The Effect of Six Kinds of Traditional Chinese Herbal Composing Prescriptions on Cell Proliferation and Melanogenesis in Cultured B16 Melanoma Cells

doi:10.3969/j.issn.1674-5817.2011.01.011

Abstract

Abstract: Objective To study the effects of six ethanol extracts and aqueous extracts from traditional Chinese herbal composing prescriptions on the cell proliferation and melanogenesis in cultured B16 melanoma cells. Methods The cell proliferation were measured by MTT method, and NaOH assay was to determine melanin synthesis. Results The aqueous extracts of composition 1,2,3 and alcohol extracts of composition 2,4,6 had significantly promoted cell proliferation (P<0.05); aqueous extract with the highest rate of cell proliferation was composing prescription 2 (0.625 mg/ml), the proliferation rate was 22.60%; ethanol extract with the highest rate of cell proliferation was composition 4 (0.078 mg/ml), the proliferation rate was 11.60%. The aqueous extract of composing prescriptions 1,2,4,5,6 and alcohol extracts of the six kinds of composing prescriptions promoted obviously the synthesis of melanin (P<0.05). Aqueous extract with the highest rate of melanin synthesis was composing prescription 1 (0.313 mg/ml), the growth rate is 75.38%; alcohol extract with the highest rate of melanin synthesis was composing prescription 1 (1.25 mg/ml), the growth rate was 88.01%. Conclusion The positive effect of each composing prescription on the proliferation of mice’s B16 melanoma cells and melanin synthesis is various due to the composing of the prescription.

Key words: Composing prescriptions, B16 melanoma cells, Cells proliferation, Melanogenesis

CLC Number:

Q95-33

ZHANG Jian, WU Wei, WAN Yu-hua, ZHANG Rong-wen, LIU Dan-dan, MA Rui, GU Wei-wang. The Effect of Six Kinds of Traditional Chinese Herbal Composing Prescriptions on Cell Proliferation and Melanogenesis in Cultured B16 Melanoma Cells[J]. Laboratory Animal and Comparative Medicine, 2011, 31(1): 52-56.

References

[1] Edward M,Gold JA,MacKie RM. Different suscep tibilities of melanoma cells to retinoic acid - induced changes in melanotic expression[J]. Biochem- Biophys- Res- Commun, 1988, 155(2):773-778.
[2] Matsuda H.Studies of cuticle drags from natural sources. IV.inhibitory effects of Arctostaphylos plants on melanin biosynthesis[J]. Bio Pharm Bull, 1996, 19(1):153.
[3] Hill SE, Buffey J, Thody AJ, et al.Investigation of the regulation of pigmentation in alpha - melanoyte - stimulating hormone responsive and unresponsive cultured B16 melanoma cells[J]. Pigment cell Res, 1989, 2(3):161-166.
[4] 涂彩霞, 张荣鑫, 刘亚玲, 等. 18味中药乙醇提取物对小鼠黑素瘤B16细胞增殖及黑素生成的影响[J]. 中华皮肤科杂志, 2006, 39(7):400-402.
[5] 吴品茹, 徐慧, 陈向东, 等. 熊果普和甘草黄酮对B16黑素瘤细胞株黑素合成的影响[J]. 组织工程与重建外科杂志,2008, 4(5):279-281.
[6] 姜泽群, 徐继敏, 吴琼, 等. 黑芝麻提取物促B16黑素瘤细胞黑素合成及其机制的研究[J]. 时珍国医国药, 2009, 20(9):2143-2145.
[7] 王辉, 王莒生, 王萍, 等. 中药滋补肝肾方对人黑素细胞株黑素合成的影响[J]. 中国中西医结合皮肤性病学杂志, 2004, 3(4):208-210.
[8] 王辉, 王莒生, 王萍, 等. 中药滋补肝肾方对鼠B16 黑素瘤细胞酪氨酸酶mRNA 表达的调节[J]. 中国皮肤性病学杂志, 2003, 17(4):230-231.
[9] 郁卫东, 杨立新, 刘桂生, 等. 滋补肝肾方激活小鼠B-16 黑色素瘤细胞线粒体ATP 合成酶-6 的基因表达[J]. 中国中西医结合杂志, 2004, 24:225-228.
[10] 刘雅男, 娄建石, 赵振宇, 等. 血清药理学方法研究复方中药对B16 黑素瘤细胞黑素合成的影响[J]. 中国中西医结合皮肤性病学杂志, 2005, 4(3):155-157.
[11] 薛文辉, 瞿幸, 陈长斌, 等. 中药消白灵汤对黑素细胞影响的实验研究[J]. 中国麻风皮肤病杂志, 2005, 21(8):613-615.
[12] 李永伟, 许爱娥, Ruth Halaban.加味桃红四物汤对黑素细胞酪氨酸酶和酪氨酸激酶受体基因蛋白表达的影响[J]. 中华皮肤科杂志, 2004, 37(12):727-729.
[13] 刘利平, 朱全红, 殷勇冠,等. 白癜风丸系列对酪氨酸酶活性影响的实验研究[J]. 中国麻风皮肤病杂志, 2005, 21(9):711-712.
[14] 刘之力, 李雅莉, 刘俐, 等. 六种中药复方乙醇提取物对酪氨酸酶激活作用及动物致色素作用的研究. 中国皮肤性病学杂志, 2005, 19(10):588-591.
[15] 邓燕, 刘祯, 肖艳, 等. 六味地黄方对体外培养的人正常黑素细胞增殖及黑素合成的影响[J].南方医科大学学报,2009, 29(4):701-703
[16] Masamoto Y, Iida S, Kubo M.Inhibitory effect of chinese crude drugs on tyrosinase[J].Plant Medica, 1980, 40(4):361.
[17] 雷铁池, 朱文元, 夏明玉, 等. 中药对黑素生物合成影响研究-82味中药乙醇提取物对酪氨酸酶活性的抑制作用[J].中草药, 1999, 30(5):336-339.