Abstract: Objective To explore the dynamic changes of the glucose and insulin levels of obesity and diabetes cynomolgus monkey during 24 hours cycle. Methods Blood glucose and insulin levels from 36 adult male cynomolgus monkeys, which were uniformly selected from obesity, diabetes and normal group, were examined. Parameters including body weight, abdominal circumference, skin fold thickness, calculated body mass index (BMI), heart rate, blood pressure, breathing and body temperature were detected. Glucose, insulin, glycosylated hemoglobin and lipids levels were recorded by a interval of 1.5 hours in all individuals. Results The blood glucose value in normal, obesity and diabetes cynomolgus monkeys were 3.94±0.65 mmol/L, 4.33±0.84 mmol/L, and 12.09±4.58 mmol/L respectively. The blood glucose level of diabetes cynomolgus monkeys reached the peak during 21∶00-22∶30 with 13.68±0.55 mmol/L, and reached lowest point during 18:00-19:30 with 10.94±0.49 mmol/L. The insulin values in normal, obesity and diabetes cynomolgus monkeys were (29.50±12.36)-(62.50±21.98) mU/L, (31.21±0.98)-(123.42±3.38) mU/L, and (12.60±3.16)-(30.62±3.16) mU/L respectively. The maximum insulin value of diabetes monkeys was 30.62±11.71 mU/L during 9∶00~10∶30, and minimum of 12.60 ±3.16 mU/L during 03∶00~04∶30. Conclusion This study provides an immediate quantitative basis for investigating of glucose and insulin levels in cynomolgus monkeys, and it sets up a fundamental reference for primate models of diabetes or related diseases.

Key words: Obesity, Diabetes, Cynomolgus monkey, Blood glucose, Insulin