一种痰湿证慢性阻塞性肺疾病大鼠模型的建立与评价

doi:10.12300/j.issn.1674-5817.2026.001

实验动物与比较医学 ›› 2026, Vol. 46 ›› Issue (5): 668-676.DOI: 10.12300/j.issn.1674-5817.2026.001

一种痰湿证慢性阻塞性肺疾病大鼠模型的建立与评价

张攀攀¹^,², 许金森², 程佑民³, 吴华嵩³, 林静瑜¹^,²()()

^1. 福建省中医药科学院药物研究所, 福州 350003
^2. 福建省中医药科学院经络感传重点实验室, 福州 350003
^3. 福建省中医药科学院实验动物中心, 福州 350003

收稿日期:2026-01-03 修回日期:2026-06-11 出版日期:2026-10-25 发布日期:2026-10-16
通讯作者: 林静瑜（1973—），女，博士，副研究员，研究方向：针药结合的效应机制研究。E-mail： jingyul2012@163.com。ORCID： 0009-0001-4292-1129
作者简介:
张攀攀（1994—），女，硕士研究生，研究方向：针灸的效应机制研究。E-mail： 1397002257@qq.com
基金资助:
国家自然科学基金“基于人工智能辅助分析探讨循经感传与针刺靶向趋病的关系”(82274413); 福建省科技计划项目“卤地菊对脓毒症急性肺损伤的保护作用及其NLRP3炎症小体机制”(2022R1003006)

Establishment and Evaluation of a Rat Model of Chronic Obstructive Pulmonary Disease with Phlegm-Dampness Syndrome

ZHANG Panpan¹^,², XU Jinsen², CHENG Youmin³, WU Huasong³, LIN Jingyu¹^,²()()

^1. Institute of Materia Medica, Fujian Academy of Chinese Medical Sciences, Fuzhou 350003, China
^2. Key Laboratory of Propagated Sensation Along Meridian, Fujian Academy of Chinese Medical Sciences, Fuzhou 350003, China
^3. Institute of Laboratory Animal Science, Fujian Academy of Chinese Medical Sciences, Fuzhou 350003, China

Received:2026-01-03 Revised:2026-06-11 Published:2026-10-25 Online:2026-10-16
Correspondence to:
LIN Jingyu (ORCID:0009-0001-4292-1129), E-mail: jingyul2012@163.com

摘要/Abstract

摘要：

目的建立一种符合中医痰湿证的慢性阻塞性肺疾病（chronic obstructive pulmonary disease，COPD）病证结合模型，并进行表型评价。方法将18只SPF级8周龄雄性SD大鼠，随机分为对照组（n=8）和模型组（n=10）。造模耗时19周，对照组无干预。造模方法：分别于第1、5、9、13、17周（滴注周）的起始日上午9：00，向大鼠行气管内滴注脂多糖（lipopolysaccharide，LPS），共5次，每次给药剂量为40 μg/只，当日给药1次。除滴注周外，每日于自制高湿环境的烟熏箱内，让大鼠接受香烟烟雾暴露（第9周开始前15支香烟/次，自第9周起20支香烟/次，每日30 min/次）。每周测量一次大鼠体重，观察其一般体征及痰湿证表征。造模结束次日麻醉大鼠，以进行肺通气功能测定。采用酶联免疫吸附试验（enzyme linked immunosorbent assay，ELISA）检测血清、支气管肺泡灌洗液（bronchoalveolar lavage fluid，BALF）中肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）和黏蛋白5AC（mucin 5AC，MUC5AC）的含量；对肺组织进行HE染色和阿尔辛蓝-过碘酸希夫染色（alcian blue and periodic acid-Schiff staining，AB-PAS），以观察肺组织形态结构、杯状细胞的增生情况；采用蛋白质印迹法检测肺组织中TNF-α、MUC5AC蛋白的表达量。结果体征与一般状态方面，与对照组比较，模型组大鼠体重自第3周起均显著减轻（P<0.01），并出现毛色发黄、活动迟缓、懒动、呼吸喘促及口鼻分泌物增多等现象。肺通气功能方面，与对照组比较，模型组大鼠肺潮气量、每分通气量均显著下降（P<0.01），动态顺应性显著降低（P<0.05），而肺弹性阻力显著增加（P<0.01）。炎症因子和黏液相关蛋白方面，与对照组比较，模型组大鼠的血清、BALF和肺组织中TNF-α、MUC5AC蛋白水平均显著增高（P<0.01）。病理形态学方面，HE染色结果显示，模型组大鼠肺泡明显扩张、破裂并部分融合成肺大疱，细支气管腔内有炎性细胞浸润；AB-PAS染色结果显示，模型组大鼠细支气管黏膜上皮杯状细胞增多，腔内形成黏液栓。结论本造模方法可复制出符合中医痰湿证症状且具有肺通气功能下降与肺气肿、炎症、慢性支气管炎样病理特征的COPD大鼠模型，可为后续中医药治疗痰湿证COPD研究提供可靠支撑。

