SHIV1157ipd3N4小量多次阴道粘膜感染中国猕猴的初步研究

doi:10.3969/j.issn.1674-5817.2011.01.008

实验动物与比较医学 ›› 2011, Vol. 31 ›› Issue (1): 38-42.DOI: 10.3969/j.issn.1674-5817.2011.01.008

SHIV1157ipd3N4小量多次阴道粘膜感染中国猕猴的初步研究

丛喆¹, 蒋虹¹, 金光¹, 王卫¹, 高虹¹, 姚南¹, 陶真¹, 陈霆¹, 杨志伟¹, Sylvain.Fleury², AnickChalifour², Ruth.M.Ruprecht³, 魏强¹, 秦川¹

1.中国医学科学院医学实验动物研究所, 卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室, 北京 100021;
2.Dana-Farber Cancer Institute, Boston, Massachusetts 021151;Harvard Medical School, Boston, Massachusetts 021152;
3.Mymetics Corporation, CH 1066 Epalinges, Switzerland

收稿日期:2010-07-26 出版日期:2011-02-25 发布日期:2011-02-25
作者简介:丛喆, 硕士, 主管技师, 从事实验动物病毒分子生物学和模型研究工作。E-mail: zhengyihan@yahoo.com
基金资助:
国家十一五科技重大专项课题 (2009ZX10004- 402), 中央级公益性科研院所基本科研业务费专项资金(DWS 201009)

Efficient Repeated Low-dose Intravaginal Infection with SHIV1157ipd3N4 in Rhesus Monkey

CONG Zhe¹, JIANG Hong¹, JIN Guang¹, WANG Wei¹, GAO Hong¹, YAO Nan¹, TAO Zhen¹, CHEN Ting¹, YANG Zhi-wei¹, Sylvain. Fleury², Anick Chalifour², Ruth. M.Ruprecht³, WEI Qiang¹, QIN Chuan¹

1. Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Diseases Animal Models, State administration of Traditional Chinese medicine,Beijing 100021, China;
2. Dana-Farber Cancer Institute, Boston, Massachusetts 021151; Harvard Medical School,Boston, Massachusetts 021152;
3. Mymetics Corporation, CH 1066 Epalinges, Switzerland

Received:2010-07-26 Online:2011-02-25 Published:2011-02-25

摘要/Abstract

摘要： 目的建立R5-SHIV1157ipd3N4病毒中国猕猴阴道粘膜小剂量多次感染模型,为我国艾滋病疫苗有效性评价提供新的模型构建、应用策略。方法选用10-20TCID₅₀剂量的SHIV 1157ipd3N4病毒阴道黏膜途径感染6只雌性中国猕猴,共感染11次,每次攻毒间隔4~7 d。定期测定血浆病毒载量和外周血CD⁴⁺:CD⁸⁺。结果 6只中国猕猴经11次病毒攻击后,均建立系统性感染, 血浆病毒载量呈阳性; CD4+:CD8+均有下降。结论初步建立了R5-SHIV1157ipd3N4中国猕猴阴道黏膜小剂量多次感染模型,为艾滋病研究提供了更接近于自然感染状态的模型建立模式。

关键词: SHIV1157ipd3N4, 黏膜感染, 中国猕猴

Abstract: Objective To establish a CCR5-specific chimeric simian/human immunodeficiency virus, SHIV1157ipd3N4 repeated low-dose intravaginal infection model with rhesus monkeys. This new chimeric model could hopefully be more efficient model in AIDS vaccine development. Methods Six female Chinese rhesus monkeys were enrolled in this study. All animals were intra-vaginally challenged 11 times with 1 ml of a phosphate-buffer viral solution containing the heterologous SHIV1157ipd3N4, with 20 TCID₅₀ for the first 3 challenges and 10 TCID₅₀ for the remaining 8 challenges. Each challenge was accomplished at the interval of 4-7 days. Whole blood was collected and plasma virus was quantified by real-time SYBR Green RT-PCR and T cell subsets were determined by flow cytometry analysis. Results The systematic infection of six rhesus monkeys was established successfully after 11 challenges. The peaks of viral loads were between 10⁶copies/ml to 10⁸copies/ml.Conclusion The repeated low-dose technique was used for establishing a SHIV1157ipd3N4 infection model of Chinese rhesus monkey. This model aliking sexual route would be very useful for HIV-1 subtype C vaccine and pathogenesis studies.

Key words: SHIV1157ipd3N4, Vaginal transmission, Chinese rhesus monkey

中图分类号:

Q95-33

丛喆, 蒋虹, 金光, 王卫, 高虹, 姚南, 陶真, 陈霆, 杨志伟, Sylvain.Fleury, AnickChalifour, Ruth.M.Ruprecht, 魏强, 秦川. SHIV1157ipd3N4小量多次阴道粘膜感染中国猕猴的初步研究[J]. 实验动物与比较医学, 2011, 31(1): 38-42.

CONG Zhe, JIANG Hong, JIN Guang, WANG Wei, GAO Hong, YAO Nan, TAO Zhen, CHEN Ting, YANG Zhi-wei, Sylvain. Fleury, Anick Chalifour, Ruth. M.Ruprecht, WEI Qiang, QIN Chuan. Efficient Repeated Low-dose Intravaginal Infection with SHIV1157ipd3N4 in Rhesus Monkey[J]. Laboratory Animal and Comparative Medicine, 2011, 31(1): 38-42.

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SHIV1157ipd3N4小量多次阴道粘膜感染中国猕猴的初步研究

Efficient Repeated Low-dose Intravaginal Infection with SHIV1157ipd3N4 in Rhesus Monkey

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