不同剂量电离辐射对小鼠睾丸的损伤效应

doi:10.3969/j.issn.1674-5817.2013.04.003

实验动物与比较医学 ›› 2013, Vol. 33 ›› Issue (4): 261-264.DOI: 10.3969/j.issn.1674-5817.2013.04.003

不同剂量电离辐射对小鼠睾丸的损伤效应

刘李娜¹, 周茜², 孙欢², 唐瑛¹

1.海军医学研究所, 上海 200433;
2.湖北中医药大学, 武汉 430065

收稿日期:2013-05-07 出版日期:2013-08-25 发布日期:2013-08-25
作者简介:刘李娜(1980-),女,硕士,E-mail:liulina0106@sina.com
基金资助:
全军中医药专项(10A023)

Injury Effects of Different Dosage of Ionizing Radiation on Testicles of Male Mice

LIU Li-na¹, ZHOU Xi², CHEN Ya-lin¹, SUN Huan², TANG Ying¹

1. Naval Medical Research Institute, Shanghai 200433, China;
2. Hubei University of Traditional Chinese Medicine, Wuhan 430065, China

Received:2013-05-07 Published:2013-08-25 Online:2013-08-25

摘要/Abstract

摘要： 目的探讨不同剂量电离辐射对小鼠睾丸的损伤效应。方法 120只雄性昆明小鼠,随机分为对照组,2.5 Gy、3.5 Gy、4.5 Gy和5.5 Gy ⁶⁰Coγ射线照射组,每组24只。⁶⁰Coγ照射后3 d、7 d和14 d测定睾丸指数、附睾指数、精子数和精子畸形率,苏木精-伊红染色法观察小鼠睾丸组织病理学变化。结果辐照后3 d, 照射组小鼠附睾指数上升,随后下降,其中辐照后14 d, 4.5 Gy和5.5 Gy组与对照组或同组3 d时比较均差异明显(P<0.01或P <0.05); 各照射组睾丸指数在辐照后7 d和14 d均明显下降(P<0.01或P<0.05), 与辐照后3 d比较5.5 Gy组下降明显(P<0.01)。各照射组的精子畸形率在3 d、7 d和14 d时均明显上升(P<0.01或P<0.05),其中5.5 Gy组上升较快,精子数也下降明显(P<0.01),5.5 Gy组生精小管肌样细胞及基膜均模糊不清。2.5 Gy组辐照后与对照组比较无明显差异。结论辐照对小鼠睾丸造成的损伤程度与辐照剂量呈正相关,其中3.5 Gy和4.5 Gy是较适合制作小鼠睾丸辐射损伤模型的剂量。

关键词: 小鼠, 睾丸, 电离辐射, 辐射损伤

Abstract: Objective To study the injury effects of different dosage of ionizing radiation on the testicles of mice. Methods One hundred and twenty male KM mice were randomily divided into five groups at the radiation dosage of the control(0 Gy), 2.5 Gy, 3.5 Gy, 4.5 Gy and 5.5 Gy respectively. On day 3, day 7 and day 14 after ⁶⁰Coγ exposure, the epididymis index, testicle index, comparative sperm count and sperm abnormality rate were determined. The pathological changes of the testes were observed by hematoxylin and eosin stain. Results On day 3 after exposure, the epididymis index was increased, but it was decreased subsequently. The epididymis index of 4.5 Gy and 5.5 Gy groups was presented significant differences on day 14 after exposure, compared with that of the control group and day 3 group (P<0.01or P<0.05). The testicle index in each group was decreased obviously on day 7 and 14 after exposure (P<0.01or P<0.05). As to 5.5 Gy group, the testicle index was decreased significantly compared with that on day 3 after exposure (P<0.01). The sperm abnormality rate of all groups, especially in 5.5 Gy group increased significantly on day 3, 7 and 14 after exposure (P<0.01or P<0.05). The comparative sperm count of 5.5 Gy group was decreased evidently (P<0.01) and the myoid cells and basal membrane of seminiferous tubules were appeared blurry. All the index of 2.5 Gy group had no significant difference compared with those of the control group. Conclusion The injury degree of testicles in mice is positively correlated with the radiation dosage. The 3.5 Gy and 4.5 Gy are the suitable doses for establishing the model of pathological injuries in the testicles of mice.

Key words: Mice, Testicle, Ionizing radiation, Radiation injury

中图分类号:

Q95-33

LIU Li-na,ZHOU Xi,CHEN Ya-lin,等. 不同剂量电离辐射对小鼠睾丸的损伤效应[J]. 实验动物与比较医学, 2013, 33(4): 261-264. DOI: 10.3969/j.issn.1674-5817.2013.04.003.

刘李娜,周茜,孙欢,et al. Injury Effects of Different Dosage of Ionizing Radiation on Testicles of Male Mice[J]. Laboratory Animal and Comparative Medicine, 2013, 33(4): 261-264. DOI: 10.3969/j.issn.1674-5817.2013.04.003.

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不同剂量电离辐射对小鼠睾丸的损伤效应

Injury Effects of Different Dosage of Ionizing Radiation on Testicles of Male Mice

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