抗原类型对诱导型干燥综合征小鼠建模的影响

doi:10.12300/j.issn.1674-5817.2025.175

实验动物与比较医学 ›› 2026, Vol. 46 ›› Issue (2): 178-190.DOI: 10.12300/j.issn.1674-5817.2025.175

抗原类型对诱导型干燥综合征小鼠建模的影响

荣文双¹^,²^,³, 牛源菲⁴, 刘美婷²^,³, 杨梦园⁵, 崔爽²^,³, 马丽娜⁶, 富尧¹, 王连嵋²^,³()(), 曹俊岭¹^,⁶()()

^1.北京中医药大学东直门医院, 北京 100700
^2.中国中医科学院中药研究所中草药中精神活性物质发现与防控北京市重点实验室, 中药安全用药风险评控与转化重点实验室, 北京 100700
^3.中国中医科学院中药研究所道地药材品质保障与资源持续利用重点实验室, 北京 100700
^4.北京中医药大学中药学院, 北京 102488
^5.中国中医科学院中药资源中心, 北京 100700
^6.北京中医药大学东方医院, 北京 100078

收稿日期:2025-10-24 修回日期:2026-02-05 出版日期:2026-04-25 发布日期:2026-04-20
通讯作者: 王连嵋（1981—），女，博士，副研究员，研究方向：中药毒理与安全用药研究。E-mail： lmwang@icmm.ac.cn。ORCID：0000-0001-5328-6223；
曹俊岭（1972—），男，博士，博士生导师，博士后合作导师，研究方向：中药合理使用研究。E-mail： caojunling72@163.com。ORCID：0000-0001-7640-8713
作者简介:荣文双（1996—），女，硕士研究生，研究方向：中医优势病种的中医药治疗。E-mail： rongwenshuang123@163.com
基金资助:
国家中医药管理局项目“特色饮片品种挖掘应用-柴胡”(GZY-KJ5-2022-057);中国中医科学院中药研究所中央级公益性科研院所基本科研业务费专项资金资助项目“中药材拟境栽培与品质保障重点实验室”(ZXKT25043);“中国中医科学院中药安全用药风险评控与转化重点实验室”(ZXKT25036)

Influence of Antigen Type on the Establishment of an Induced Sjögren Syndrome Mouse Model

RONG Wenshuang¹^,²^,³, NIU Yuanfei⁴, LIU Meiting²^,³, YANG Mengyuan⁵, CUI Shuang²^,³, MA Lina⁶, FU Yao¹, WANG Lianmei²^,³()(), CAO Junling¹^,⁶()()

^1.Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
^2.Beijing Key Laboratory of Psychoactive Substances Discovery and Control in Chinese Herbal Medicines, Key Laboratory of TCM Safety Risk Assessment and Translation, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
^3.State Key Laboratory for Quality Assurance and Sustainable Use of Dao-di Herbs, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
^4.School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China
^5.National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
^6.Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China

Received:2025-10-24 Revised:2026-02-05 Published:2026-04-25 Online:2026-04-20
Correspondence to: CAO Junling (ORCID: 0000-0001-7640-8713), E-mail: caojunling72@163.com;
WANG Lianmei (ORCID: 0000-0001-5328-6223), E-mail: lmwang@icmm.ac.cn

