异丙酚对内毒素血症大鼠血清TNF-α、IL-1β及IL-10的影响

›› 2006, Vol. 26 ›› Issue (2): 98-100.

异丙酚对内毒素血症大鼠血清TNF-α、IL-1β及IL-10的影响

中国医科大学附属口腔医院麻醉科,沈阳 110002

收稿日期:2006-02-08 出版日期:2006-06-25 发布日期:2006-06-25

Protective Effect of Propofol on TNF-α、IL-1β and IL-10 in Endotoxin Shock Rats

Department of Anesthesia, Affiliated stomatology Hospital, China Medical University, Shenyang 110002,China

Received:2006-02-08 Online:2006-06-25 Published:2006-06-25

摘要/Abstract

摘要： 目的观察异丙酚对内毒素血症大鼠血清TNF-α、IL-1β及IL-10作用的影响。方法 24只雄性Wistar大鼠分为三组，内毒素组（L组）、异丙酚+内毒素组（P组）和对照组（C组）。L组股静脉注入内毒素（LPS）8mg/kg，后持续输注生理盐水1ml·kg1·h^-1；P组股静脉注入LPS后静脉注射异丙酚10mg/kg，随后以10mg·kg^-1·h^-1维持；C组静脉注入等量生理盐水。于注射LPS后60、120、180、240min取血，用酶联免疫吸附法（ELISA）测定血清中肿瘤坏死因子（TNF-α）、白细胞介素-1β（IL-1β）及白细胞介素-10（IL-10）含量。结果L组血清中TNF-α、IL-1β及IL-10浓度显著升高（P＜0.01），P组各项指标均明显低于L组（P＜0.01）。结论异丙酚可显著抑制内毒素血症时TNF-α、IL-1β及IL-10的水平，减轻内毒素导致的急性肺损伤。

关键词: 二异丙酚, 内毒素类, 肿瘤坏死因子

Abstract: Objective To investigate the effect of propofol on TNF-α、IL-1β and IL-10 of endot-oxic shock rat. Methods Twenty-four male Wistar rats were randomly assigned to three groups: Control group（Group C）, Endotoxin group（Group L）,and propofol plus Endotoxin group （Group P）. Endotoxin with concentration of 10 mg/kg were injected into the rats of group L through femoral vein；The rats in group P had 20 mg/kg/h of propofol injected intravenously and then received 10 mg/kg/h of endotoxin； The rats in group C had the same amount of normal saline injected. The rats were killed 60，120,180 and 240 minutes after the injection. The levels of TNF-α、IL-1β and IL-10 were measured by ELISA. Results In Group L, the concentrations of TNF-α、IL-β and IL-10 rose significantly （P＜0.001）. In Group P, all the concentrations were significantly lower than that of Group L （P＜0.001）.Conclusion Propofol can significantly decrease the level of TNF-α、IL-β and IL-10 in endotoxic shock, and then prevent acute lung injury caused by endotoxin.

Key words: Propofol, Endotoxin, Tumor necrosis factor（TNF）

张倩, 金玄玉, 何晓帆. 异丙酚对内毒素血症大鼠血清TNF-α、IL-1β及IL-10的影响[J]. , 2006, 26(2): 98-100.

ZHANG Qian, JIN Xuan-Yu, HE Xiao-Fan. Protective Effect of Propofol on TNF-α、IL-1β and IL-10 in Endotoxin Shock Rats[J]. , 2006, 26(2): 98-100.

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