关键词: 慢性阻塞性肺疾病, 痰湿证, 病证结合模型, 肿瘤坏死因子-α, 黏蛋白5AC, SD大鼠

Abstract:

Objective To establish and evaluate a rat model of chronic obstructive pulmonary disease (COPD) associated with the traditional Chinese medicine (TCM) phlegm-dampness syndrome. Methods Eighteen SPF-grade 8-week-old male SD rats were randomly assigned to a control group (n=8) and a model group (n=10). The modeling procedure lasted for 19 weeks. Rats in the control group received no intervention. Lipopolysaccharide (LPS; 40 μg per rat per instillation) was instilled intratracheally into rats in the model group at 09:00 on the ﬁrst day of weeks 1, 5, 9, 13, and 17, for a total of ﬁve instillations. Except for the instillation weeks, rats were exposed daily to cigarette smoke in a self-made high-humidity smoke exposure chamber (15 cigarettes/session before week 9 and 20 cigarettes/session from week 9 onward; 30 min/session/day). Body weight was recorded weekly, and general signs and manifestations of TCM phlegm-dampness syndrome were observed. On the day after modeling, rats were anesthetized for pulmonary ventilatory function testing. Tumor necrosis factor-α (TNF-α) and mucin 5AC (MUC5AC) levels in serum and bronchoalveolar lavage fluid (BALF) were measured using enzyme-linked immunosorbent assay (ELISA). Lung tissues were subjected to hematoxylin and eosin (HE) and alcian blue and periodic acid-Schiff staining (AB-PAS) to assess lung tissue morphology and goblet cell hyperplasia. Western blotting was performed to determine TNF-α and MUC5AC protein expression in lung tissue. Results General signs and overall condition: Compared with the control group, body weight in the model group was significantly decreased from week 3 onward (P<0.01). Rats showed yellowish fur, sluggish activity, reduced movement, rapid/labored breathing, and increased oral/nasal secretions. Pulmonary ventilatory function: Compared with the control group, minute ventilation and tidal volume (both P<0.01) and dynamic compliance (P<0.05) were decreased, whereas elastic resistance was markedly increased (P<0.01) in the model group. Inflammatory factors and mucus-related proteins: Compared with the control group, TNF-α and MUC5AC levels in serum and BALF, as well as their protein levels in lung tissue, were significantly elevated in the model group (all P<0.01). Histopathology: HE staining showed obvious alveolar dilatation and rupture with partial coalescence into bullae, and inflammatory cell infiltration in bronchiolar lumens. AB-PAS staining revealed increased goblet cells in bronchiolar mucosal epithelium and mucus plug formation in the lumen. Conclusion This protocol can reproduce a COPD rat model with manifestations consistent with TCM phlegm-dampness syndrome, reduced pulmonary ventilation function, and pathological features of emphysema, inflammation, and chronic bronchitis, thereby providing reliable support for subsequent studies on TCM treatment of COPD with phlegm-dampness syndrome.

Key words: Chronic obstructive pulmonary disease, Phlegm-dampness syndrome, Disease-syndrome combination model, Tumor necrosis factor-α, Mucin 5AC, SD rat

中图分类号:

R-332
R563.9

张攀攀,许金森,程佑民,等. 一种痰湿证慢性阻塞性肺疾病大鼠模型的建立与评价[J]. 实验动物与比较医学, 2026, 46(5): 668-676. DOI: 10.12300/j.issn.1674-5817.2026.001.

