIE R, ELLERY S J, et al. De novo transcriptome assembly for the spiny mouse (Acomys cahirinus)[J]. Sci Rep, 2017, 7(1):8996. DOI: 10.1038/s41598-017-09334-7 .
267	BERO L, ANGLEMYER A, VESTERINEN H, et al. The relationship between study sponsorship, risks of bias, and research outcomes in atrazine exposure studies conducted in non-human animals: systematic review and meta-analysis[J]. Environ Int, 2016, 92-93:597-604. DOI: 10.1016/j.envint. 2015.10.011 .
268	DALBØGE L S, PEDERSEN P J, HANSEN G, et al. A Hamster model of diet-induced obesity for preclinical evaluation of anti-obesity, anti-diabetic and lipid modulating agents[J]. PLoS One, 2015, 10(8): e0135634. DOI: 10.1371/journal.pone. 0135634 .
269	GARCIA DE LA SERRANA D, VIEIRA V L A, ANDREE K B, et al. Development temperature has persistent effects on muscle growth responses in gilthead sea bream[J]. PLoS One, 2012, 7(12): e51884. DOI: 10.1371/journal.pone.0051884 .
270	LUNDH A, SISMONDO S, LEXCHIN J, et al. Industry sponsorship and research outcome[J]. Cochrane Database Syst Rev, 2012, 12: MR000033. DOI: 10.1002/14651858.MR000033.pub2 .
271	POPELUT A, VALET F, FROMENTIN O, et al. Relationship between sponsorship and failure rate of dental implants: a systematic approach[J]. PLoS One, 2010, 5(4): e10274. DOI: 10.1371/journal.pone.0010274 .
272	LEXCHIN J, BERO L A, DJULBEGOVIC B, et al. Pharmaceutical industry sponsorship and research outcome and quality: systematic review[J]. BMJ, 2003, 326(7400):1167-1170. DOI: 10.1136/bmj.326.7400.1167 .
273	KRAUTH D, ANGLEMYER A, PHILIPPS R, et al. Nonindustry-sponsored preclinical studies on statins yield greater efficacy estimates than industry-sponsored studies: a meta-analysis[J]. PLoS Biol, 2014, 12(1): e1001770. DOI: 10.1371/journal.pbio.1001770 .
274	GENCHI M, PRATI P, VICARI N, et al. Francisella tularensis: No evidence for transovarial transmission in the tularemia tick vectors Dermacentor reticulatus and Ixodes ricinus[J]. PLoS One, 2015, 10(8): e0133593. DOI: 10.1371/journal.pone. 0133593 .
275	KOLAHIAN S, SADRI H, SHAHBAZFAR A A, et al. The effects of leucine, zinc, and chromium supplements on inflammatory events of the respiratory system in type 2 diabetic rats[J]. PLoS One, 2015, 10(7): e0133374. DOI: 10.1371/journal.pone. 0133374 .
276	EYRE-WALKER A, STOLETZKI N. The assessment of science: the relative merits of post-publication review, the impact factor, and the number of citations[J]. PLoS Biol, 2013, 11(10): e1001675. DOI: 10.1371/journal.pbio.1001675 .

[1]	Jinhuan MIAO, Xia XU, Lu ZHOU, Haiyan CHENG, Yan HE. Visual Analysis of Animal Experiments on Traditional Chinese Medicine (TCM) Nursing Technology Based on VOSviewer [J]. Laboratory Animal and Comparative Medicine, 2023, 43(6): 626-635.
[2]	Xiaying LI, Yonglu TIAN, Xiaoyu LIU, Xuancheng LU, Guoyuan CHEN, Xiao LU, Yu BAI, Jing GAO, Yao LI, Yusheng WEI, Wanyong PANG, Yufeng TAO. Explanation and Elaboration for the ARRIVE Guidelines 2.0—Reporting Animal Research and In Vivo Experiments (Ⅳ) [J]. Laboratory Animal and Comparative Medicine, 2023, 43(6): 659-668.
[3]	Shuo WANG, Yunhui LÜ, Xiaokang WANG, Zhenhao ZHANG, Yongchun CUI. Construction and Verification of Quality Evaluation Indicator System for Extracorporeal Membrane Oxygenation Animal Experimental Platform [J]. Laboratory Animal and Comparative Medicine, 2023, 43(6): 604-611.
