关键词: 输卵管上皮细胞, 共培养, 小鼠胚胎, 体外培养

Abstract: To investigate the effect of oviductal cells system on the in-vitro development of mcase 2PN fertilized eggs , the co-culture system of goat oviductal epithelial cells were established. Mouse 2PN fertilited eggs were co-cultured with goat oviductal ep-ithelial cells in M16 co-medium (test group) or in M16 medium alone (control group). The results showed that more than 86% of embryos co-cultured with the oviductal cells cleaved beyond the "in vitro" block whereas only 50% of embryos in M16 medium cleaved to the same degree (P<0. 01) . The findings suggested that the goat oviductal epithelial cells system had the beneficial effect on mouse 2PN fertilized eggs passing the "2-cell block" in-vitro.

Key words: Oviductal epithelial cells, Co-culture, Mouse embryos, In-vitro development