ZHANG Panpan,XU Jinsen,CHENG Youmin,et al. Establishment and Evaluation of a Rat Model of Chronic Obstructive Pulmonary Disease with Phlegm-Dampness Syndrome[J]. Laboratory Animal and Comparative Medicine, 2026, 46(5): 668-676. DOI: 10.12300/j.issn.1674-5817.2026.001.

图/表 6

表1

慢性阻塞性肺疾病痰湿证模型大鼠的中医证候积分量表

Table 1

Traditional Chinese medicine syndrome scoring scale for rats with phlegm-dampness syndrome in chronic obstructive pulmonary disease models

评分

Score

大鼠行为

Rat behavior

精神状态

Mental state

毛发、耳、尾

Hair, ear, tail

舌苔

Tongue coating

饮食、体重

Diet and body weight

图1 两组大鼠体重增长变化比较和烟熏状态图

注：A图为两组大鼠体重增长折线图（ $x ¯$ ±s，n=8）。与对照组比较，^* P<0.05，^** P<0.01。B图为两组大鼠外形比较（实验第19周时大鼠图片）。C图为模型组大鼠烟熏时状态。

Figure 1 Comparison of body weight gain changes between the two groups and smoke exposure status in rats

Note： A， Body weight gain line chart of rats in the two groups （ $x ¯$ ±s，n=8）. Compared with the control group， ^* P<0.05， ^** P<0.01. B， Gross appearance comparison between the two groups of rats （Photographs of rats at week 19 of the experiment）. C， Status of rats in the model group during smoke exposure.

图1 两组大鼠体重增长变化比较和烟熏状态图

Figure 1 Comparison of body weight gain changes between the two groups and smoke exposure status in rats

图2 两组大鼠肺动态顺应性、弹性阻力、每分通气量和潮气量比较

注：A图为大鼠肺顺应性；B图为大鼠肺弹性阻力；C图为大鼠每分通气量；D图为大鼠潮气量。与对照组比较，^* P<0.05；^** P<0.01。n=6。

Figure 2 Comparison of pulmonary dynamic compliance， elastic resistance， minute ventilation， and tidal volume between the two groups of rats

Note： A， Pulmonary dynamic compliance in rats； B， Lung elastic resistance in rats； C， Minute ventilation volume in rats； D， Tidal volume in rats. n=6 rats per group. Compared with the control group， ^* P<0.05， ^** P<0.01. n=6.

图3 两组大鼠血清和支气管肺泡灌洗液中肿瘤坏死因子-α、黏蛋白5AC含量比较

注：A图为大鼠血清中肿瘤坏死因子-α（TNF-α）的浓度比较；B图为大鼠血清中黏蛋白5AC（MUC5AC）的浓度比较；C图为大鼠支气管肺泡灌洗液（BALF）中TNF-α的浓度比较；D图为大鼠支气管肺泡灌洗液中MUC5AC的浓度比较。模型组8只大鼠，与对照组比较，^** P<0.01。

Figure 3 Comparison of TNF-α and MUC5AC levels in serum and BALF between the two groups of rats

Note： A， Comparison of tumor necrosis factor-α （TNF-α） levels in rat serum； B， Comparison of mucin 5AC （MUC5AC） levels in rat serum； C， Comparison of TNF-α levels in rat bronchoalveolar lavage fluid （BALF）； D， Comparison of MUC5AC levels in rat BALF. Model group （n=8）. Compared with the control group， ^** P<0.01.

图4 两组大鼠肺组织形态结构比较

注：A为肺HE染色（比例尺从左到右依次为250 μm、100 μm、100 μm和50 μm）；B为肺组织阿尔辛蓝-过碘酸希夫染色（AB-PAS）（比例尺从左到右依次100 μm、50 μm、100 μm和50 μm）。

Figure 4 Comparison of the morphological structure of lung tissue between two groups of rats

Note： A， Hematoxylin and eosin （HE） staining of lung tissue （scale bars from left to right are 250 μm， 100 μm， 100 μm， and 50 μm）； B， Alcian blue and periodic acid-Schiff staining （AB-PAS） of lung tissue （scale bars from left to right are 100 μm， 50 μm， 100 μm， and 50 μm）.

