大鼠颈总动脉球囊拉伤所致血管狭窄模型的建立

doi:10.3969/j.issn.1674-5817.2015.02.001

实验动物与比较医学 ›› 2015, Vol. 35 ›› Issue (2): 87-91.DOI: 10.3969/j.issn.1674-5817.2015.02.001

• 论著 • 下一篇

大鼠颈总动脉球囊拉伤所致血管狭窄模型的建立

杨波¹, 吴冰¹, 唐俊明², 杨建业², 曹腾¹, 王家宁²

1.武汉大学人民医院心内科, 武汉 430060;
2.湖北医药学院附属人民医院临床医学研究所, 十堰 442000

收稿日期:2014-12-05 出版日期:2015-04-25 发布日期:2015-04-25
作者简介:杨波(1963-), 博士生导师, 教授。E-mail: yybb112@whu.edu.cn;吴冰(1981-)硕士研究生。E-mail: 81532468@qq.com
基金资助:
国家自然科学基金资助项目(No. 81270221)

Establishment of Vascular Restenosis Model Induced by Carotid Artery Balloon Injury in Rats

YANG Bo¹, WU Bing¹, TANG Jun-ming², YANG Jian-ye², CAO Teng¹, WANG Jia-ning²

1. Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China;
2. Institute of Clinical Medicine Renmin Hospital, Hubei University of Medicine, Shiyan 442000, China

Received:2014-12-05 Online:2015-04-25 Published:2015-04-25

摘要/Abstract

摘要： 目的建立一种简便实用的大鼠颈总动脉球囊拉伤的方法, 为研究物理损伤基础上的血管形态、细胞、生物化学成份等的变化提供稳定可靠的动物模型。方法 30只体质量450~500 g雄性SD大鼠, 异氟烷麻醉后, 分离颈总动脉、颈外动脉和颈内动脉, 并在颈外动脉上做切口,然后用2F球囊导管沿切口插入颈总动脉, 插入约4 cm后, 注入0.02 mL生理盐水使球囊膨胀,轻轻旋转着拉出导管, 反复3次, 致使颈总动脉内膜完全剥脱, 在其损伤后1 d、3 d、7 d、14 d、28 d分别取材, 并进行病理组织学观察。结果损伤后随着时间的推移新生内膜明显增生, 14 d增生达到高峰。结论改进的大鼠颈总动脉球囊拉伤模型方法简便, 手术成功率高, 并且血栓形成率低。

关键词: 颈总动脉, 血管再狭窄, 球囊拉伤, 2F球囊导管, 新生内膜增生

Abstract: Objective To establish a simple and useful method of rat carotid artery balloon injury, providing stable and reliable animal model for studying the changes of vessels based on physical damage. Methods Rat common carotid artery, external carotid artery and internal carotid artery were isolated after anesthesia with isoflurane, the artery incision was performed in the external carotid artery, and then the 2F balloon catheter was inserted into common carotid artery in the incision, and pushed about 4 cm. Meanwhile, 0.02 mL normal saline was slightly injected to make the balloon inflate, and then the catheter was gently rotated for all the way when removing. Repeat this operation by three times to completely denude the common carotid artery intima. The changes of histomorphology in carotid artery were observed 1 day, 3 days, 7 days, 14 days, and 28 days after injury. Results Obvious neointima hyperplasia appeared over time after damage, which reached the peak at 14 days. Conclusion The improved rat carotid artery balloon injury model is established with the characteristics of simple operation, high success rate, and low rate of thrombosis.

Key words: Common carotid artery, Vascular restenosis, Balloon withdraw injury, 2F Fogarty arterial embolectomy balloon catheter, Neointimal hyperplasia

中图分类号:

Q95-33

杨波, 吴冰, 唐俊明, 杨建业, 曹腾, 王家宁. 大鼠颈总动脉球囊拉伤所致血管狭窄模型的建立[J]. 实验动物与比较医学, 2015, 35(2): 87-91.

YANG Bo, WU Bing, TANG Jun-ming, YANG Jian-ye, CAO Teng, WANG Jia-ning. Establishment of Vascular Restenosis Model Induced by Carotid Artery Balloon Injury in Rats[J]. Laboratory Animal and Comparative Medicine, 2015, 35(2): 87-91.

参考文献

[1] Ananthanarayanan B, Fosbrink M, Rahdar M, et al.Live-cell molecular analysis of Akt activation reveals roles for activation loop phosphorylation[J]. J Biol Chem, 2007, 282(50):36634-36641.
[2] Lemos PA, van Mieghem CA, Arampatzis CA, et al. Post-sirolimus-eluting stent restenosis treated with repeat percutaneous intervention: late angiographic and clinical outcomes[J]. Circulation, 2004, 109(21):2500-2502.
[3] Costa MA, Simon DI.Molecular basis of restenosis and drug-eluting stents[J]. Circulation, 2005, 111(17):2257-2273.
[4] Clowes AW, Reidy MA, Clowes MM.Kinetics of cellular proliferation after arterial injury. I. Smooth muscle growth in the absence of endothelium[J]. Lab Invest, 1983, 49(3):327-333.
[5] Harada M, Toki Y, Numaguchi Y, et al.Prostacyclin synthase gene transfer inhibits neointimal formation in rat balloon-injured arteries without bleeding complications[J]. Cardiovasc Res, 1999, 43(2):481-491.
[6] Ohtani K, Egashira K, Hiasa K, et al.Blockade of vascular endothelial growth factor suppresses experimental restenosis after intraluminal injury by inhibiting recruitment of monocyte lineage cells[J]. Circulation, 2004, 110(16):2444-2452.
[7] Kong D, Melo LG, Gnecchi M, et al.Cytokine-induced mobilization of circulating endothelial progenitor cells enhances repair of injured arteries[J]. Circulation, 2004, 110(14):2039-2046.
[8] Clowes AW, Reidy MA, Clowes MM.Mechanisms of stenosis after arterial injury[J]. Lab Invest, 1983, 49(2):208-215.
[9] Clowes AW, Clowes MM.Kinetics of cellular proliferation after arterial injury. II. Inhibition of smooth muscle growth by heparin[J]. Lab Invest, 1985, 52(6):611~616.
[10] Clowes AW, Clowes MM, Reidy MA.Kinetics of cellular proliferation after arterial injury. III. Endothelial and smooth muscle growth in chronically denuded vessels[J]. Lab Invest,1986, 54(3):295-303.
[11] Tulis DA.Rat carotid artery balloon injury model[J]. Methods Mol Med, 2007, 139:1-30.
[12] Wang JN, Shi N, Xie WB, et al.Response gene to complement 32 promotes vascular lesion formation through stimulation of smooth muscle cell proliferation and migration[J]. Arterioscler Thromb Vasc Biol, 2011, 31(8):e19-e26.
[13] Touchard AG, Schwartz RS.Preclinical restenosis models: challenges and successes[J]. Toxicol Pathol, 2006, 34(1):11-18.
[14] Min SK, Min SI, Jeong EM, et al.Intimal hyperplasia in loop-injured carotid arteries is attenuated in transglutaminase 2-null mice[J]. J Korean Med Sci, 2014, 29(3):363-369.
[15] Sata M, Maejima Y, Adachi F, et al.A mouse model of vascular injury that induces rapid onset of medial cell apoptosis followed by reproducible neointimal hyperplasia[J]. J Mol Cell Cardiol, 2000, 32(11):2097-2104.

大鼠颈总动脉球囊拉伤所致血管狭窄模型的建立

Establishment of Vascular Restenosis Model Induced by Carotid Artery Balloon Injury in Rats

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