摘要/Abstract

摘要：

目的观察比较颌下腺抗原和唾液腺抗原用于诱导干燥综合征（Sj?gren syndrome，SS）小鼠模型的建模效果，并与自发性SS模型NOD/LtJ小鼠进行比较，明确两种腺体抗原诱导的SS小鼠模型的表型与免疫特征。方法选用成年C57BL/6J小鼠（雌雄各半），分别采用颌下腺抗原和唾液腺抗原联合弗氏佐剂免疫小鼠，诱导建立SS模型；以PBS联合弗氏佐剂免疫小鼠作为对照。在第1天和第7天时采用抗原联合弗氏完全佐剂（Freund’s complete adjuvant，FCA）经小鼠背部皮下多点注射进行免疫诱导，第14天采用抗原联合弗氏不完全佐剂（Freund’s incomplete adjuvant，FIA）经小鼠背部皮下多点注射进行加强免疫诱导。同时设置雌性NOD/LtJ小鼠为自发性SS模型组，以其背景品系ICR小鼠为对照，进行对比分析。实验周期为4周，动态监测小鼠体重、饮水量及唾液流量的变化。在实验终点时收集小鼠各脏器组织与血清样本，称量颌下腺、胸腺和脾脏重量，计算脏器指数；对颌下腺和脾脏组织进行病理形态学分析；采用ELISA法检测血清中白细胞介素-17（interleukin-17，IL-17）含量；采用实时荧光定量PCR法检测颌下腺组织中SS抗原A（SS type A，SSA）和SS抗原B（SS type B，SSB）的mRNA表达水平。结果与雌性的PBS联合弗氏佐剂对照组相比，颌下腺抗原雌性组小鼠的饮水量明显增加（P＜0.05），唾液分泌量明显减少（P＜0.05），颌下腺指数、胸腺指数和脾脏指数均无明显差异（P＞0.05），颌下腺出现灶性淋巴细胞浸润、脾脏边缘区扩大，血清IL-17水平明显升高（P<0.05），颌下腺组织中SSA/SSB表达水平无明显差异（P＞0.05）。与雌性的PBS联合弗氏佐剂对照组相比，唾液腺抗原雌性组小鼠饮水量、唾液分泌量、颌下腺指数以及脾脏指数均无明显差异（P＞0.05），但胸腺指数明显降低（P＜0.01）；颌下腺出现轻微炎性细胞浸润和腺体萎缩，脾脏白髓及边缘区略有扩大；血清IL-17水平与颌下腺组织中SSB表达水平均明显升高（P＜0.01），颌下腺组织中SSA表达水平无明显变化（P＞0.05）。与雄性的PBS联合弗氏佐剂对照组相比，除颌下腺抗原雄性组小鼠出现轻微颌下腺腺体萎缩外，各造模组的其余成模指标均没有明显差异（P＞0.05）。与ICR对照组相比，NOD/LtJ模型组小鼠饮水量明显增加（P＜0.05），唾液流量明显减少（P＜0.01），颌下腺指数和脾脏指数均无明显差异（P＞0.05），但胸腺指数明显增大（P＜0.05）；颌下腺病理灶性浸润明显，脾脏边缘区明显扩大，血清IL-17水平和颌下腺SSA/SSB表达水平均明显升高（P＜0.05）。结论颌下腺抗原和唾液腺抗原均能诱导雌性C57BL/6J小鼠表现出SS相关特征，而颌下腺抗原诱导的SS相关表型较唾液腺抗原诱导更为明显，但弱于自发性雌性NOD/LtJ小鼠。用颌下腺抗原和唾液腺抗原接种雄性C57BL/6J小鼠未能诱导出明显的SS表型。

关键词: 干燥综合征, 颌下腺抗原, 唾液腺抗原, C57BL/6J 小鼠, NOD/LtJ小鼠

Abstract:

