›› 2009, Vol. 29 ›› Issue (6): 411-414.

所属专题: 实验动物资源开发与利用

• 论文 • 上一篇    下一篇

WHBE兔网织红细胞计数与分群测定

  

  1. 浙江中医药大学动物实验研究中心/比较医学研究中心,杭州,310053
  • 收稿日期:2009-03-17 出版日期:2009-06-30 发布日期:2009-06-30
  • 基金资助:
    1.浙江省公共科技基础平台项目(Dwpt-bz-12);2.浙江省卫生高层次创新人才培养工程项目

Measurement of Reticulocytes and Its Subpopulation in WHBE Rabbits

  1. Zhejiang Chinese Medical University Laboratory Animal Research Center, Hangzhou 310053, China
  • Received:2009-03-17 Online:2009-06-30 Published:2009-06-30

摘要: 目的 测定和比较分析WHBE兔、日本大耳白兔和新西兰白兔的网织红细胞计数与分群的变化。方法 选择WHBE兔、日本大耳白兔、新西兰白兔各40只,雌雄各半,耳动脉采血,应用Bayer ADVIA 120五分类血球分析仪测定实验兔网织红细胞参数。结果 ①性别因素对WHBE兔RDwr,HDWr、CHDWr、RDWm、CHDWm、RDWg、CHDWg等有显著差异(P<0.05,P<0.01);②WHBE兔的网织红细胞参数与日本大耳白兔和新西兰白兔存在着显著差异,如WHBE兔的网织红细胞参数均明显低于日本大耳白兔、新西兰白兔(P<0.01);③与人的血液学生理指标比较,发现WHBE兔的网织红细胞生成指数与人类接近。结论 WHBE兔网织红细胞计数与分群有其自身的特性,有可能作为人类贫血疾病等研究的模型动物。

关键词: WHBE兔, 日本大耳白兔, 新西兰兔, 网织红细胞

Abstract: Objective To determine and comparative analysis of reticulocytes and its subpopulation in WHBE rabbits,Japanese White rabbits and New Zealand White rabbits. Methods Fourty in each of WHBE rabbits, Japanese White rabbits and New Zealand White rabbits, half male and female, were used. The anticoagulant blood samples from the central artery of ear, and then reticulocytes parameters indexes were determined by Bayer ADVIA 120 automatic blood analyzer. Results (1) Gender factor has significant influences on RDWr,HDWr,CHDWr,RDWm, CHDWm, RDWg, CHDWg indexes m WHBE rabbits (P<0.05,P<0.01); (2) Reticulocytes parameters in WHBE rabbits had significant differences between Japanese White rabbits and New Zealand White rabbits, for example, Retie % was lower in WHBE rabbits than that of Japanese White rabbits and New Zealand White rabbits(P<0.01); (3)RPI in WHBE rabbits was similar to that of human being, compared with human reference. Conclusion WHBE rabbit has its own reticulocytes and its subpopulation characteristics, and may be used as model animal for research on anemia disease etc. in human.

Key words: WHBE rabbit, Japanese White rabbit, New Zealand White rabbit, Reticulocyte parameter