滇南小耳猪与云南野猪SLA-DQA基因的多态性比较

doi:10.3969/j.issn.1674-5817.2011.05.012

实验动物与比较医学 ›› 2011, Vol. 31 ›› Issue (5): 360-364.DOI: 10.3969/j.issn.1674-5817.2011.05.012

滇南小耳猪与云南野猪SLA-DQA基因的多态性比较

刘荣辉^1,2,3, 卢丽仪¹, 陶璇^1,2, 于辉¹, 李华¹

1.佛山科学技术学院动物科学系, 南海 528231;
2.四川农业大学动物科技学院, 雅安 625014;
3.重庆市开县畜牧兽医局, 重庆 405400

收稿日期:2011-05-30 出版日期:2011-10-15 发布日期:2011-10-25
作者简介:刘荣辉(1984-),男,硕士研究生,研究方向为动物遗传育种。E-mail:jerry13023@126.com
基金资助:
广东省高等学校高层次人才项目、广东现代农业生猪产业体系项目及佛山市科技发展专项基金(2008B01012)资助

The Comparison of Genetic Polymorphism of SLA-DQA Gene Between Diannan Small-Ear Pig and Yunnan Wild Boar

LIU Rong-hui^1,2,3, LU Li-yi¹, TAO Xuan^1,2, YU Hui¹, LI Hua¹

1. Department of Animal Science, Foshan University, Nanhai 528231, China;
2. College of Animal Science and Technology of Sichuan Agriculture University, Ya’an 625014,China;
3. The Kaixian Bureau of Husbandry and Veteriary, Chongqing 400000,China

Received:2011-05-30 Online:2011-10-15 Published:2011-10-25

摘要/Abstract

摘要： 目的分析滇南小耳猪与云南野猪(S. s. silvianus) SLA-DQA基因不同酶切位点的基因型多态性以及等位基因的多态性,检验这些酶切位点上的基因频率是否达到Hardy-Weinberg平衡。方法采用EcoRⅠ和AluⅠ两种限制性内切酶对滇南小耳猪和云南野猪SLA-DQA第1和第2内含子部分序列以及完整的外显子2进行PCR-RFLP分析。结果在EcoRⅠ与Alu I酶切位点上均得到3种基因型,在这两个酶切位点上版纳系有9种组合基因型,明光系有8种组合基因型,而野猪仅发现4种组合基因型。经χ²适合性检验表明: 滇南小耳猪SLA-DQA外显子2在EcoR I与Alu I酶切位点上均未达到Hardy-Weinberg平衡状态(P<0.05),但明光系和云南野猪在两酶切位点均达到了平衡状态(P>0.05)。结论滇南小耳猪SLA-DQA在EcoR I与Alu I酶切位点均表现为中度多态, 云南野猪在EcoR I为中度多态, 在Alu I酶切位点则为低度多态; 滇南小耳猪的Hardy-Weinberg不平衡主要来自版纳系小耳猪。

关键词: 滇南小耳猪, 云南野猪, SLA-DQA, RFLP, 遗传特性

Abstract: Objective Analyzed genotype and allelic polymorphism of SLA-DQA gene in Diannan small-ear pig and Yunnan wild boar (YWB, S. s. silvianus) to detect whether the genotype frequency of restriction enzyme sites was fit with Hardy-Weinberg equilibrium. Methods PCR-RFLP was used to analyze the partial sequences of intron 1 and intron 2 and the complete sequence of extron 2 of SLA-DQA in Diannan small-ear pig(DN) and Yunnan wild boar. Result Both EcoRⅠand AluⅠdigested sites had three kinds of genotypes, There were nine composite genotypes in Banna pig(BN) and eight in Mingguang pig(MG), but only four composite genotypes in Yunnan wild boar. The χ² test value of genotypes at both EcoRⅠand AluⅠsites in Diannan small-ear pig wasn’t fit with Hardy-Weinberg equilibrium (P<0.05), versus in Mingguang pig and Yunnan wild boar was fit with Hardy-Weinberg equilibrium, respectively (P>0.05). Conclusion Genetic polymorphism of SLA-DQA at both EcoR˘ńand AluⅠsites in Diannan small-ear pig is moderate, at EcoRⅠsite in Yunnan wild boar is moderate versus at AluⅠsite in Yunnan wild boar is low; the disequilibrium of Hardy-Weinberg in Diannan small-ear pig is majorly resulted from Banna pig.

Key words: Diannan small-ear pig, Yunnan wild boar, SLA-DQA, RFLP, Hereditary character

中图分类号:

Q95-33

刘荣辉, 卢丽仪, 陶璇, 于辉, 李华. 滇南小耳猪与云南野猪SLA-DQA基因的多态性比较[J]. 实验动物与比较医学, 2011, 31(5): 360-364.

LIU Rong-hui, LU Li-yi, TAO Xuan, YU Hui, LI Hua. The Comparison of Genetic Polymorphism of SLA-DQA Gene Between Diannan Small-Ear Pig and Yunnan Wild Boar[J]. Laboratory Animal and Comparative Medicine, 2011, 31(5): 360-364.

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滇南小耳猪与云南野猪SLA-DQA基因的多态性比较

The Comparison of Genetic Polymorphism of SLA-DQA Gene Between Diannan Small-Ear Pig and Yunnan Wild Boar

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