Objective This study aims to compare the modeling effects of submaxillary gland antigen and salivary gland antigen in the establishment of Sj?gren syndrome (SS) mouse models, and to characterize the phenotypic and immunological features of these models in comparison with spontaneous SS-prone non-obese diabetic (NOD)/LtJ mice. Methods Adult C57BL/6J mice (equal numbers of males and females) were immunized with submaxillary gland antigen or salivary gland antigen, respectively, combined with Freund's adjuvant to induce SS models. Mice immunized with phosphate-buffered saline (PBS) combined with Freund's adjuvant served as the control group. Immunization was induced via multiple subcutaneous injections in the back with antigen combined with Freund's complete adjuvant (FCA) on Days 1 and 7. A booster immunization was administered via multiple subcutaneous injections in the back with antigen combined with Freund's incomplete adjuvant (FIA) on Day 14. Female NOD/LtJ mice were used as the spontaneous SS model group, with ICR mice as the corresponding control strain for comparative analysis. Body weight, water intake, and salivary flow rate of mice were dynamically monitored for 4 weeks. At the end of the experiment, tissue and serum samples were collected, the weights of submaxillary glands, thymus, and spleen were measured, and organ indices (organ-to-body weight ratios) were calculated. Pathological morphological analysis of the submaxillary gland and spleen was performed with hematoxylin and eosin (HE) staining. Serum interleukin-17 (IL-17) level was detected using enzyme-linked immunosorbent assay (ELISA). Real-time quantitative polymerase chain reaction was used to detect the mRNA expression levels of SS type A (SSA) and SS type B (SSB) in submaxillary gland tissues. Results Female mice in the submaxillary gland antigen group exhibited significantly increased water intake (P<0.05) and reduced salivary flow rate (P<0.05) compared with the female control group. No statistically significant differences were observed in the submaxillary gland index, thymus index and spleen index (P>0.05). Focal lymphocytic infiltration was observed in the submaxillary glands, and the splenic marginal zone was enlarged. Serum IL-17 levels were significantly increased (P<0.05). There was no significant difference in submaxillary gland SSA/SSB expression levels (P>0.05). Compared with the female control group, female mice in the salivary gland antigen group showed no statistically significant differences in water intake, salivary flow rate, submaxillary gland index, and spleen index (P>0.05), whereas the thymus index was significantly reduced (P<0.01). Mild inflammatory cell infiltration and glandular atrophy were observed in the submaxillary glands, and the splenic white pulp and marginal zone were slightly enlarged. Serum IL-17 levels and submaxillary gland SSB mRNA expression levels were significantly increased (P<0.01), whereas no significant change was observed in submaxillary gland SSA expression levels (P>0.05). Compared with the male control group, mild submaxillary gland atrophy was observed in male mice in the submaxillary gland antigen group, whereas no obvious changes were found in other modeling-related indicators (P>0.05). Compared with the ICR control group, NOD/LtJ model mice exhibited elevated water intake (P<0.05), significantly reduced salivary flow rate (P<0.01), no significant differences in the submaxillary gland index or spleen index (P>0.05), but a significantly increased thymus index (P<0.05). Marked focal infiltration was observed in the submaxillary glands, the splenic marginal zone was obviously enlarged, and serum IL-17 concentrations as well as submaxillary gland SSA/SSB expression levels were significantly increased (P<0.05). Conclusion Submaxillary gland antigen and salivary gland antigen can induce SS-related features in female C57BL/6J mice. The SS-related phenotype is more pronounced in the submaxillary gland antigen group than in the salivary gland antigen group, but weaker than that in spontaneously SS-prone female NOD/LtJ mice. Immunization of male C57BL/6J mice with submaxillary or salivary gland antigens fails to induce an obvious SS phenotype.

Key words: Sj?gren syndrome, Submaxillary gland antigen, Salivary gland antigen, C57BL/6J mice, NOD/LtJ mice

中图分类号:

Q95-33
R-332

荣文双,牛源菲,刘美婷,等. 抗原类型对诱导型干燥综合征小鼠建模的影响[J]. 实验动物与比较医学, 2026, 46(2): 178-190. DOI: 10.12300/j.issn.1674-5817.2025.175.

RONG Wenshuang,NIU Yuanfei,LIU Meiting,et al. Influence of Antigen Type on the Establishment of an Induced Sjögren Syndrome Mouse Model[J]. Laboratory Animal and Comparative Medicine, 2026, 46(2): 178-190. DOI: 10.12300/j.issn.1674-5817.2025.175.

图/表 7

表1 PCR 引物序列

Table 1 PCR primer sequences

引物名称 Primer name	引物序列 Primer sequence
m-SSA-F	5'-GCTGGTCTCACAAAGATCTCCTCC-3'
m-SSA-R	5'-TCTCAGCCTCCACAGAAAGCG-3'
m-SSB-F	5'-ATGACTGCTTTGGAGGCCAAAATC-3'
m-SSB-R	5'-TGTCAGCCGGTTTAGCCTGTTGAA-3'
m-β-Actin-F	5'-AGAGGGAAATCGTGCGTGAC-3'
m-β-Actin-R	5'-CAATAGTGATGACCTGGCCGT-3'

注：A，各组雌性C57BL/6J小鼠的体重。B，各组雌性C57BL/6J小鼠的饮水量。C，各组雌性C57BL/6J小鼠颌下腺组织中干燥综合征抗原A（SSA）和干燥综合征抗原B（SSB）的相对表达量。D，各组雌性C57BL/6J小鼠血清中白细胞介素-17（IL-17）的含量。E，各组雌性C57BL/6J小鼠颌下腺HE染色结果，比例尺为50 μm和20 μm（局部放大）。F，各组雌性C57BL/6J小鼠脾脏HE染色结果，比例尺为100 μm。♀Control表示PBS联合弗氏佐剂免疫雌性对照组，♀salivary gland表示唾液腺抗原联合弗氏佐剂免疫雌性组，♀submaxillary gland表示颌下腺抗原联合弗氏佐剂免疫雌性组，每组8只。与♀control相比，^*P＜0.05，^**P＜0.01，^***P＜0.001。
">