图5 两组大鼠肺组织中肿瘤坏死因子-α、黏蛋白5AC蛋白表达比较

注：A图为蛋白电泳条带图，B图为肺组织中肿瘤坏死因子-α（TNF-α）蛋白表达水平分析，C图为肺组织中黏蛋白5AC（MUC5AC）蛋白表达水平分析，每组8只大鼠；与对照组比较，^** P<0.01。

Figure 5 Comparison of TNF-α and MUC5AC protein expres-sion in lung tissue between the two groups of rats

Note： A， Representative Western blot bands； B， Relative TNF-α protein expression in lung tissue； C， Relative mucin 5AC （MUC5AC） protein expression in lung tissue. n=8 rats per group. Compared with the control group， ^** P<0.01.

参考文献 28

[1]	BOERS E, ALLEN A, BARRETT M, et al. Forecasting the global economic and health burden of COPD from 2025 through 2050[J]. Chest, 2025, 168(4): 880-889. DOI: 10.1016/j.chest. 2025.03.029.
[2]	AGUSTÍ A, CELLI B R, CRINER G J, et al. Global initiative for chronic obstructive lung disease 2023 report: gold executive summary[J]. Eur Respir J, 2023, 61(4): 2300239. DOI: 10.1183/13993003.00239-2023.
[3]	李建生. 国际中医临床实践指南慢性阻塞性肺疾病[J]. 世界中医药, 2020, 15(7): 1084-1092. DOI: 10.3969/j.issn.1673-7202.2020.07.026.
	LI J S. International clinical practice guideline of Chinese medicine chronic obstructive pulmonary disease[J]. World Chin Med, 2020, 15(7): 1084-1092. DOI: 10.3969/j.issn.1673-7202.2020.07.026.
[4]	王笑铭, 孟晨晨, 范鹿, 等. 中医证候类动物模型构建方法概述[J]. 实验动物与比较医学, 2025, 45(5): 596-610. DOI: 10.12300/j.issn.1674-5817.2025.038.
	WANG X M, MENG C C, FAN L, et al. An overview of strategies for constructing animal models of traditional Chinese medicine syndromes[J]. Lab Anim Comp Med, 2025, 45(5): 596-610. DOI: 10.12300/j.issn.1674-5817.2025.038.
[5]	冯硕, 胡晶, 张会娜, 等. 中药新药研发中人用经验数据的评价方法探索及思考[J]. 中国中药杂志, 2022, 47(6): 1700-1704. DOI: 10.19540/j.cnki.cjcmm.20220105.501.
	FENG S, HU J, ZHANG H N, et al. Exploration and reflection on evaluation methodology of human use experience for new Chinese drug development[J]. China J Chin Mater Med, 2022, 47(6): 1700-1704. DOI: 10.19540/j.cnki.cjcmm.20220105.501.
[6]	黄锦榕, 洪敏俐, 李灿东, 等. 基于聚类分析的慢阻肺稳定期中医证型分布特征及其与临床综合指标相关性研究[J]. 亚太传统医药, 2018, 14(5): 107-111. DOI: 10.11954/ytctyy.201805038.
	HUANG J R, HONG M L, LI C D, et al. Study of distribution characteristics of TCM syndromes in the stable COPD based on the cluster analysis and its correlation with clinical comprehensive indexes[J]. Asia Pac Tradit Med, 2018, 14(5): 107-111. DOI: 10.11954/ytctyy.201805038.
[7]	邵雪洁, 徐可心, 廖一羲, 等. 呼吸系统疾病病证结合动物模型的研究进展[J]. 中华中医药杂志, 2024, 39(6): 2982-2986.
	SHAO X J, XU K X, LIAO Y X, et al. Research progress of animal models of combination of disease and syndrome in respiratory diseases[J]. China J Tradit Chin Med Pharm, 2024, 39(6): 2982-2986.
[8]	黄培炜, 林静瑜, 周凡, 等. 尺泽穴位注射对慢阻肺大鼠气道组织形态学和氧化水平的影响[J]. 临床肺科杂志, 2020, 25(11): 1667-1671. DOI: 10.3969/j.issn.1009-6663.2020.11.010.
	HUANG P W, LIN J Y, ZHOU F, et al. Effect of LU5 point injection on airway histomorphology and oxidative stress in rats with COPD[J]. J Clin Pulm Med, 2020, 25(11): 1667-1671. DOI: 10.3969/j.issn.1009-6663.2020.11.010.
