无菌动物在人类健康和疾病研究中应用

doi:10.3969/j.issn.1674-5817.2012.03.018

实验动物与比较医学 ›› 2012, Vol. 32 ›› Issue (3): 250-253.DOI: 10.3969/j.issn.1674-5817.2012.03.018

无菌动物在人类健康和疾病研究中应用

曾本华, 魏泓

第三军医大学基础部实验动物教研室, 重庆 400038

收稿日期:2011-11-16 出版日期:2012-06-25 发布日期:2012-06-25
作者简介:曾本华(1980-), 男, 博士研究生, 从事悉生动物模型研究; E-mail: zengbh1980@yahoo.com.cn
基金资助:
国家重点基础研究发展计划(973计划)(项目编号: 2007CB513007).

Application of Germ Free Animals in Study of Human Health and Disease

ZENG Ben-hua, WEI Hong

Department of Laboratory Animal Science, Collegy of Preclinical Medicine,Third Military Medical University, Chongqing 400038, China

Received:2011-11-16 Online:2012-06-25 Published:2012-06-25

摘要/Abstract

摘要： 随着肠道菌群与人类疾病及健康的关系研究的深入, 已证实肠道菌群不仅构成黏膜屏障、参与食物消化、刺激免疫及胃肠功能发育,而且作为内化的环境因素参与肥胖、糖尿病、IBD、肿瘤等多种疾病的发生,肠道菌群与人类疾病及健康关系研究的深入对动物模型提出了新要求。无菌动物作为微生物背景最清晰的动物,可有效地对菌群、基因及其它环境因素进行控制,是研究肠道菌群对人类疾病及健康的影响的最有效的动物模型。利用无菌动物构建的悉生动物模型及菌群人源化动物(HHA动物)模型已在肠道菌群对宿主生理功能的影响、代谢性疾病发生、药物代谢等研究领域广泛应用。

关键词: 无菌动物, 肠道菌群, 悉生动物, HFA动物

Abstract: Gut microbiota has been reported to involve in host physiology functions, certain pathological disorders, and drug metabolism. The gut microbiota colonization is also involved in several important development of physiological function, especially development of immune system and digestive system. As the cleanest animal associated no microorganism, germ free animal is a precondition to study the relationship between microbiota and human health state. Germ free animal, gnotobiotic animals and human flora associated animals have been widely used in studying the effect of microbiota on host development, etiological factor of metabolic disease and drug metabolism.

Key words: Germ free animal, Gut microbiota, Gnotobiotic animals, Human flora associated animal

中图分类号:

Q95-33

曾本华, 魏泓. 无菌动物在人类健康和疾病研究中应用[J]. 实验动物与比较医学, 2012, 32(3): 250-253.

ZENG Ben-hua, WEI Hong. Application of Germ Free Animals in Study of Human Health and Disease[J]. Laboratory Animal and Comparative Medicine, 2012, 32(3): 250-253.

