慢性膀胱炎相关膀胱过度活动症大鼠模型的建立

doi:10.3969/j.issn.1674-5817.2016.05.003

实验动物与比较医学 ›› 2016, Vol. 36 ›› Issue (5): 340-344.DOI: 10.3969/j.issn.1674-5817.2016.05.003

慢性膀胱炎相关膀胱过度活动症大鼠模型的建立

高峰¹, 张士更², 张楠², 张布衣², 戴玉樑¹, 李绍江¹

1.浙江中医药大学第二临床医学院, 杭州 310053;
2.浙江大学医学院附属第二医院泌尿外科, 杭州 310009

收稿日期:2016-05-26 出版日期:2016-10-25 发布日期:2016-10-25
作者简介:高峰(1990-), 男, 硕士研究生。E-mail: 934563340@qq.com
基金资助:
浙江省中医药科技计划项目(2012ZA058)

Establishment of Over Active Bladder Model Associated with Chronic Cystitis in Rat

GAO Feng, ZHANG Shi-geng, ZHANG Nan, ZHANG Bu-yi, DAI Yu-liang, LI Shao-jiang

1. The 2nd Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China;
2. Department of Urology, The Second Affiliated Hospital Zhejiang University School of Medicine, Hangzhou 310009, China

Received:2016-05-26 Online:2016-10-25 Published:2016-10-25

摘要/Abstract

摘要： 目的建立一种发病机制、尿流动力学表现与临床相近的慢性膀胱炎相关膀胱过度活动症(OAB)大鼠模型。方法将40只雌性SD大鼠随机分为模型组和生理盐水对照组; 模型组以大肠埃希氏菌膀胱灌注,3 d后搜集尿液常规显微镜检查,证实膀胱炎并以庆大霉素灌胃每日4次,连续7 d,第10日复查尿常规,每隔10 d为1个周期,经4个周期后行尿流动力学检查及膀胱HE染色病理学检查。生理盐水对照组用等量生理盐水代替大肠埃希氏菌溶液,其余处理同模型组。2组同条件饲养。结果模型组膀胱标本可见少量上皮组织退变、凋亡、脱落,黏膜固有层见散在的淋巴细胞,行尿流动力学检查确认13只建模成功。结论通过大肠埃希氏菌ATCC35218反复膀胱灌注诱导SD大鼠慢性膀胱炎,最终可建立慢性膀胱炎相关OAB大鼠模型,为临床上常见的膀胱炎后OAB的发病机制及治疗、研究提供思路。

关键词: 慢性膀胱炎, 膀胱过度活动症(OAB), 大肠埃希氏菌, 大鼠模型

Abstract: Objective To build the over active bladder (OAB) related to chronic cystitis model which is similar to clinical process including pathogenesis and urodynamic performance. Methods Forty female SD rats were randomly divided into model group and saline control group. Model group: the bladders were instilled with Escherichia coli ATCC35218 (1×10⁸~1.2×10⁸CFU/mL) 100 μL, and a urine test were done after 3 days to confirm cystitis. The rats were given by gavage with Gentamicin (0.5 mL, 2 million units) 4 times a day × 7 days and urine were retested 10 days a cycle. The rats were done urodynamics and HE staining for bladder tissue after 4 cycles. Saline control group: were given by gavage with the same amount of saline solution instead of Escherichia coli, the remaining process was the same as the model group. The animals were raised under the same conditions. Results A small amount of epithelial was found degeneration, apoptosis, loss, and scattered lymphocytes in the lamina propria in model group. Thirteen rats were successfully modeled. Conclusion The OAB animal model can be established after cystitis by repeated E.coli infusion into bladder of female SD rats’. It offers ideas for pathogenesis, treatment and research about OAB associated with chronic cystitis.

Key words: Chronic cystitis, Overactive bladder (OAB), Escherichia coli, Rat model

中图分类号:

Q95-33

高峰, 张士更, 张楠, 张布衣, 戴玉樑, 李绍江. 慢性膀胱炎相关膀胱过度活动症大鼠模型的建立[J]. 实验动物与比较医学, 2016, 36(5): 340-344.

GAO Feng, ZHANG Shi-geng, ZHANG Nan, ZHANG Bu-yi, DAI Yu-liang, LI Shao-jiang. Establishment of Over Active Bladder Model Associated with Chronic Cystitis in Rat[J]. Laboratory Animal and Comparative Medicine, 2016, 36(5): 340-344.

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慢性膀胱炎相关膀胱过度活动症大鼠模型的建立

Establishment of Over Active Bladder Model Associated with Chronic Cystitis in Rat

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