Dynamic Monitoring and Correlation Analysis of General Body Indicators, Blood Glucose, and Blood Lipid in Obese Cynomolgus Monkeys

doi:10.12300/j.issn.1674-5817.2024.091

Abstract

Abstract:

Objective This study aims to investigate the dynamic changes in general body parameters, blood glucose, and blood lipid profiles in obese cynomolgus monkeys, exploring the correlations among these parameters and providing a reference for research on the obese cynomolgus monkey model. Methods 30 normal male cynomolgus monkeys aged 5 - 17 years old (with body mass index < 35 kg/m2 and glycated hemoglobin content < 4.50%) and 99 spontaneously obese male cynomolgus monkeys (with body mass index ≥35 kg/m2 and glycated hemoglobin content < 4.50%) were selected. Over a period of three years, their abdominal circumference, skinfold thickness, body weight, body mass index, fasting blood glucose, glycated hemoglobin, and four blood lipid indicators were monitored. The correlations between each indicator were analyzed using repeated measurement ANOVA, simple linear regression, and multiple linear regression correlation analysis method. Results Compared to the control group, the obese group exhibited significantly higher levels of abdominal circumference, skinfold thickness, body weight, body mass index, and triglyceride (P＜0.05). In the control group, skinfold thickness increased annually, while other indicators remained stable. Compared with the first year, the obese group showed significantly increased abdominal circumference, skinfold thickness, body weight, body mass index, triglyceride, and fasting blood glucose in the second year(P＜0.05), with this increasing trend persisting in the third year (P＜0.05). In the control group, the obesity incidence rates in the second and third years were 16.67% and 23.33%, respectively, while the prevalence of diabetes remained at 16.67%. In the obese group, the diabetes incidence rates were 29.29% and 44.44% in years 2 and 3, respectively. Among the 11-13 year age group, the incidence rates were 36.36% and 44.68%, while for the group older than 13 years, the rates were 28.13% and 51.35%. Correlation analysis revealed significant associations (P＜0.05) between fasting blood glucose and age, abdominal circumference, skinfold thickness, body weight, and triglyceride in the diabetic monkeys. Conclusion Long-term obesity can lead to the increases in general physical indicators and fasting blood glucose levels in cynomolgus monkeys, and an increase in the incidence of diabetes. In diabetic cynomolgus monkeys caused by obesity, there is a high correlation between their fasting blood glucose and age, weight, abdominal circumference, skinfold thickness, and triglyceride levels, which is of some significance for predicting the occurrence of spontaneous diabetes.

Key words: Obesity, Cynomolgus monkey, Body indicator, Blood glucose, Blood lipid, Correlation analysis

CLC Number:

Q95-33

WEI Yanye,SHEN Guo,ZHANG Pengfei,et al. Dynamic Monitoring and Correlation Analysis of General Body Indicators, Blood Glucose, and Blood Lipid in Obese Cynomolgus Monkeys[J]. Laboratory Animal and Comparative Medicine, 2025, 45(1): 30-36. DOI: 10.12300/j.issn.1674-5817.2024.091.

Figures/Tables 4

References 17

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指标 Indicator	正常组（N=30） Normal group			肥胖组（N=99） Obese group			F值 F value			P值 P value
指标 Indicator	2022	2023	2024	2022	2023	2024	F_交互^a	F_时间^b	F_组间^c	P_交互^a	P_时间^b	P_组间^c
AC l/cm	37.12±4.27	38.13±5.14	36.93±6.42	46.58±7.35^**	50.03±8.47^**△△	49.77±8.53^**△△	3.487	5.969	67.589	0.032	<0.01	<0.01
SFT d/mm	4.55±2.21	8.30±4.43^△△	8.17±4.82^△△	13.40±9.29^**	18.76±10.14^**△△	22.01±12.82^**△△	3.524	20.057	39.045	0.032	<0.01	<0.01
BW m/kg	6.93±0.91	7.27±0.93	7.30±1.09	9.59±1.81^**	10.50±2.20^**△△	10.60±2.20^**△△	2.893	13.517	70.542	0.059	<0.01	<0.01
BMI	33.13±3.45	34.20±4.67	34.51±5.81	42.88±6.10^**	47.17±8.25^**△△	48.57±0.59^**△△	4.433	12.116	77.520	0.014	<0.01	<0.01
TG c/(mmol·L^-1)	0.63±0.23	0.77±0.34	0.69±0.23	0.75±0.45	1.08±0.67^*△△	0.98±0.49^**△△	1.968	9.659	8.423	0.144	<0.01	0.040
TC c/(mmol·L^-1)	1.86±0.41	2.26±0.42^△△	2.09±0.52	2.07±0.44^**	2.47±0.55^△△	2.10±0.45	4.480	53.847	2.704	0.012	<0.01	0.103
LDL-C c/(mmol·L^-1)	0.97±0.25	0.84±0.27	0.81±0.30	0.96±0.29	0.95±0.36	0.88±0.31^*	1.902	6.624	1.271	0.151	<0.01	0.262
HDL-C c/(mmol·L^-1)	1.69±0.60	2.23±0.69^△△	2.29±0.69^△△	2.07±0.67	2.26±0.77^△	2.06±0.64	7.541	12.433	0.302	0.010	<0.01	0.584
FPG c/(mmol·L^-1)	4.40±0.24	4.52±1.12	4.49±1.58	4.48±1.04	5.25±1.99^△△	5.10±1.83^△△	1.826	3.442	3.524	0.163	0.034	0.063
HbA1c ω/%	4.12±1.34	4.11±0.29	4.10±0.25	4.21±0.19	4.42±0.92^△	4.45±1.45	0.606	0.437	3.502	0.547	0.642	0.064

