内毒素致绵羊急性呼吸窘迫综合征多脏器的病理学改变

doi:10.3969/j.issn.1674-5817.2011.04.004

实验动物与比较医学 ›› 2011, Vol. 31 ›› Issue (4): 248-251.DOI: 10.3969/j.issn.1674-5817.2011.04.004

内毒素致绵羊急性呼吸窘迫综合征多脏器的病理学改变

牛廷献, 罗晓红, 万东君, 史智勇, 王红义, 陆璐

兰州军区兰州总医院动物实验科, 兰州 730050

收稿日期:2010-12-12 出版日期:2011-08-25 发布日期:2011-08-25
作者简介:牛廷献(1966-), 男, 副主任技师, 主要研究方向: 人类疾病动物模型的建立及相关研究。E-mail: niutx10000@163.com
基金资助:
甘肃省自然科学基金(No.1010RJZA052)

Pathological Changes of Multi-organ in Acute Respiratory Distress Syndrome Induced by Lipopolysaccharide in Sheep

NIU Ting-xian, LUO Xiao-hong, WAN Dong-jun, SHI Zhi-yong, WANG Hong-yi, LU Lu

Department of Animal Experiment, Lanzhou General Hospital, Lanzhou, 730050, China

Received:2010-12-12 Online:2011-08-25 Published:2011-08-25

摘要/Abstract

摘要： 目的观察急性呼吸窘迫综合征(ARDS)多脏器的病理学改变。方法静脉泵入小剂量内毒素(LPS)建立绵羊ARDS模型。72 h后处死,取脏器, 10%福尔马林固定,常规石蜡制片,HE染色,以光镜观察病理学改变。结果各脏器均有不同程度的病理学改变,以肺、肾最为严重,肝、脾和大肠次之,心脏较轻。并且以瘀血、变性、坏死为主要特征的病理学改变。结论 LPS诱导的ARDS可致多脏器发生不同程度的病理学改变,肺是最易受损伤的首位靶器官。

关键词: 急性呼吸窘迫综合征(ARDS), 绵羊, 内毒素(LPS), 病理学改变

Abstract: Objective To observe the pathological changes of multi-organ in acute respiratory distress syndrome(ARDS). Methods The sheep model of ARDS was established by infusing low dose lipopolysaccharide (LPS) intravenously. The sheep were sacrifised after 72 hours, the organs were collected and fixed by 10% formalin. Routine olefin cut sheets were made and stained by hematine-eosin. Then their pathology changes were observed under light microscope. Results Each organ has pathology change in different degree. The most severe organs of pathology change were lung and kidney, liver and spleen were secondary, but heart was not obvious. Moreover the key features of pathology change in organ were stagnant blood, degeneration and necrosis. Conclusion The multi-organ had pathology changes in different degree in ARDS by LPS inducement and lung was the first target organ that was the easiest impaired.

Key words: Acute Respiratory Distress Syndrome(ARDS), Sheep, Lipopolysaccharide (LPS), Pathological change

中图分类号:

Q95-33

牛廷献, 罗晓红, 万东君, 史智勇, 王红义, 陆璐. 内毒素致绵羊急性呼吸窘迫综合征多脏器的病理学改变[J]. 实验动物与比较医学, 2011, 31(4): 248-251.

NIU Ting-xian, LUO Xiao-hong, WAN Dong-jun, SHI Zhi-yong, WANG Hong-yi, LU Lu. Pathological Changes of Multi-organ in Acute Respiratory Distress Syndrome Induced by Lipopolysaccharide in Sheep[J]. Laboratory Animal and Comparative Medicine, 2011, 31(4): 248-251.

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内毒素致绵羊急性呼吸窘迫综合征多脏器的病理学改变

Pathological Changes of Multi-organ in Acute Respiratory Distress Syndrome Induced by Lipopolysaccharide in Sheep

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