Nephrotoxicity of EDTA Utilized to Treat Chronic Cadmium Poisoning in Rats and Mice

doi:10.3969/j.issn.1674-5817.2015.01.012

Abstract

Abstract: Objective To investigate the side effects of (EDTA) utilized to treat chronic Cadmium poisoning in animals. Methods Male SD rats and NIH mice were randomly divided to the control group and model group respectively. The model group animals were daily given CdCl₂+ 2-Mercaptoethanol(ME, ip) for 5 days. 50 days or 30 days later, the rats or mice were randomly divided into model control group and EDTA treatment group respectively. The control group and model control group were injected with saline, and the EDTA group animals were treated with EDTA solution .At the end of the experiment, the urine, blood, kidneys and testis were collected for detecting the Cd concentration and pathologic changes. Results Compared with the model control group, in the EDTA treated groups (both in rats and mice), the Cd levels of kidney, urine and blood all increased significantly (P<0.05); protein precipitation could be detected in kidney tubules. Conclusion The EDTA could promote the accumulation of cadmium in the kidney. EDTA is not recommended to treat chronic cadmium poisoning patients.

Key words: Chronic cadmium poisoning, EDTA, Nephrotoxicity

CLC Number:

Q95-33

LI Guang-xian,ZHONG Zhi-yong,CHEN Jia-xing,et al. Nephrotoxicity of EDTA Utilized to Treat Chronic Cadmium Poisoning in Rats and Mice[J]. Laboratory Animal and Comparative Medicine, 2015, 35(1): 56-60. DOI: 10.3969/j.issn.1674-5817.2015.01.012.

References

[1] 环境保护部, 国土资源部. 全国土壤污染状况调查公报[R].2014-04-17.
[2] 乔实. 镉毒猛于虎-我国镉污染损害人体健康初探 [M]. 北京: 华夏出版社, 2013.
[3] Nawrot TS, Staessen JA, Roels HA.Cadmium exposure in the population: from health risks to strategies of prevention[J]. Biometals, 2010, 23(5):769-782.
[4] Sabath E, Robles-Osorio ML Renal health and the environment:heavy metal nephrotoxicity[J]. Nefrologia, 2012, 32(3):279-286.
[5] 王任群, 赵肃, 邱玉鹏, 等. 镉污染区居民肾功能损害的研究[J]. 环境与健康杂志, 2006, 5( 23):202-204.
[6] 李光先, 余日安. 镉毒性作用干预方法及其机制研究进展[J]. 中国职业医学, 2014, 41(2):222-226.
[7] 陈志强, 梁启荣. 依地酸二钠钙的驱镉效果及对肾脏功能的影响[J]. 医学文选, 2005, 4(3):339-340.
[8] 孙贵范. 职业卫生与职业医学[M]. 北京: 人民卫生出版社,2012.
[9] Flora SJ, Mittal M, Mehta A.Heavy metal induced oxidative stress & it’s possible reversal by chelation therapy[J]. Indian J Med Res, 2008, 128(4):501-523.
[10] 蔡婷峰, 董明, 高洪彬, 等. 镉致大鼠慢性肾损伤模型的探讨[J]. 中国职业医学, 2010, 37(3):194-198.
[11] 李颖超, 武昕, 严家荣, 等. 亚慢性镉中毒肾功能性损伤小鼠模型的研究[J]. 山西医科大学学报, 2013, 44(4):267-270.
[12] 中华人民共和国卫生行业标准. WS/T34-1996. 血中镉的石墨炉原子吸收光谱测定方法[S].
[13] 中华人民共和国卫生行业标准. WS/T32-1996. 尿中镉的石墨炉原子吸收光谱测定方法[S].
[14] Nordberg GF.Historical perspectives on cadmium toxicology[J]. Toxicol Appl Pharmacol, 2009, 238(3):192-200.
[15] Crinnion WJ.EDTA redistribution of lead and cadmium into the soft tissues in a human with a high lead burden-should DMSA always be used to follow EDTA in such cases[J].Altern Med Rev, 2011, 16(2):109-112.
[16] Gil HW, Kang EJ, Lee KH, et al.Effect of glutathione on the cadmium chelation of EDTA in a patient with cadmium intoxication[J]. Hum Exp Toxicol, 2011, 30(1):79-83.
[17] Buruko lu D, Baycu C. Protective effects of zinc on testes of cadmium-treated rats[J]. Bull Environ Contam Toxicol, 2008, 81(6):521-524.