图 1 免疫诱导后雌性C57BL/6J小鼠的干燥综合征相关表型
注：A，各组雌性C57BL/6J小鼠的体重。B，各组雌性C57BL/6J小鼠的饮水量。C，各组雌性C57BL/6J小鼠颌下腺组织中干燥综合征抗原A（SSA）和干燥综合征抗原B（SSB）的相对表达量。D，各组雌性C57BL/6J小鼠血清中白细胞介素-17（IL-17）的含量。E，各组雌性C57BL/6J小鼠颌下腺HE染色结果，比例尺为50 μm和20 μm（局部放大）。F，各组雌性C57BL/6J小鼠脾脏HE染色结果，比例尺为100 μm。♀Control表示PBS联合弗氏佐剂免疫雌性对照组，♀salivary gland表示唾液腺抗原联合弗氏佐剂免疫雌性组，♀submaxillary gland表示颌下腺抗原联合弗氏佐剂免疫雌性组，每组8只。与♀control相比，^*P＜0.05，^**P＜0.01，^***P＜0.001。

Figure 1 Sj?gren syndrome-related phenotypes in antigen-induced female C57BL/6J mice
Note： A， Body weight of female C57BL/6J mice in each group. B， Water intake of female C57BL/6J mice in each group. C， Relative expression levels of Sj?gren syndrome antigen A （SSA） and Sj?gren syndrome antigen B （SSB） in the submaxillary gland tissues of female C57BL/6J mice in each group. D， Serum interleukin-17 （IL-17） concentration in female C57BL/6J mice in each group. E， HE staining of the submaxillary gland in female C57BL/6J mice in each group， scale bar= 50 μm （partial magnification scale size=20 μm）. F， HE staining of the spleen in female C57BL/6J mice in each group， scale bar= 100 μm. ♀Control， Female mice immunized with phosphate-buffered saline （PBS） combined with Freund's adjuvant served as control group； ♀Salivary gland， Female mice immunized with salivary gland antigen combined with Freund's adjuvant； ♀Submaxillary gland， Female mice immunized with submaxillary gland antigen combined with Freund's adjuvant. n=8. Compared with ♀ control group， ^*P<0.05， ^**P＜0.01， ^***P＜0.001.

表2 雌性C57BL/6J各组小鼠唾液流量和脏器指数 (

x ˉ

±s, n=8)

Table 2 Salivary flow rate and organ indices in female C57BL/6J mice of each group

xˉ±s, n=8)
">

组别

Group

第0天唾液流量/

（mg·min^-1）

Day 0 salivary flow rate /(mg·min^-1)

第30天唾液流量/

(mg·min^-1)

Day 30 salivary flow rate /(mg·min^-1)

颌下腺指数

Submaxillary gland index

胸腺指数

Thymus index

脾指数

Spleen index

PBS-佐剂雌性对照组

PBS-adjuvanted female control group

唾液腺抗原雌性组

Salivary gland antigen female group

颌下腺抗原雌性组

Submaxillary gland antigen female group

表2 雌性C57BL/6J各组小鼠唾液流量和脏器指数 ( $x ˉ$ ±s, n=8)

Table 2 Salivary flow rate and organ indices in female C57BL/6J mice of each group

组别

Group

第0天唾液流量/

（mg·min^-1）

Day 0 salivary flow rate /(mg·min^-1)

第30天唾液流量/

(mg·min^-1)

Day 30 salivary flow rate /(mg·min^-1)

颌下腺指数

Submaxillary gland index

胸腺指数

Thymus index

脾指数

Spleen index

PBS-佐剂雌性对照组

PBS-adjuvanted female control group

6.60±1.11

14.77±1.04

0.28±0.03

0.26±0.04

0.44±0.04

唾液腺抗原雌性组

Salivary gland antigen female group

6.28±1.10

13.10±1.05

0.29±0.03

0.20±0.02^**

0.51±0.13

颌下腺抗原雌性组

Submaxillary gland antigen female group

6.14±1.16

10.52±1.06^*

0.30±0.03

0.24±0.04

0.50±0.06

表3 雄性C57BL/6J各组小鼠的唾液流量和脏器指数 (

x ˉ

±s, n=8)

Table 3 Salivary flow rate and organ indices in male C57BL/6J mice of each group

xˉ±s, n=8)
">

组别

Group

第0天唾液流量/

（mg·min^-1）

Day 0 salivary flow rate /（mg·min^-1）

第30天唾液流量/（mg·min^-1）

Day 30 salivary flow rate /（mg·min^-1）

颌下腺指数

Submaxillary gland index

胸腺指数

Thymus index

脾指数

Spleen index

PBS-佐剂雄性对照组

PBS-adjuvanted male control group

唾液腺抗原雄性组

Salivary gland antigen male group

颌下腺抗原雄性组

Submaxillary gland antigen male group

表3 雄性C57BL/6J各组小鼠的唾液流量和脏器指数 ( $x ˉ$ ±s, n=8)

