不同浓度弗氏佐剂对B6AF1小鼠免疫性卵巢早衰模型建立的影响

doi:10.3969/j.issn.1674-5817.2019.04.007

实验动物与比较医学 ›› 2019, Vol. 39 ›› Issue (4): 294-297.DOI: 10.3969/j.issn.1674-5817.2019.04.007

不同浓度弗氏佐剂对B6AF1小鼠免疫性卵巢早衰模型建立的影响

陈玥, 王佩娟, 陈思, 卢燕

南京中医药大学附属中西医结合医院妇产科, 南京 210028

收稿日期:2018-12-10 出版日期:2019-08-25 发布日期:2021-01-31
作者简介:陈玥(1990-),女,硕士研究生,住院医师。研究方向:中医妇科。E-mail:cy94264@126.com
基金资助:
江苏省自然科学基金面上项目(编号:BK20171511),第六批全国老中医药专家学术经验继承工作(编号:17QGSC001)

Effect of Different Freund's Adjuvant Dose on Establishment of Immune Premature Ovarian Failure Model in B6AF1 Mice

CHEN Yue, WANG Pei-juan, CHEN Si, LU Yan

Department of Obstetrics and Gynecology, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine Nanjing 210028, China

Received:2018-12-10 Online:2019-08-25 Published:2021-01-31

摘要/Abstract

摘要： 目的观察不同浓度弗氏佐剂对透明带3多肽(pZP3)诱导的B6AF1小鼠免疫性卵巢早衰(POF)模型的影响。方法取6~8周龄雌性B6AF1小鼠80只,随机分为空白组、不完全弗氏佐剂组、完全弗氏佐剂组[含结核分枝杆菌(TB)1 mg/mL)和不完全弗氏佐剂+TB(3.3 mg/mL)组, 应用HE染色法比较不同注射量的弗氏佐剂对POF 模型小鼠免疫效果的影响。结果免疫后模型组小鼠卵巢体积萎缩,闭锁卵泡增多,卵泡周围见间质淋巴细胞浸润。不完全弗氏佐剂+ TB(3.3 mg/mL)组免疫效果最好,完全弗氏佐剂组次之。结论不同浓度弗氏佐剂联合pZP3均可诱导B6AF1小鼠成功构建POF疾病模型,引起免疫性卵巢炎; 含高浓度TB造模效果较好。

关键词: 卵巢早衰(POF), 弗氏佐剂, 结核分枝杆菌(TB), 透明带3多肽(pZP3), B6AF1小鼠

Abstract: Objective To observe the effect on different doses of Fletcher’s adjuvant to B6AF1 mice with autoimmune premature ovarian failure (POF) induced by zona pellucida 3 peptide (pZP3). Methods Autoimmune POF models were established in 60 female B6AF1 mice (6-8 weeks old) by immunization with pZP3 and another 20 mice served as control group. The POF models were randomly divided into incomplete Freund’s adjuvant group, complete Freund’s adjuvant group (1 mg/mL) and incomplete Freund’s adjuvant + tuberculosis (TB) group (3.3 mg/mL). The HE staining ovary histomorphology results were observed by the microscope. Results Compared with those in control group, the mice in the model group showed a smaller volume of the ovary, and larger amount of atretic follicle of ovary. Immunohistochemical studies showed that the model mice had redundant lymphocytes infiltrating around follicle compared with control mice. The immunological effect of incomplete Freund’s adjuvant +TB (3.3 mg/mL) group was the best, followed by complete Freund’s adjuvant group. Conclusion Different concentrations of Freund’s adjuvant combined with zona pellucida 3 polypeptide (pZP3) can induce autoimmune premature ovarian failure (POF) in B6AF1 mice. High concentration of Mycobacterium tuberculosis has the best effect.

Key words: Premature ovarian failure (POF), Freund's adjuvant;, Mycobacterium tuberculosis (TB)

中图分类号:

Q95-33

陈玥, 王佩娟, 陈思, 卢燕. 不同浓度弗氏佐剂对B6AF1小鼠免疫性卵巢早衰模型建立的影响[J]. 实验动物与比较医学, 2019, 39(4): 294-297.

CHEN Yue, WANG Pei-juan, CHEN Si, LU Yan. Effect of Different Freund's Adjuvant Dose on Establishment of Immune Premature Ovarian Failure Model in B6AF1 Mice[J]. Laboratory Animal and Comparative Medicine, 2019, 39(4): 294-297.

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不同浓度弗氏佐剂对B6AF1小鼠免疫性卵巢早衰模型建立的影响

Effect of Different Freund's Adjuvant Dose on Establishment of Immune Premature Ovarian Failure Model in B6AF1 Mice

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