大、小鼠绿脓杆菌脉冲场凝胶电泳分型研究

doi:10.3969/j.issn.1674-5817.2016.06.003

实验动物与比较医学 ›› 2016, Vol. 36 ›› Issue (6): 419-422.DOI: 10.3969/j.issn.1674-5817.2016.06.003

大、小鼠绿脓杆菌脉冲场凝胶电泳分型研究

冯洁^1,2, 谢建云², 魏晓锋², 胡建华², 高诚²

1.扬州大学兽医学院, 扬州 225009;
2.上海实验动物研究中心, 上海市实验动物质量监督检验站, 上海 201203

收稿日期:2016-07-22 出版日期:2016-12-25 发布日期:2016-12-25
作者简介:冯洁(1981-), 女, 副研究员。E-mail: moyifj@163.com
基金资助:
上海市科技创新行动计划(14140900600、12140900500), 上海市科委研发平台专项(15DZ2292400)

Molecular Subtyping Study on Pseudomonas aeruginosa in Rats and Mice with Pulsed Field Gel Electrophoresis

FENG Jie^1,2, XIE Jian-yun², WEI Xiao-feng², HU Jian-hua², GAO Cheng²

1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;
2. Shanghai Laboratory Animal Research Center, Shanghai Quality Monitoring Center of Laboratory Animals, Shanghai 201203, China

Received:2016-07-22 Online:2016-12-25 Published:2016-12-25

摘要/Abstract

摘要： 目的对近年来上海及江苏地区部分单位大、小鼠绿脓杆菌进行分离鉴定,采用脉冲场凝胶电泳(PFGE)分子分型技术作基因型分析,以了解绿脓杆菌流行菌株与宿主之间的相关性。方法无菌采集上海及江苏地区部分单位实验大、小鼠回盲部内容物进行绿脓杆菌分离培养,采用形态学观察、生化试验对分离菌进行鉴定。提取绿脓杆菌分离株基因组DNA,经Xba I酶切后进行脉冲场凝胶电泳。结果共分离到67株绿脓杆菌,PFGE图谱可分为39种不同带型,14个基因簇(A-N),优势簇分别为I、H、B和G,分别占17.9%(12/67)、14.9%(10/67)、13.4%(9/67)和 11.9%(8/67)。同一来源地的分离株大多呈现不同的基因簇。结论 67株细菌具有很大的基因多态性,呈散在分布,未见明显的流行特征。

关键词: 绿脓杆菌, 脉冲场凝胶电泳(PFGE), 分子分型

Abstract: Objective To investigate the correlation between Pseudomonas aeruginosa epidemic strains and host in recent years, Pseudomonas aeruginosa in rat and mice from several faciliies of Shanghai and Jiangsu province were isolated and detected by pulsed field gel electrophoresis (PFGE). Methods The bacteria were isolated from the contents of ileocecal junction under aseptic conditions. The isolated strains were identified by morphological observation and physicochemical test. Extracted genomic DNA of Pseudomonas aeruginosa isolates were detected by PFGE after digested by Xba I restriction enzyme. Results A total of 67 strains were isolated from rats and mice. The genotype of 67 strains was divided into 39 patterns and 14 clusters (A-N). The dominant cluster is I, H, B and G, the rate of these cluster were 17.9%(12/67), 14.9%(10/67), 13.4%(9/67) and 11.9%(8/67) respectively. The isolates from the same source mostly present different gene cluster. Conclusion A great deal of gene polymorphism could be found in all the 67 strains which are diffusively distributed. Obvious prevalent feature could not be found.

Key words: Pseudomonas aeruginosa, Pulsed field gel electrophoresis (PFGE), Molecular subtyping

中图分类号:

Q95-33

冯洁, 谢建云, 魏晓锋, 胡建华, 高诚. 大、小鼠绿脓杆菌脉冲场凝胶电泳分型研究[J]. 实验动物与比较医学, 2016, 36(6): 419-422.

FENG Jie, XIE Jian-yun, WEI Xiao-feng, HU Jian-hua, GAO Cheng. Molecular Subtyping Study on Pseudomonas aeruginosa in Rats and Mice with Pulsed Field Gel Electrophoresis[J]. Laboratory Animal and Comparative Medicine, 2016, 36(6): 419-422.

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大、小鼠绿脓杆菌脉冲场凝胶电泳分型研究

Molecular Subtyping Study on Pseudomonas aeruginosa in Rats and Mice with Pulsed Field Gel Electrophoresis

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