Table 3 Salivary flow rate and organ indices in male C57BL/6J mice of each group

组别

Group

第0天唾液流量/

（mg·min^-1）

Day 0 salivary flow rate /（mg·min^-1）

第30天唾液流量/（mg·min^-1）

Day 30 salivary flow rate /（mg·min^-1）

颌下腺指数

Submaxillary gland index

胸腺指数

Thymus index

脾指数

Spleen index

PBS-佐剂雄性对照组

PBS-adjuvanted male control group

6.21±0.88

14.60±0.90

0.38±0.03

0.18±0.06

0.41±0.07

唾液腺抗原雄性组

Salivary gland antigen male group

6.72±0.73

11.17±0.62

0.40±0.04

0.18±0.05

0.37±0.14

颌下腺抗原雄性组

Submaxillary gland antigen male group

6.17±0.90

11.54±0.70

0.38±0.06

0.17±0.03

0.34±0.05

注：A，各组雄性C57BL/6J小鼠的体重；B，各组雄性C57BL/6J小鼠的饮水量；C，各组雄性C57BL/6J小鼠颌下腺组织中干燥综合征抗原A（SSA）和干燥综合征抗原B（SSB）的表达量；D，各组雄性C57BL/6J小鼠血清白细胞介素-17（IL-17）的含量；E，各组雄性C57BL/6J小鼠颌下腺HE染色结果，标尺为50 μm；F，各组雄性C57BL/6J小鼠脾脏HE染色结果，标尺为100 μm。♂Control表示PBS联合弗氏佐剂免疫雄性对照组，♂salivary gland表示唾液腺抗原联合弗氏佐剂免疫雄性组，♂submaxillary gland表示颌下腺抗原联合弗氏佐剂免疫雄性组，每组8只。与♂control相比， ^#P＜0.05。
">

图 2 免疫诱导后雄性C57BL/6J小鼠的干燥综合征相关表型
注：A，各组雄性C57BL/6J小鼠的体重；B，各组雄性C57BL/6J小鼠的饮水量；C，各组雄性C57BL/6J小鼠颌下腺组织中干燥综合征抗原A（SSA）和干燥综合征抗原B（SSB）的表达量；D，各组雄性C57BL/6J小鼠血清白细胞介素-17（IL-17）的含量；E，各组雄性C57BL/6J小鼠颌下腺HE染色结果，标尺为50 μm；F，各组雄性C57BL/6J小鼠脾脏HE染色结果，标尺为100 μm。♂Control表示PBS联合弗氏佐剂免疫雄性对照组，♂salivary gland表示唾液腺抗原联合弗氏佐剂免疫雄性组，♂submaxillary gland表示颌下腺抗原联合弗氏佐剂免疫雄性组，每组8只。与♂control相比， ^#P＜0.05。

Figure 2 Sj?gren syndrome-related phenotypes in antigen-induced male C57BL/6J mice
Note： A，Body weight of male C57BL/6J mice in each group； B， Water intake of male C57BL/6J mice in each group； C， Relative expression levels of Sj?gren syndrome antigen A （SSA） and Sj?gren syndrome antigen B （SSB） in the submaxillary gland tissues of male C57BL/6J mice in each group； D， Serum interleukin-17 （IL-17） concentration in male C57BL/6J mice in each group； E， HE staining of the submaxillary gland in male C57BL/6J mice in each group （Scale bar=50 μm）； F， HE staining of the spleen in male C57BL/6J mice in each group （Scale bar=100 μm）. ♂Control， Male mice immunized with phosphate-buffered saline （PBS） combined with Freund's adjuvant served as control group； ♂Salivary gland， Male mice immunized with salivary antigen combined with Freund's adjuvant； ♂Submaxillary gland， Male mice immunized with submaxillary gland antigen combined with Freund's adjuvant. n=8. Compared with ♂control group， ^#P＜0.05.