[9]	刘思美, 强喆, 吴思澜, 等. 痰湿阻肺型COPD大鼠模型的构建及代谢组学分析[J]. 中国实验动物学报, 2023, 31(1): 51-63. DOI: 10.3969/j.issn.1005-4847.2023.01.007.
	LIU S M, QIANG Z, WU S L, et al. Establishment and metabolomics analysis of a rat model of chronic obstructive pulmonary disease with phlegm-dampness syndrome[J]. Acta Lab Anim Sci Sin, 2023, 31(1): 51-63. DOI: 10.3969/j.issn.1005-4847.2023.01.007.
[10]	春柳, 李建生, 马锦地, 等. 基于现代名老中医经验的痰饮常见证候及其特征的研究[J]. 辽宁中医杂志, 2018, 45(3): 496-498. DOI: 10.13192/j.issn.1000-1719.2018.03.013.
	CHUN L, LI J S, MA J D, et al. Study on the common syndromes and characteristics of phlegm and drink based on the experience of modern famous and old Chinese medicine practitioners[J]. Liaoning J Tradit Chin Med, 2018, 45(3): 496-498. DOI: 10.13192/j.issn.1000-1719.2018.03.013.
[11]	罗齐军, 陈媛丽, 夏燕华, 等. 三子六君汤联合穴位贴敷治疗慢阻肺急性加重期临床研究[J]. 亚太传统医药, 2017, 13(14): 142-144. DOI: 10.11954/ytctyy.201714066.
	LUO Q J, CHEN Y L, XIA Y H, et al. Clinical observation of AECOPD treated by San zi Liu Jun decoction combined with acupoint application of Chinese herbal medicine[J]. Asia Pac Tradit Med, 2017, 13(14): 142-144. DOI: 10.11954/ytctyy.201714066.
[12]	李会革, 王迪浔. 实验动物呼吸力学指标的测定[J]. 同济医科大学学报, 1995, 24(2): 91-93.
	LI H G, WANG D X. Measurement of respiratory mechanics in experimental animals[J]. Acta Univ Med Tongji, 1995, 24(2): 91-93.
[13]	陈美凤, 加慧, 夏书月. 不同COPD动物模型特点及应用的研究进展[J]. 中国实验动物学报, 2020, 28(4): 563-569. DOI: 10.3969/j.issn.1005-4847.2020.04.019.
	CHEN M F, JIA H, XIA S Y. Research progress on the characteristics of animal models of chronic obstructive pulmonary disease and related clinical applications[J]. Acta Lab Anim Sci Sin, 2020, 28(4): 563-569. DOI: 10.3969/j.issn.1005-4847.2020.04.019.
[14]	刘迪, 张洪春. 慢性阻塞性肺疾病基因工程动物模型研究进展[J]. 中国生物工程杂志, 2020, 40(4): 59-68. DOI: 10.13523/j.cb.1909072.
	LIU D, ZHANG H C. Advances in genetically engineered animal models of chronic obstructive pulmonary disease[J]. China Biotechnol, 2020, 40(4): 59-68. DOI: 10.13523/j.cb.1909072.
[15]	张泽宇, 梅晓峰, 陶柳颖, 等. 香烟烟雾联合肺炎克雷伯杆菌诱导慢阻肺模型小鼠气道重塑机制[J]. 中国实验动物学报, 2025, 33(5): 644-655. DOI: 10.3969/j.issn.1005-4847.2025.05.003.
	ZHANG Z Y, MEI X F, TAO L Y, et al. Mechanism of airway remolding in a mouse model of chronic obstructive pulmonary disease induced by cigarette smoke combined with Klebsiella pneumoniae[J]. Acta Lab Anim Sci Sin, 2025, 33(5): 644-655. DOI: 10.3969/j.issn.1005-4847.2025.05.003.
[16]	李武, 刘雲华, 石拓, 等. LPS+CS诱导12周建立符合临床标准的COPD小鼠模型[J]. 陆军军医大学学报, 2023, 45(9): 982-988. DOI: 10.16016/j.2097-0927.202301084.
	LI W, LIU Y H, SHI T, et al. Establish of a mouse model of COPD that meets clinical criteria by LPS+ cigarette smoke exposure for 12 weeks[J]. J Army Med Univ, 2023, 45(9): 982-988. DOI: 10.16016/j.2097-0927.202301084.
[17]	邓铁涛. 中医诊断学[M]. 3版. 上海: 上海科学技术出版社, 2013.
	DENG T T. Diagnostics of traditional Chinese medicine[M]. 3rd ed. Shanghai: Shanghai Scientific and Technical Publishers, 2013.
