裸鼹鼠肺脏显微结构与超微结构观察

doi:10.3969/j.issn.1674-5817.2013.05.012

实验动物与比较医学 ›› 2013, Vol. 33 ›› Issue (5): 378-382.DOI: 10.3969/j.issn.1674-5817.2013.05.012

裸鼹鼠肺脏显微结构与超微结构观察

袁子彦¹, 赵懿宁², 张璐¹, 赵善民¹, 林丽芳¹, 汤球¹, 孙伟¹, 余琛琳¹, 蔡丽萍¹, 徐晨¹, 崔淑芳¹

1.第二军医大学实验动物中心, 上海200433;
2.上海市杨浦区精神卫生中心, 上海200090

收稿日期:2013-07-15 出版日期:2013-10-25 发布日期:2013-10-25
作者简介:袁子彦(1984-), 女, 硕士, 助教,E-mail: yuanziyanyy@163.com
基金资助:
上海市科技发展基金项目(No. 11140901700)与 (No.12140900200)联合资助

Observation on Micro- and Ultra-structures of the Lung in Naked Mole Rar (Heterocephalus glaber)

YUAN Zi-yan¹, ZHAO Yi-ning², ZHANG Lu¹, ZHAO Shan-min¹, LIN Li-fang¹, TANG Qiu¹, Sun Wei¹, YU Chen-ling¹, CAI Li-ping¹, XU Chen¹, CUI Shu-fang¹

1. Laboratory Animal Center, Second Military Medical University, Shanghai 200433, China;
2. Yangpu district mental health center of Shanghai, Shanghai 200090, China

Received:2013-07-15 Online:2013-10-25 Published:2013-10-25

摘要/Abstract

摘要： 目的观察和分析裸鼹鼠肺脏的形态结构与组成。方法采集裸鼹鼠肺脏经石蜡切片苏木精-伊红染色,然后光学显微镜下观察裸鼹鼠肺脏的显微结构,以及经冰冻超薄切片和染色后,通过透射电镜观察和分析裸鼹鼠心脏的超微结构。结果光学显微镜观察发现裸鼹鼠肺脏有类似支气管扩张和肺不张等病理变化,而且肺血管分布情况和支气管上皮细胞的形态和分布与小鼠有很大不同;透射电镜观察裸鼹鼠肺脏并没有支扩和肺不张的病理变化,肺泡上皮细胞有微绒毛,裸鼹鼠肺脏气血屏障有增厚现象。结论裸鼹鼠肺脏呈幼态性发育,而且肺泡可根据机体代谢率和需氧量的不同而进行开启和关闭。

关键词: 裸鼹鼠, 肺脏, 显微结构, 超微结构

Abstract: Objective To observe and analyze the the morphological structure and composition of lung in the naked mole rat (Heterocephalus glaber). Method The lungs were isolated from anaesthetic animals and then made to paraffin sections, which were stained by hematoxylin-eosin (HE) dye. The microstructure of lung was observed under optical microscope. Meanwhile, the fresh lungs were processed to ultrathin sections and stained. At last, the ultramicroscopic structure of lungs was observed by transmission electron microscope. Result The lungs of NMR looks like bronchiectasis and pulmonary atelectasis observation through optical microscope. Besides, the distribution of vasculars and bronchial epithelial cells are quite different from mice. And, the surface of alveolar epithelial cells in NMR are rich in microvilli. Moreover, the blood-gas barrier of NMR is thiker then mice’s. Conclusion The lungs of NMR show infantile development. More importantly, NMR can open or close alveolar according to the body’s metabolic rate and oxygen demand.

Key words: Naked mole rat, Lung, Microstructure, Ultrastructure

中图分类号:

Q95-33

袁子彦, 赵懿宁, 张璐, 赵善民, 林丽芳, 汤球, 孙伟, 余琛琳, 蔡丽萍, 徐晨, 崔淑芳. 裸鼹鼠肺脏显微结构与超微结构观察[J]. 实验动物与比较医学, 2013, 33(5): 378-382.

YUAN Zi-yan, ZHAO Yi-ning, ZHANG Lu, ZHAO Shan-min, LIN Li-fang, TANG Qiu, Sun Wei, YU Chen-ling, CAI Li-ping, XU Chen, CUI Shu-fang. Observation on Micro- and Ultra-structures of the Lung in Naked Mole Rar (Heterocephalus glaber)[J]. Laboratory Animal and Comparative Medicine, 2013, 33(5): 378-382.

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裸鼹鼠肺脏显微结构与超微结构观察

Observation on Micro- and Ultra-structures of the Lung in Naked Mole Rar (Heterocephalus glaber)

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