注：A，ICR小鼠和NOD/LtJ模型小鼠的体重变化；B，ICR小鼠和NOD/LtJ模型小鼠的饮水量变化；C，ICR小鼠和NOD/LtJ模型小鼠颌下腺组织中干燥综合征抗原A（SSA）和干燥综合征抗原B（SSB）相对表达量；D，ICR小鼠和NOD/LtJ模型小鼠血清白细胞介素-17（IL-17）含量；E，ICR小鼠和NOD/LtJ模型小鼠的颌下腺HE染色结果，标尺为50 μm和20 μm（局部放大）；F，ICR小鼠和NOD/LtJ模型小鼠的脾脏HE染色结果，标尺为100 μm。NOD/LtJ model表示NOD/LtJ小鼠自发干燥综合征模型组，ICR control表示ICR小鼠对照组，n=8。与ICR对照组相比，^*P<0.05，^**P＜0.01，^***P＜0.001，^****P<0.000 1。
">

图 3 雌性NOD/LtJ模型小鼠与 ICR小鼠的干燥综合征相关表型
注：A，ICR小鼠和NOD/LtJ模型小鼠的体重变化；B，ICR小鼠和NOD/LtJ模型小鼠的饮水量变化；C，ICR小鼠和NOD/LtJ模型小鼠颌下腺组织中干燥综合征抗原A（SSA）和干燥综合征抗原B（SSB）相对表达量；D，ICR小鼠和NOD/LtJ模型小鼠血清白细胞介素-17（IL-17）含量；E，ICR小鼠和NOD/LtJ模型小鼠的颌下腺HE染色结果，标尺为50 μm和20 μm（局部放大）；F，ICR小鼠和NOD/LtJ模型小鼠的脾脏HE染色结果，标尺为100 μm。NOD/LtJ model表示NOD/LtJ小鼠自发干燥综合征模型组，ICR control表示ICR小鼠对照组，n=8。与ICR对照组相比，^*P<0.05，^**P＜0.01，^***P＜0.001，^****P<0.000 1。

Figure 3 Sj?gren syndrome-related phenotypes in female NOD/LtJ model mice and ICR mice
Note： A， Body weight changes in ICR and NOD/LtJ model mice； B， Water intake changes in ICR and NOD/LtJ model mice； C， Relative expression levels of Sj?gren syndrome antigen A （SSA） and Sj?gren syndrome antigen B （SSB） in the submaxillary gland tissues of ICR and NOD/LtJ model mice； D， Serum interleukin-17 （IL-17） concentration in ICR and NOD/LtJ model mice； E， HE staining of the submaxillary gland in ICR and NOD/LtJ model mice， scale bar=50 μm （partial magnification scale size=20 μm）； F， HE staining of the spleen in ICR and NOD/LtJ model mice， scale bar=100 μm. ICR control， ICR control group； NOD/LtJ model， NOD/LtJ model group. NOD/LtJ mice were used as a spontaneous Sj?gren syndrome model group， with ICR mice as the corresponding control strain for comparative analysis. n=8. Compared with ICR control group， ^*P<0.05， ^**P＜0.01， ^***P＜0.001， ^****P<0.000 1.

表4 雌性NOD/LtJ模型小鼠与ICR小鼠的唾液流量和脏器指数比较 (

x ˉ

±s, n=8)

Table 4 Salivary flow rate and organ indices in female NOD/LtJ model mice and ICR mice

xˉ±s, n=8)
">

组别

Group

第0天唾液流量/（mg·min^-1）

Day 0 salivary flow rate /（mg·min^-1）

第30天唾液流量/（mg·min^-1）

Day 30 salivary flow rate /

（mg·min^-1）

颌下腺指数

Submaxillary gland index

胸腺指数

Thymus index

脾指数

Spleen index

表4 雌性NOD/LtJ模型小鼠与ICR小鼠的唾液流量和脏器指数比较 ( $x ˉ$ ±s, n=8)

Table 4 Salivary flow rate and organ indices in female NOD/LtJ model mice and ICR mice

组别

Group

第0天唾液流量/（mg·min^-1）

Day 0 salivary flow rate /（mg·min^-1）

第30天唾液流量/（mg·min^-1）

Day 30 salivary flow rate /

（mg·min^-1）

颌下腺指数

Submaxillary gland index

胸腺指数

Thymus index

脾指数

Spleen index

ICR mice

8.37±0.46

15.85±1.25

0.39±0.01

0.13±0.03

0.39±0.08

NOD/LtJ mice

6.42±0.05^*

5.11±0.46^**

0.40±0.04

0.16±0.03^*

0.31±0.05

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抗原类型对诱导型干燥综合征小鼠建模的影响

Influence of Antigen Type on the Establishment of an Induced Sjögren Syndrome Mouse Model

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