SPF鸡不同生长阶段粪便菌群组成及多样性研究

doi:10.3969/j.issn.1674-5817.2017.03.013

实验动物与比较医学 ›› 2017, Vol. 37 ›› Issue (3): 231-239.DOI: 10.3969/j.issn.1674-5817.2017.03.013

所属专题：实验动物资源开发与利用

• 禽类实验动物的研究与应用 • 上一篇下一篇

SPF鸡不同生长阶段粪便菌群组成及多样性研究

周妍^1,2, 刁晨曦^1,3, 张圆圆¹, 于海波¹, 陆涛峰¹, 赵丽丽¹, 陈洪岩¹

1.中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/黑龙江省实验动物与比较医学重点实验室, 实验动物与比较医学创新团队, 哈尔滨 150069;
2.东北农业大学生命科学学院, 哈尔滨 150030;
3.牡丹江师范学院生命科学与技术学院, 牡丹江157011

收稿日期:2017-03-16 出版日期:2017-06-25 发布日期:2017-06-25
作者简介:周妍(1993-),女,硕士。研究方向:实验动物与比较医学。E-mail:2609814396@qq.com;共同第一作者:刁晨曦(1992-),女,硕士。研究方向:实验动物与比较医学。E-mail:2410880274@qq.com
基金资助:
国家科技支撑计划(2015BAI07B02-02);中国农业科学院基本科研业务费专项(Y2016PT41);国家自然科学基金(31601974)

Composition and Diversity of Fecal Microflora in SPF Chickens at Different Growth Stages

ZHOU Yan^1,2, DIAO Chen-xi^1,3, ZHANG Yuan-yuan¹, YU Hai-bo¹, LU Tao-feng¹, ZHAO Li-li¹, CHEN Hong-yan¹

1. Division of Laboratory Animal and Comparative Medicine, State Key Laboratory of Veterinary Biotechnology/Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences, Harbin 150069, China;
2. College of Life Science, Northeast Agricultural University, Harbin 150030, China;
3. College of Life Science and Technology, Mudanjiang Normal University, Mu Danjiang 157011, China

Received:2017-03-16 Online:2017-06-25 Published:2017-06-25

摘要/Abstract

摘要： 目的提供SPF鸡不同生长阶段肠道菌群的组成及多样性数据。方法采集10日龄、12周龄、23周龄和52周龄SPF鸡粪便,利用Illumina HiSeq 2500高通量测序方法,对样品16S rRNA的两个高变区(V3-V4)进行测序分析,并进行了〈-多样性指数、α-多样性指数和粪便群落特征分析。结果四个生长阶段粪便样品菌群具有相似的较高的丰富度和良好的均匀度,物种多样性无显著差异(P>0.05); 厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)和拟杆菌门(Bacteroidetes)丰度占95%以上; 乳杆菌属(Lactobacillus)、链球菌属(Streptococcus)、拟杆菌属(Bacteroides)、肠球菌属(Enterococcus)为优势菌属,不同时期丰度差异达显著水平(P<0.05)。结论四个生长阶段菌群组成多样,且优势菌群主要为有益菌。该实验结果能够为SPF鸡品质评定、健康监测和安全性评价提供依据。

关键词: SPF鸡, 不同生长阶段, 粪便菌群, 16s rRNA, Illumina HiSeq 2500高通量测序

Abstract: Objective To provide the composition and diversity of intestinal flora at different growth stages of SPF chickens. Methods The feces of SPF chickens at 10 d , 12 weeks , 23 weeks and 52 weeks old were collected. The two hypervariable regions (V3-V4) of the 16S rRNA of the samples were sequenced using the Illumina HiSeq 2500 platform, and the alpha diversity, beta diversity and fecal community characteristics were analyzed. Results The fecal flora sample at four time points had similar high abundance and good uniformity and there was no significant difference in species diversity between the two groups (P>0.05). More than 95% of abundance were found in Firmicutes, Proteobacteria and Bacteroidetes; Lactobacillus, Streptococcus, Bacteroides, and Enterococcus were dominant species, and the abundance of different periods were significantly different (P<0.05). Conclusions The feces flora of SPF chiken at different growth stages are diverse, and the dominant bacteria are mainly beneficial bacteria. The experimental results are expected to provide the basis for assessing the quality, health monitoring and safety evaluation of SPF chickens.

Key words: SPF chicken, Different growth stages, Feces microbiota, 16s rRNA, Illumina HiSeq 2500 platform

中图分类号:

Q95-33

周妍, 刁晨曦, 张圆圆, 于海波, 陆涛峰, 赵丽丽, 陈洪岩. SPF鸡不同生长阶段粪便菌群组成及多样性研究[J]. 实验动物与比较医学, 2017, 37(3): 231-239.

ZHOU Yan, DIAO Chen-xi, ZHANG Yuan-yuan, YU Hai-bo, LU Tao-feng, ZHAO Li-li, CHEN Hong-yan. Composition and Diversity of Fecal Microflora in SPF Chickens at Different Growth Stages[J]. Laboratory Animal and Comparative Medicine, 2017, 37(3): 231-239.

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SPF鸡不同生长阶段粪便菌群组成及多样性研究

Composition and Diversity of Fecal Microflora in SPF Chickens at Different Growth Stages

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