益骨汤含药血清对新生大鼠成骨细胞增殖及分泌IL-6的影响

doi:10.3969/j.issn.1674-5817.2012.02.014

实验动物与比较医学 ›› 2012, Vol. 32 ›› Issue (2): 146-148.DOI: 10.3969/j.issn.1674-5817.2012.02.014

益骨汤含药血清对新生大鼠成骨细胞增殖及分泌IL-6的影响

陈芳¹, 朱以良¹, 杨李¹, 唐瑛²

1.广州军区武汉总医院医学实验科, 武汉 430070;
2.海军医学研究所, 上海 200433

收稿日期:2011-06-10 出版日期:2012-04-25 发布日期:2012-04-25
作者简介:陈芳(1979-),女,硕士,药师。研究方向:中药药理学及细胞生物学。E-mail:wpcf401717@163.com
基金资助:
广州军区武汉总医院课题(编号: 200916)

Effect of YIGUTANG Containing Serum on Osteoblast Proliferation and IL-6 Secretion

CHEN Fang¹, ZHU Yi-liang¹, YANG Li¹, TANG Ying²

1. Department of Laboratory, Wuhan General Hospital, Guangzhou Military Region, Wuhan 430070, China;
2. Navy Medical Research Institute, Shanghai 200433, China

Received:2011-06-10 Online:2012-04-25 Published:2012-04-25

摘要/Abstract

摘要： 目的探讨益骨汤含药血清对新生大鼠成骨细胞增殖及分泌IL-6的影响。方法采用酶消化法获得新生大鼠的成骨细胞,然后以益骨汤含药血清作用于成骨细胞,用MTT法检测成骨细胞增殖,ELISA法测定培养上清中IL-6的含量。结果益骨汤各剂量组 (A、B、C组)能促进成骨细胞的增殖,能使其分泌IL-6的能力下降,与空白对照组(E组)比较有显著性差异(P <0.05)。结论益骨汤含药血清能够促进成骨细胞的增殖,同时抑制成骨细胞分泌IL-6。

关键词: 益骨汤含药血清, 大鼠成骨细胞, 细胞增殖, 白介素-6(IL-6)

Abstract: Objective To research the effect of YIGUTANG containing serum on osteoblast proliferation which was from the skull of new born Wistar rat in vitro, and IL-6 secretion. Methods Iliac bone specimens were obtained from the newborn rat calvaria, after that the bone pieces were digested by collagenase-trypsin. The proliferation of osteoblast was observed using MTT reduction assay. The IL-6 in the supernatant was detected by ELISA. Results The proliferation of osteoblast could be stimulated by the serum with different dosages of absorbed YIGUTANG (group A 、B 、C). Meanwhile, the levels of IL-6 secretion in group A, B and C could be significantly decreased compared with the control group (P<0.05). Conclusion The proliferation of osteoblast can be stimulated by the serum with absorbed YIGUTANG and the capability of producing IL-6 in osteoblast also can be restrained by the serum.

Key words: YIGUTANG containing serum, Osteoblast, Proliferation, Interleukin-6

中图分类号:

Q95-33

陈芳, 朱以良, 杨李, 唐瑛. 益骨汤含药血清对新生大鼠成骨细胞增殖及分泌IL-6的影响[J]. 实验动物与比较医学, 2012, 32(2): 146-148.

CHEN Fang, ZHU Yi-liang, YANG Li, TANG Ying. Effect of YIGUTANG Containing Serum on Osteoblast Proliferation and IL-6 Secretion[J]. Laboratory Animal and Comparative Medicine, 2012, 32(2): 146-148.

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益骨汤含药血清对新生大鼠成骨细胞增殖及分泌IL-6的影响

Effect of YIGUTANG Containing Serum on Osteoblast Proliferation and IL-6 Secretion

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