采用小动物活体荧光成像对胃癌皮下和原位移植肿瘤生长的动态观察

doi:10.3969/j.issn.1674-5817.2011.05.015

实验动物与比较医学 ›› 2011, Vol. 31 ›› Issue (5): 371-375.DOI: 10.3969/j.issn.1674-5817.2011.05.015

采用小动物活体荧光成像对胃癌皮下和原位移植肿瘤生长的动态观察

彭秀华¹, 沈艳¹, 徐春华¹, 杨玉琴¹, 周文江^1,2

1.上海市公共卫生临床中心, 上海 201508;
2.复旦大学药学院, 上海201203

收稿日期:2010-09-27 修回日期:2011-06-25 出版日期:2011-10-15 发布日期:2011-10-25
作者简介:彭秀华(1973-),女,兽医师,主要从事实验动物学研究,E-mail:pengxh1973@sina.com

Dynamic Observation On Growth of Subcutaneous and Orthotopic Gastric Tumor by in vivo Fluorescence Imager

PENG Xiu-Hua¹, SHEN Yan¹, XU Chun-Hua¹, YANG Yu-Qin¹, ZHOU Wen-Jiang^1,2

1. Shanghai Public Health Clinical Center,Shanghai 201508,China;
2. College of Pharmacy,Fudan University,Shanghai 201203,China

Received:2010-09-27 Revised:2011-06-25 Online:2011-10-15 Published:2011-10-25

摘要/Abstract

摘要： 目的采用小动物活体荧光成像系统对裸小鼠皮下和原位移植MKN-45-Luc胃癌细胞组织瘤块后,进行肿瘤生长的动态观察。方法将转染Luc的MKN-45 胃癌细胞接种于裸小鼠皮下,成瘤后取瘤块分别接种于裸小鼠的皮下和胃部,并用活体荧光成像系统定期监测MKN-45-Luc细胞在小鼠体内成瘤的动态变化过程。结果从第1~5周,随着肿瘤移植时间的增加,皮下和原位瘤所表达荧光素酶的面积逐渐增大,荧光光子数也逐渐增加,移植瘤体积、荧光面积与荧光光子数成正相关(r=0.882, P<0.001)。5~6周时移植瘤体积、荧光面积与荧光光子数无相关性。结论采用活体荧光成像系统能非常完整地观察活体动物体内胃癌的生长及转移过程。为研究MKN-45胃癌生长的生物学特性提供依据。

关键词: 活体荧光成像, 皮下和原位移植, 胃癌, 荧光素酶

Abstract: Objective To observe the growth profile of MKN-45-Luc gastric tumor in nude mice by fluorescence imaging system of small animals in vivo , after the tumor transplantation into the nude mice through subcutaneous or orthotopic transplantation. Methods To inoculate subcutaneously the MKN-45 gastric cancer cells which were transfected Luc into nude mice, when the tumor formed, it were harvested and inoculated subcutaneously or directly transplanted into the stomach of nude mice. AT the same time, we used the fluorescence imaging system of small animals in vivo to monitor the dynamic changes of MKN-45-Luc cells in mice. Results From the first week to the fifth week, both the luciferase expressed area of the subcutaneous and orthotopic tumor and the fluorescent intensity were gradually increased with the time increase of tumor transplantation . A positive correlation existed among the tumor volume, fluorescence area and fluorescent intensity (r=0.882, P<0.001), which disappeared after 5-6 weeks following the inoculation. Conclusions The fluorescence imaging systems of small animals in vivo were untilized to fully observe the growth and metastasis profile of gastric cancer, which provided the basis for investigating the biological characteristics of the MKN-45 gastric cancer.

Key words: Fluorescence imaging, Subcutaneous and orthotopic transplantation, Gastric cancer, Luciferase

中图分类号:

Q95-33

彭秀华, 沈艳, 徐春华, 杨玉琴, 周文江. 采用小动物活体荧光成像对胃癌皮下和原位移植肿瘤生长的动态观察[J]. 实验动物与比较医学, 2011, 31(5): 371-375.

PENG Xiu-Hua, SHEN Yan, XU Chun-Hua, YANG Yu-Qin, ZHOU Wen-Jiang. Dynamic Observation On Growth of Subcutaneous and Orthotopic Gastric Tumor by in vivo Fluorescence Imager[J]. Laboratory Animal and Comparative Medicine, 2011, 31(5): 371-375.

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采用小动物活体荧光成像对胃癌皮下和原位移植肿瘤生长的动态观察

Dynamic Observation On Growth of Subcutaneous and Orthotopic Gastric Tumor by in vivo Fluorescence Imager

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