[4]	Xiaoyu LIU, Xuancheng LU, Xiaomeng SHI, Yuzhou ZHANG, Chao LÜ, Guoyuan CHEN, Xiao LU, Yu BAI, Jing GAO, Yao LI, Yonggang LIU, Yufeng TAO, Wanyong PANG. Explanation and Elaboration for the ARRIVE Guidelines 2.0—Reporting Animal Research and In Vivo Experiments (Ⅲ) [J]. Laboratory Animal and Comparative Medicine, 2023, 43(4): 446-456.
[5]	Guoyuan CHEN, Xiao LU, Yu BAI, Lingzhi YU, Ying QIAO, Jian WANG, Jin LU, Xiaoyu LIU, Xuancheng LU, Jing GAO, Yao LI, Wanyong PANG. Explanation and Elaboration of the ARRIVE Guidelines 2.0—Reporting Animal Research and In Vivo Experiments (Ⅱ) [J]. Laboratory Animal and Comparative Medicine, 2023, 43(3): 323-331.
[6]	Jian WANG, Jin LU, Zhengwen MA, Guoyuan CHEN, Xiao LU, Yu BAI, Xiaoyu LIU, Xuancheng LU, Jing GAO, Yao LI, Wanyong Pang. Explanation and Elaboration for the ARRIVE Guidelines 2.0—Reporting Animal Research and In Vivo Experiments (Ⅰ) [J]. Laboratory Animal and Comparative Medicine, 2023, 43(2): 213-224.
[7]	Xiangrong DING, Shurui HUO, Jiejie DAI. Research Progress on Influenza A Virus and Nervous System Disease of Human and Experimental Animals [J]. Laboratory Animal and Comparative Medicine, 2023, 43(2): 180-185.
[8]	Junyan ZHANG, Xiaoyu LIU, Yao LI, Guoyuan CHEN, Xiao LU, Yu BAI, Xuancheng LU, Wanyong PANG, Baojin WU. Introduction to the International Guide for Animal Research Reporting ARRIVE 2.0, and Its Implementation Plan in the Journal [J]. Laboratory Animal and Comparative Medicine, 2023, 43(1): 86-94.
[9]	Xiangmei LIU, Zhongchun MA, Hongkun FU, Feng GAO, Yufeng TAO. Discussion on Expression of Laboratory Accreditation Scope in the Field of Toxicology Testing [J]. Laboratory Animal and Comparative Medicine, 2022, 42(6): 526-530.
[10]	Xiaokang WANG, Ruojin ZHAO, Yunhui LÜ, Guangxin YUE, Shangyu LIU, Ting HE, Peng PENG, Liang MENG, Jubo LI, Baojie ZHANG, Chen SHEN, Yongchun CUI, Xin WANG. A Novel Model for in-vivo Training of Postgraduates on Cardiac Electrical Conduction System Mapping Skills [J]. Laboratory Animal and Comparative Medicine, 2022, 42(5): 466-471.
[11]	Yufei LENG, Wenjing LU, Fengwei WANG. Thinking of Teaching Reform in the Animal Experiment of Operative Surgery in Medical School [J]. Laboratory Animal and Comparative Medicine, 2022, 42(5): 472-476.
[12]	YU Yixiang, CAO Shanshan, FENG Yi, LI Jiarui, WANG Rong. Improvement and Effect Evaluation on Method of Tracheal Instillation in Rats [J]. Laboratory Animal and Comparative Medicine, 2021, 41(6): 543-546.
[13]	CHEN Xiaojuan, LI Wei, WANG Lie. Exploration on Informatization of Laboratory Animal Management Process in Colleges and Universities: The Case of Zhejiang University [J]. Laboratory Animal and Comparative Medicine, 2021, 41(6): 554-558.
[14]	ZHOU Bin, ZHAO Yong, WANG Yabing, LIU Jihong. Analysis on Air Distribution by Computational Fluid Dynamics Simulation in Two Types of Laboratory Animal Barrier Facilities [J]. Laboratory Animal and Comparative Medicine, 2021, 41(3): 252-258.
[15]	CHEN Guoyuan, KANG Kang, LI Xiaoxian, JI Wentao, ZHANG Jinmei, FENG Yanhua, TIAN Qianying, WU Baojin. Application of Experimental Material Inspection in Quality Control of SPF Mice [J]. Laboratory Animal and Comparative Medicine, 2021, 41(1): 61-65.