[18]	YUK J M, KIM J K, KIM I S, et al. TNF in human tuberculosis: a double-edged sword[J]. Immune Netw, 2024, 24(1): e4. DOI: 10.4110/in.2024.24.e4.
[19]	SONG K S, LEE W J, CHUNG K C, et al. Interleukin-1β and tumor necrosis factor-α induce MUC5AC overexpression through a mechanism involving ERK/p38 mitogen-activated protein kinases-MSK1-CREB activation in human airway epithelial cells[J]. J Biol Chem, 2003, 278(26): 23243-23250. DOI: 10.1074/jbc.M300096200.
[20]	LORA J M, ZHANG D M, LIAO S M, et al. Tumor necrosis factor-α triggers mucus production in airway epithelium through an IκB kinase β-dependent mechanism[J]. J Biol Chem, 2005, 280(43): 36510-36517. DOI: 10.1074/jbc.M507977200.
[21]	FU H T, ZHANG Y, ZHANG P, et al. Tumor necrosis factor-α promotes airway mucus hypersecretion by repressing miR-146a-5p and miR-134-5p levels in human airway epithelial cells[J]. Transl Cancer Res, 2021, 10(9): 4047-4056. DOI: 10.21037/tcr-20-3375.
[22]	YAO Y, ZHOU J, DIAO X, et al. Association between tumor necrosis factor-α and chronic obstructive pulmonary disease: a systematic review and meta-analysis[J]. Ther Adv Respir Dis, 2019, 13: 1753466619866096. DOI: 10.1177/1753466619866096.
[23]	杜利莉, 章丹宁, 薛梅, 等. 中医证候动物模型建立及评价方法研究[J]. 南京中医药大学学报, 2025, 41(1): 11-18. DOI: 10.14148/j.issn.1672-0482.2025.0011.
	DU L L, ZHANG D N, XUE M, et al. Research on establishment and evaluation methods of animal model of TCM syndromes[J]. J Nanjing Univ Tradit Chin Med, 2025, 41(1): 11-18. DOI: 10.14148/j.issn.1672-0482.2025.0011.
[24]	林浩. 慢性阻塞性肺病寒饮蕴肺证大鼠模型血清细胞因子表达的动态研究[D]. 济南: 山东中医药大学, 2014.
	LIN H. Dynamic study on serum cytokine expressions in rat models of chronic obstructive pulmonary disease with cold‑fluid accumulating in lung syndrome[D]. Jinan: Shandong University of Traditional Chinese Medicine, 2014.
[25]	孙广仁, 王洪武, 高博, 等. COPD寒饮蕴肺证大鼠病证结合模型的建立[J]. 山东中医药大学学报, 2007, 31(3): 242-244. DOI: 10.3969/j.issn.1007-659X.2007.03.025.
	SUN G R, WANG H W, GAO B, et al. Establishment of a disease-syndrome combined rat model of COPD with cold fluid retention in lung syndrome[J]. J Shandong Univ Tradit Chin Med, 2007, 31(3): 242-244. DOI: 10.3969/j.issn.1007-659X.2007.03.025.
[26]	高旅, 刘丽娜, 史正刚. 从中医角度看气道黏液高分泌[J]. 中国中西医结合儿科学, 2019, 11(2): 133-135. DOI: 10.3969/j.issn.1674-3865.2019.02.011.
	GAO L, LIU L N, SHI Z G. Airway mucus hypersecretion from the perspective of traditional Chinese medicine[J]. Chin Pediatr Integr Tradit West Med, 2019, 11(2): 133-135. DOI: 10.3969/j.issn.1674-3865.2019.02.011.
[27]	RADICIONI G, CEPPE A, FORD A A, et al. Airway mucin MUC5AC and MUC5B concentrations and the initiation and progression of chronic obstructive pulmonary disease: an analysis of the SPIROMICS cohort[J]. Lancet Respir Med, 2021, 9(11): 1241-1254. DOI: 10.1016/S2213-2600(21)00079-5.
[28]	LI J Y, YE Z G. The potential role and regulatory mechanisms of MUC5AC in chronic obstructive pulmonary disease[J]. Molecules, 2020, 25(19): 4437. DOI: 10.3390/molecules25194437.

