恶性疟原虫动物模型及基因编辑研究进展

doi:10.3969/j.issn.1674-5817.2019.01.013

实验动物与比较医学 ›› 2019, Vol. 39 ›› Issue (1): 65-71.DOI: 10.3969/j.issn.1674-5817.2019.01.013

恶性疟原虫动物模型及基因编辑研究进展

匡德宣, 王文广, 孙晓梅, 代解杰

中国医学科学院/北京协和医学院医学生物学研究所树鼩种质资源中心,云南省重大传染病疫苗研发重点实验室, 中国医学科学院医学生物学研究所实验树鼩标准化与应用研究省创新团队, 昆明650118

收稿日期:2018-08-01 出版日期:2019-02-25 发布日期:2021-01-29
作者简介:匡德宣(1974-),男,硕士,主任技师,研究方向:实验动物及疾病模型研究。E-mail:kdx@imbcams.com.cn
基金资助:
云南省技术创新人才培养项目(No. 2016HB006); 云南省科技人才和平台计划项目(No. 2017HC019); 重点实验室专项(No. KF2015-01); 云南省重大科技专项(No. 2017ZF007)

Research Progress of Animal Model Establishment and Gene Editing on Plasmodium falciparum

KUANG De-xuan, WANG Wen-guang, SUN Xiao-mei, DAI Jie-jie

Center of Tree Shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Yunnan Innovation Team of Standardization and Application Research in Tree Shrew, Kunming 650118, China

Received:2018-08-01 Online:2019-02-25 Published:2021-01-29

摘要/Abstract

摘要： 恶性疟原虫是人体疟疾的病原体,恶性疟原虫动物模型是研究人疟原虫感染、慢性致病机制、药物评价和人疟原虫疫苗研制的重要实验材料。迄今为至,恶性疟原虫动物模型研究主要涉及非人灵长类、免疫缺陷小鼠、转基因技术以及CRISPR/Cas9系统等领域。本文对恶性疟原虫体内外感染动物模型及基因编辑研究做总结分析,为今后恶性疟原虫动物模型建立及基因编辑研究提供参考。

关键词: 恶性疟原虫, 体外感染, 体内感染, 基因编辑

Abstract: Plasmodium falciparum is the pathogen of human malaria. The animal model of plasmodium falciparum is an important experimental tool to study the infection process of plasmodium falciparum, the mechanism of chronic pathogenesis, the evaluation of drugs and the vaccine on plasmodium falciparum. Up to now, the animal model research of plasmodium falciparum mainly involves non-human primates, immuno-deficient mice, transgenic technology and CRISPRR/Cas9 system. In this paper, the animal model of plasmodium falciparum in vitro/vivo infection and gene editing were summarized and analyzed to provide reference for the establishment of animal model and gene editing of plasmodium falciparum in the future.

Key words: Plasmodium falciparum, in vitro infection, in vivo infection, Gene editing

中图分类号:

Q95-33

匡德宣, 王文广, 孙晓梅, 代解杰. 恶性疟原虫动物模型及基因编辑研究进展[J]. 实验动物与比较医学, 2019, 39(1): 65-71.

KUANG De-xuan, WANG Wen-guang, SUN Xiao-mei, DAI Jie-jie. Research Progress of Animal Model Establishment and Gene Editing on Plasmodium falciparum[J]. Laboratory Animal and Comparative Medicine, 2019, 39(1): 65-71.

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恶性疟原虫动物模型及基因编辑研究进展

Research Progress of Animal Model Establishment and Gene Editing on Plasmodium falciparum

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