Diagnosis of Trichoepithelioma in a Tree Shrew and Observation of Cell Biological Characteristics

doi:10.12300/j.issn.1674-5817.2022.173

Abstract

Abstract:

Objective To observe the primary tumor of tree shrews and to provide a basis for studying the pathogenesis and prevention of trichoepithelioma. Methods A tumor was discovered in the chest and abdomen of a tree shrew during natural cultivation. The tree shrew was anesthetized, and the tumor was surgically removed. Hematoxylin and eosin (HE) staining and immunohistochemical staining were performed on the tumor tissue after paraffin section, and the tumor cells were isolated and cultured by passage. The isolated tumor cells were subcutaneously injected into healthy tree shrews and nude mice. The tumorigenesis of tumor cells in vivo was observed once a day, with nude mice continuously observed for 2 months and tree shrews observed for more than 6 months. Results HE staining showed that the basal cells in the dermis were arranged as a whole, like a string of petals, forming nests and stripe-like structures with clear boundaries. The observation results after magnification revealed that the tumor cells were arranged in a pallisade-like and basal pattern, with deep nuclear staining and minimal cytoplasmic. Immunohistochemical staining showed the high expression of CK protein and low proportion expression of ki-67 protein in tumor cells, as well as the high expression of vimentin and low expressions of Bcl2 and CD10 in tumor cell mesenchyme. The isolated tumor cells grew well in DMEM medium containing 10% fetal bovine serum and could be cultured by passage, but no tumor formation was observed in healthy tree shrews and nude mice inoculated with tumor cells. Conclusion Combined with the location of the tumor, overall morphology, HE staining, and immunohistochemical results, the thoracoabdominal mass of the tree shrew was diagnosed as a trichoepithelioma.

Key words: Tree shrew, Trichoepithelioma, Diagnosis, Tumor cell transplantation, Nude mice

CLC Number:

R-332

Shanshan ZHAI, Liang LIANG, Yingying CAO, Zhuxin LI, Qing WANG, Junyu TAO, Chenxia YUN, Jing LENG, Haibo TANG. Diagnosis of Trichoepithelioma in a Tree Shrew and Observation of Cell Biological Characteristics[J]. Laboratory Animal and Comparative Medicine, 2023, 43(4): 440-445.

Figures/Tables 3

Figure 1 Histopathological changes of primary tumor in the tree shrews （HE staining）Note： A， The edge of the tumor tissue was clearly outlined and the tumor cells were arranged in a striated and palisade pattern （×50）； B， HE-stained tumor cells were basal cell-like with deep nuclear staining and minimal cytoplasm （×200）.

Figure 2 Immunohistochemistry of trichoepithelioma from a tree shrew（DAB staining，×100）Note： A， Strongly positive expression of CK in tumor cells （As indicated by the red arrow）； B， Strongly positive expression of vimentin in tumor cell mesenchyme （as indicated by the red arrow）； C， Low index expression of ki-67 in tumor cells （as indicated by the red arrow）； D， Low positive expression of Bcl2 in tumor cell mesenchyme （as indicated by the red arrow）； E， Low proportion expression of CD10 in tumor cell mesenchyme （as indicated by the red arrow）； F， Negative expression of CD34 in tumor cells.

Figure 3 Culture of primary tumor cells from a tree shrew trichoepithelioma and morphological observationNote：A， Day 1 after tumor cell isolation and culture （×100）； B， Day 2 after tumor cell isolation and culture （×100）； C， Day 5 after tumor cell isolation and culture （×100）. D， Tumor cells were pleomorphic， irregular， and tentacled （×400）.

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