慢性疲劳综合征大鼠模型相关指标观测

doi:10.3969/j.issn.1674-5817.2018.04.007

摘要/Abstract

摘要： 目的采用拥挤等复合应激制作慢性疲劳综合征(CFS)大鼠模型,并观测相关指标。方法综合运用不定期的拥挤、噪音和昼夜颠倒等不同的应激因子长期温和刺激大鼠,造模持续64 d。观察大鼠精神状态、皮毛色泽,每周测体质量,每月进行半定量评分和摄食量测定,采血检测血常规、免疫球蛋白G(IgG)及血乳酸的含量,试验期末强迫游泳测定不动时间。结果与正常对照组比较,造模大鼠自主活动减少、精神萎靡、皮肤松弛、毛发变黄、耳尾颜色淡红少泽,半定量评分值升高;体质量、摄食量下降;白细胞、红细胞、血红蛋白、红细胞比容等下降;强迫游泳后不动时间、血乳酸和IgG上升。结论采用拥挤、噪音和睡眠紊乱成功构建CFS大鼠模型,半定量评分、大鼠体质量、摄食量、白细胞、红细胞、血红蛋白、红细胞比容、强迫游泳后不动时间、血乳酸和IgG可以作为参考指标。

关键词: 慢性疲劳综合征(CFS), 指标, 大鼠

Abstract: Objective To make a rat model of chronic fatigue syndrome (CFS) by overcrowding and other stresses,and to test the related indicators.Methods The rats were stimulated by different stress factors,such as irregular crowding,noise and diurnal reversal for 64 days.The mental status,coat and skin color were observed.Weight and food intake were measured every week.Blood routine,immunoglobulin G (IgG) and blood lactate were measured regularly.Results After modeling,rat independent activity decreased.Mental fatigue,skin loosed,body hair withered,and semi-quantitative score value increased.Body weight and food intake decreased.White blood cell,red blood cell,hemoglobin and erythrocyte specific volume decreased.Inactive time after forced swimming,blood lactic acid and IgG increased.Conclusion The rat models of CFS were successfully constructed by overcrowding,noise and sleep disorders.Semi-quantitative scores,body weight,food intake,white blood cell,red blood cell,hemoglobin,erythrocyte specific volume,inactive time after forced swimming,blood lactic acid and IgG can be used as reference indicators.

Key words: Chronic fatigue syndrome (CFS), Indicators, Rat

中图分类号:

R33Q95-33

饶子亮, 冯学轩, 黄小红, 黎莉斯, 李金凤, 邝少松, 王刚. 慢性疲劳综合征大鼠模型相关指标观测[J]. 实验动物与比较医学, 2018, 38(4): 278-283.

RAO Zi-liang, FENG Xue-xuan, HUANG Xiao-hong, LI Li-si, LI Jin-feng, KUANG Shao-song, WANG Gang. Study on Indicators of Chronic Fatigue Syndrome Model in Rat[J]. Laboratory Animal and Comparative Medicine, 2018, 38(4): 278-283.

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