猪骨髓间质干细胞体外分离,培养鉴定及向肌细胞转化的探索

›› 2003, Vol. 23 ›› Issue (2): 67-69.

猪骨髓间质干细胞体外分离,培养鉴定及向肌细胞转化的探索

1.上海第二医科大学附属仁济医院心胸外科,上海 200001；2.江苏大学医疗技术学院临床检验教研室,镇江 212001；3.上海第二医科大学附属新华医院心胸外科,上海 200092；4.上海第二医科大学病理教研室,上海 200025

收稿日期:2002-07-15 出版日期:2003-01-25 发布日期:2013-03-19
基金资助:
国家自然科学基金资助项目（30070748）

Isolation, Culture and Identification of Porcine Bone Marrow Mesenchymal Stem Cells and Transformation to Myogenic Cells in vitro

1.Department of Cardio-T horacic Surgery, Renji Hospital，Shanghai Second Medical University， Shanghai 200001，China；2.Department of Clinical Laboratory, Collage of Technique, Jiangsu University，Zhenjiang 212001，China；3.Department of Cardio-Thoracic Surgery，Xinhua Hospital,shanghai Second Medical University，Shanghai 200082，China；4.Department of Pathology，Shanghai Second Medical University，Shanghai 200025，China

Received:2002-07-15 Online:2003-01-25 Published:2013-03-19

摘要/Abstract

摘要： 为建立一种稳定的体外分离，培养及鉴定自体骨髓间质干细胞的方法，抽取贵州香猪骨髓液3ml，以1×10⁶细胞浓度培养，2d后得到贴壁生长的细胞，约10d后进行传代培养，传代周期约为5～7d。选原代及10次传代后的贴壁细胞向成骨细胞及肌细胞转化。原代及传代培养的贴壁细胞为纺锤形细胞。两种细胞在体外特殊诱导环境下可定向分化为成骨细胞及肌细胞。通过本方法培养得到的细胞经长期传代后仍具有多能转化活性，确系骨髓间质干细胞。

关键词: 骨髓间质干细胞, 细胞培养, 肌细胞, 成骨细胞

Abstract: To establish a method of isolating and obtaining porcine bone marrow mesenchymal stem cells （MSCs）in vitro，and to assess the possibility of MSCs as a new ccll source for tissue engineering. Three ml bone marrow was extracted from the posterior superior iliac spine of the mini-pig and culturd by 1×10⁶/ml cells. Some cells were attached to the culture dish after the second day.The adherent cells approached confluence 10 days after plating and then every passage was cultured for 5～7 days. The primary culture and 10th passaged adherent cells were induced to differentiate into osteoblast and myogenic cells. The primary culture and the passaged cells were spindle-shaped in appearance.They both might be induced to differentiate into bone marrow mesenchymal cells and osteoblast and myogenic cells under special environment. The method for isolating and culturing porcine MSCs was feasible.

Key words: Bone marrow mesenchymal stem cells, Cell culture, Ostoblast, Myogenic cells

连锋1，朱洪生1，朱伟2，钟竑3，郑林4. 猪骨髓间质干细胞体外分离,培养鉴定及向肌细胞转化的探索[J]. , 2003, 23(2): 67-69.

LIAN Feng1, ZHU Hong-sheng1, ZHU Wei2, ZHONG Hong3,ZHEN Lin4. Isolation, Culture and Identification of Porcine Bone Marrow Mesenchymal Stem Cells and Transformation to Myogenic Cells in vitro[J]. , 2003, 23(2): 67-69.

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