一种痰湿证慢性阻塞性肺疾病大鼠模型的建立与评价

Establishment and Evaluation of a Rat Model of Chronic Obstructive Pulmonary Disease with Phlegm-Dampness Syndrome

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 28

相关文章 15

编辑推荐

Metrics

本文评价

[1]	张海涛, 魏雅岚, 范晓静, 陈璐, 庄旭东, 王心睿. 畲药十二时辰的三萜皂苷富集组分对宫颈炎模型大鼠的抗炎作用及其机制探索[J]. 实验动物与比较医学, 2026, 46(5): 616-628.
[2]	田艳, 赖冬, 赖飞, 姬长甫. 脓毒症大鼠模型肝素结合蛋白含量变化与凝血功能紊乱和血管内皮损伤的关系[J]. 实验动物与比较医学, 2026, 46(5): 700-706.
[3]	李杨锦, 吴珍红, 黄宗锈. 灵芝孢子油对大鼠28天经口毒性试验研究[J]. 实验动物与比较医学, 2026, 46(5): 660-667.
[4]	李燕平, 黄姗, 姚政源, 石怡敏, 隋华秀, 黄侯丹, 俞燕萍, 米燕, 林大晖. 艾黄素对臭氧暴露诱发小鼠肺部炎症的保护作用及机制研究[J]. 实验动物与比较医学, 2026, 46(5): 640-649.
[5]	陈婉琳, 董庆睿, 李孟淘, 林楠, 赵虎, 张再重, 曾令军, 李佩, 杨劲, 王瑜, 黄爱文. 纳米碳-二氧化锰注射液在大鼠和猪胃肠道染色标记定位中的应用[J]. 实验动物与比较医学, 2026, 46(5): 727-739.
[6]	练荣贵, 廖乃顺. 稀土下转换纳米颗粒标记脂肪间充质干细胞治疗亚急性肝损伤的活体示踪研究[J]. 实验动物与比较医学, 2026, 46(5): 716-726.
[7]	潘林琴, 邓向亮, 罗云霞. 缺血性脑卒中动物模型的中西医整合转化研究进展[J]. 实验动物与比较医学, 2026, 46(3): 344-356.
[8]	艾秀峰, 张利棕, 方明笋, 吕东颖, 陈楚, 蔡兆伟, 王德军. 16S rRNA测序分析饮用含氯水对大鼠和小鼠肠道菌群的影响差异[J]. 实验动物与比较医学, 2026, 46(3): 437-445.
[9]	何家祺, 周园园, 聂永强, 王朝霞, 徐汪节. 香烟烟雾暴露诱导慢性阻塞性肺疾病小鼠模型的构建及多维度病理评估[J]. 实验动物与比较医学, 2026, 46(1): 11-19.
[10]	秦超, 李双星, 赵婷婷, 蒋晨晨, 赵晶, 杨艳伟, 林志, 王三龙, 文海若. 药物安全评价用SD大鼠90 d喂养试验的背景数据研究[J]. 实验动物与比较医学, 2025, 45(4): 439-448.
[11]	潘颐聪, 蒋汶洪, 胡明, 覃晓. 慢性肾脏病大鼠主动脉钙化模型的术式优化及效果评价[J]. 实验动物与比较医学, 2025, 45(3): 279-289.
[12]	葛健, 孙静芬, 吴永杰. 牛磺酸对苯扎氯铵损伤大鼠眼角膜内皮细胞无保护作用[J]. 实验动物与比较医学, 2023, 43(1): 39-43.
[13]	许琴, 倪艳, 是文辉, 李建瑛, 刘江伟, 赵红琼, 徐新明. SPF级雄性SD大鼠回肠与结肠菌群结构比较分析[J]. 实验动物与比较医学, 2023, 43(1): 53-60.
[14]	张笑瑞, 曹静, 吴倩倩, 刘季俊, 陈国元, 吴宝金. 不同剂型普罗布考对大鼠肠系膜淋巴转运及药代动力学的影响[J]. 实验动物与比较医学, 2022, 42(4): 275-283.
[15]	赵斯佳, 何新宇, 景泉, 马林, 郭春岚, 万阔. 大鼠急性牙髓炎痛敏模型的疼痛效应评价[J]. 实验动物与比较医学, 2022, 42(4): 333-341.