Explanation and Elaboration for the ARRIVE Guidelines 2.0—Reporting Animal Research and In Vivo Experiments (Ⅳ)

doi:10.12300/j.issn.1674-5817.2023.142

Abstract

Abstract:

Improving the reproducibility of biomedical research results is a major challenge.Transparent and accurate reporting of the research process enables readers to evaluate the reliability of the research results and further explore the experiment by repeating it or building upon its findings. The ARRIVE 2.0 guidelines, released in 2019 by the UK National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs), provide a checklist applicable to any in vivo animal research report. These guidelines aim to improve the standardization of experimental design, implementation, and reporting, as well as the reliability, repeatability, and clinical translatability of animal experimental results. The use of ARRIVE 2.0 guidelines not only enriches the details of animal experimental research reports, ensuring that information on animal experimental results is fully evaluated and utilized, but also enables readers to understand the content expressed by the author accurately and clearly, promoting the transparency and integrity of the fundamental research review process. At present, the ARRIVE 2.0 guidelines have been widely adopted by international biomedical journals. This article is a Chinese translation based on the best practices of international journals following the ARRIVE 2.0 guidelines in international journals, specifically for the complete interpretation of the ARRIVE 2.0 guidelines published in the PLoS Biology journal in 2020 (original text can be found at https://arriveguidelines.org). The fourth part of the article includes the items 1-5 of ARRIVE 2.0 Recommended 11 section, which covers "Abstract" "Background" "Objectives" "Ethical statement" and "Housing and husbandry". Its aim is to promote the full understanding and use of the ARRIVE 2.0 guidelines by domestic researchers, enhance the standardization of experimental animal research and reporting, and promote the high-quality development of experimental animal technology and comparative medicine research in China.

Key words: Animal experiment, ARRIVE 2.0 guidelines, ARRIVE recommended 11, Abstract, Background, Objectives, Ethical statement, Housing and husbandry

CLC Number:

R-332

Xiaying LI, Yonglu TIAN, Xiaoyu LIU, Xuancheng LU, Guoyuan CHEN, Xiao LU, Yu BAI, Jing GAO, Yao LI, Yusheng WEI, Wanyong PANG, Yufeng TAO. Explanation and Elaboration for the ARRIVE Guidelines 2.0—Reporting Animal Research and In Vivo Experiments (Ⅳ)[J]. Laboratory Animal and Comparative Medicine, 2023, 43(6): 659-668.

Figures/Tables 1

References 218

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编号No.	报告信息 Information to report	影响实验动物的示例 Examples of effects on laboratory animals
1	笼/箱/饲养系统（类型和尺寸）	影响行为^[182]和恐惧学习^[183]；水箱颜色影响水生物种的应激^[184-185]
2	食物和水（类型、成分、供应商和获取途径）	影响体重、肿瘤发展、肾病严重程度^[186]和出现帕金森病症状的阈值^[187]；母本的饮食会影响后代的体重^[188]
3	垫料和筑巢材料	影响对应激^[189]和疼痛^[190]的行为反应
4	温度和湿度	改变肿瘤发展进展^[191]；调节斑马鱼的性别分化^[192]
5	卫生（笼具更换/水箱换水频率、材料转移、水质）	影响血压、心率和行为^[193]；增加一个额外的变异源^[194-195]
6	社会环境（群体规模和组成/饲养密度）	损害动物福利^[196]；诱发攻击性行为^[197-198]和应激^[185]
7	生物安全（级别）	动物的微生物状态会导致系统性疾病参数的变化^[199]
8	照明（类型、时间表和强度）	改变免疫和应激反应^[200]
9	环境丰富	减少焦虑^[201-202]、应激^[201-202]和异常重复行为^[203-205]；降低癫痫^[206]和骨关节炎^[207]的易感性，并改变神经系统疾病的病理学^[208]；增加鱼类的觅食行为^[209]
10	实验动物的性别	影响生理应激和疼痛行为^[210]

编号No.	报告信息 Information to report	影响实验动物的示例 Examples of effects on laboratory animals
1	笼/箱/饲养系统（类型和尺寸）	影响行为^[182]和恐惧学习^[183]；水箱颜色影响水生物种的应激^[184-185]
2	食物和水（类型、成分、供应商和获取途径）	影响体重、肿瘤发展、肾病严重程度^[186]和出现帕金森病症状的阈值^[187]；母本的饮食会影响后代的体重^[188]
3	垫料和筑巢材料	影响对应激^[189]和疼痛^[190]的行为反应
4	温度和湿度	改变肿瘤发展进展^[191]；调节斑马鱼的性别分化^[192]
5	卫生（笼具更换/水箱换水频率、材料转移、水质）	影响血压、心率和行为^[193]；增加一个额外的变异源^[194-195]
6	社会环境（群体规模和组成/饲养密度）	损害动物福利^[196]；诱发攻击性行为^[197-198]和应激^[185]
7	生物安全（级别）	动物的微生物状态会导致系统性疾病参数的变化^[199]
8	照明（类型、时间表和强度）	改变免疫和应激反应^[200]
9	环境丰富	减少焦虑^[201-202]、应激^[201-202]和异常重复行为^[203-205]；降低癫痫^[206]和骨关节炎^[207]的易感性，并改变神经系统疾病的病理学^[208]；增加鱼类的觅食行为^[209]
10	实验动物的性别	影响生理应激和疼痛行为^[210]

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