参考文献

[1] Travnicek J, Mandel L.Gnotobiotic techniques[J]. Folia Microbiol (Praha), 1979, 24(1):6-10.
[2] Inzunza J, Midtvedt T, Fartoo M, et al.Germfree status of mice obtained by embryo transfer in an isolator environment[J]. Lab Anim, 2005, 39(4):421-427.
[3] Faith JJ, Rey FE, O'Donnell D, et al. Creating and characterizing communities of human gut microbes in gnotobiotic mice[J]. ISME J, 2010, 4(9):1094-1098.
[4] Falk PG, Hooper LV, Midtvedt T, et al.Creating and maintaining the gastrointestinal ecosystem: what we know and need to know from gnotobiology[J]. Microbiol Mol Biol Rev, 1998, 62(4):1157-1170.
[5] Lederberg J.Infectious history[J]. Science (80-), 2000,288(5456):287-293.
[6] Li M, Wang B, Zhang M, Rantalainen M, et al.Symbiotic gut microbes modulate human metabolic phenotypes[J]. Proc Natl Acad Sci U S A, 2008, 105(6):2117-2122.
[7] Guarner F, Malagelada JR.Gut flora in health and disease[J]. Lancet, 2003,361(9371):512-519.
[8] Backhed F, Manchester JK, Semenkovich CF, et al.Mechanisms underlying the resistance to diet-induced obesity in germ-free mice[J]. Proc Natl Acad Sci U S A, 2007, 104(3):979-984.
[9] Sudo N, Chida Y, Aiba Y, et al.Postnatal microbial colonization programs the hypothalamic-pituitary-adrenal system for stress response in mice[J]. J Physiol, 2004, 558(1):263-275.
[10] Preidis GA, Versalovic J.Targeting the human microbiome with antibiotics, probiotics, and prebiotics: gastroenterology enters the metagenomics era[J]. Gastroenterology, 2009,136(6):2015-2031.
[11] Inzunza J, Midtvedt T, Fartoo M, et al.Germfree status of mice obtained by embryo transfer in an isolator environment[J]. Lab Anim, 2005,39(4):421-427.
[12] Hirayama K, Itoh K.Human flora-associated (HFA) animals as a model for studying the role of intestinal flora in human health and disease[J]. Curr Issues Intest Microbiol, 2005, 6(2):69-75.
[13] Silley P.Human flora-associated rodents ?does the data support the assumptions?[J]. Microb Biotechnol, 2009, 2(1):6-14.
[14] Che C, Pang X, Hua X, et al.Effects of human fecal flora on intestinal morphology and mucosal immunity in human flora-associated piglet[J]. Scand J Immunol, 2009, 69(3):223-233.
[15] Pang X, Hua X, Yang Q, et al.Inter-species transplantation of gut microbiota from human to pigs[J]. ISME J, 2007, 1(2):156-162.
[16] Lofgren JL, Whary MT, Ge Z, et al.Lack of commensal flora in Helicobacter pylori-infected INS-GAS mice reduces gastritis and delays intraepithelial neoplasia[J]. Gastroenterology, 2011, 140(1):210-220.
[17] Denou E, Rezzonico E, Panoff JM, et al.A Mesocosm of Lactobacillus johnsonii, Bifidobacterium longum, and Escherichia coli in the mouse gut[J]. DNA Cell Biol, 2009, 28(8):413-422.
[18] Duncan BB, Schmidt MI, Pankow JS, et al.Low-grade systemic inflammation and the development of type 2 diabetes: the atherosclerosis risk in communities study[J]. Diabetes, 2003, 52(7):1799-1805.
[19] Visser M, Bouter LM, McQuillan GM, et al. Elevated C-reactive protein levels in overweight and obese adults[J]. JAMA, 1999, 282(22):2131-2135.
[20] Hotamisligil GS, Shargill NS, Spiegelman BM.Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance[J]. Science (80-), 1993, 259(5091):87-91.
[21] Cani PD, Amar J, Iglesias MA, et al.Metabolic endotoxemia initiates obesity and insulin resistance[J]. Diabetes, 2007, 56(7):1761-1772.
[22] Cani PD, Neyrinck AM, Fava F, et al.Selective increases of bifidobacteria in gut microflora improve high-fat-diet-induced diabetes in mice through a mechanism associated with endotoxaemia[J]. Diabetologia, 2007, 50(11):2374-2383.
[23] Toda T, Saito N, Ikarashi N, et al.Intestinal flora induces the expression of Cyp3a in the mouse liver[J]. Xenobiotica, 2009,39(4):323-334.
[24] Toda T, Ohi K, Kudo T, et al.Antibiotics suppress Cyp3a in the mouse liver by reducing lithocholic acid-producing intestinal flora[J]. Yakugaku Zasshi, 2009, 129(5):601-608.

无菌动物在人类健康和疾病研究中应用

Application of Germ Free Animals in Study of Human Health and Disease

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 4

编辑推荐

Metrics

本文评价

[1]	许琴, 倪艳, 是文辉, 李建瑛, 刘江伟, 赵红琼, 徐新明. SPF级雄性SD大鼠回肠与结肠菌群结构比较分析[J]. 实验动物与比较医学, 2023, 43(1): 53-60.
[2]	王晓钰, 蒋升瑶, 蔺智兵, 崔立. 基于糖尿病-肿瘤小鼠模型研究糖尿病对结直肠癌/乳腺癌进展及肠道菌群的影响[J]. 实验动物与比较医学, 2021, 41(6): 469-479.
[3]	许琴, 董翔, 李建瑛, 张东辉, 李佳佳, 杨亚萍, 杨秋月, 是文辉, 马娜, 宋来阳, 刘江伟. 姜黄素预处理对沙漠干热中暑大鼠回肠、结肠内容物菌群表达量的影响[J]. 实验动物与比较医学, 2018, 38(6): 440-445.
[4]	戴方伟1, 周莎桑2, 柯贤福2, 徐智3, 萨晓婴2. 国内无菌动物无菌检查的现状与分析[J]. , 2006, 26(4): 261-264.