指标 Indicator	正常组（N=30） Normal group			肥胖组（N=99） Obese group			F值 F value			P值 P value
指标 Indicator	2022	2023	2024	2022	2023	2024	F_交互^a	F_时间^b	F_组间^c	P_交互^a	P_时间^b	P_组间^c
AC l/cm	37.12±4.27	38.13±5.14	36.93±6.42	46.58±7.35^**	50.03±8.47^**△△	49.77±8.53^**△△	3.487	5.969	67.589	0.032	<0.01	<0.01
SFT d/mm	4.55±2.21	8.30±4.43^△△	8.17±4.82^△△	13.40±9.29^**	18.76±10.14^**△△	22.01±12.82^**△△	3.524	20.057	39.045	0.032	<0.01	<0.01
BW m/kg	6.93±0.91	7.27±0.93	7.30±1.09	9.59±1.81^**	10.50±2.20^**△△	10.60±2.20^**△△	2.893	13.517	70.542	0.059	<0.01	<0.01
BMI	33.13±3.45	34.20±4.67	34.51±5.81	42.88±6.10^**	47.17±8.25^**△△	48.57±0.59^**△△	4.433	12.116	77.520	0.014	<0.01	<0.01
TG c/(mmol·L^-1)	0.63±0.23	0.77±0.34	0.69±0.23	0.75±0.45	1.08±0.67^*△△	0.98±0.49^**△△	1.968	9.659	8.423	0.144	<0.01	0.040
TC c/(mmol·L^-1)	1.86±0.41	2.26±0.42^△△	2.09±0.52	2.07±0.44^**	2.47±0.55^△△	2.10±0.45	4.480	53.847	2.704	0.012	<0.01	0.103
LDL-C c/(mmol·L^-1)	0.97±0.25	0.84±0.27	0.81±0.30	0.96±0.29	0.95±0.36	0.88±0.31^*	1.902	6.624	1.271	0.151	<0.01	0.262
HDL-C c/(mmol·L^-1)	1.69±0.60	2.23±0.69^△△	2.29±0.69^△△	2.07±0.67	2.26±0.77^△	2.06±0.64	7.541	12.433	0.302	0.010	<0.01	0.584
FPG c/(mmol·L^-1)	4.40±0.24	4.52±1.12	4.49±1.58	4.48±1.04	5.25±1.99^△△	5.10±1.83^△△	1.826	3.442	3.524	0.163	0.034	0.063
HbA1c ω/%	4.12±1.34	4.11±0.29	4.10±0.25	4.21±0.19	4.42±0.92^△	4.45±1.45	0.606	0.437	3.502	0.547	0.642	0.064

组别 Group	患病 Disease	年龄/岁 Age/ years old	发病数/只（发病率/%） Number of cases (Incidence rate / %)
组别 Group	患病 Disease	年龄/岁 Age/ years old	2023	2024
正常组（N=30） Normal group	肥胖Obese	<8	0 (0.00)	0 (0.00)
		8~10	1 (8.33)	2 (33.33)
		11~13	2 (25.00)	3 (23.08)
		>13	2 (22.22)	2 (20.00)
		总计	5 (16.67)	7 (23.33)
	糖尿病Diabetes mellitus	<8	0 (0.00)	0 (0.00)
		8~10	2 (16.67)	1 (16.67)
		11~13	2 (25.00)	3 (23.08)
		>13	1 (11.11)	1 (10.00)
		总计	5 (16.67)	5 (16.67)
肥胖组（N=99） Obese group	肥胖Obese	<8	5 (100.00)	2 (100.00)
		8~10	15 (83.33)	10 (90.91)
		11~13	44 (100.00)	44 (100.00)
		>13	32 (100.00)	34 (100.00)
		总计	96 (96.97)	90 (90.91)
	糖尿病Diabetes mellitus	<8	0 (0.00)	0 (0.00)
		8~10	4 (22.23)	4 (33.33)
		11~13	16 (36.36)	21 (44.68)
		>13	9 (28.13)	19 (51.35)
		总计	29 (29.29)	44 (44.44)
	高脂血症Hyperlipidemia	8~10	1 (5.56)	1 (8.33)
	高脂血症Hyperlipidemia	总计	1 (1.01)	1 (1.01)

组别 Group	患病 Disease	年龄/岁 Age/ years old	发病数/只（发病率/%） Number of cases (Incidence rate / %)
组别 Group	患病 Disease	年龄/岁 Age/ years old	2023	2024
正常组（N=30） Normal group	肥胖Obese	<8	0 (0.00)	0 (0.00)
		8~10	1 (8.33)	2 (33.33)
		11~13	2 (25.00)	3 (23.08)
		>13	2 (22.22)	2 (20.00)
		总计	5 (16.67)	7 (23.33)
	糖尿病Diabetes mellitus	<8	0 (0.00)	0 (0.00)
		8~10	2 (16.67)	1 (16.67)
		11~13	2 (25.00)	3 (23.08)
		>13	1 (11.11)	1 (10.00)
		总计	5 (16.67)	5 (16.67)
肥胖组（N=99） Obese group	肥胖Obese	<8	5 (100.00)	2 (100.00)
		8~10	15 (83.33)	10 (90.91)
		11~13	44 (100.00)	44 (100.00)
		>13	32 (100.00)	34 (100.00)
		总计	96 (96.97)	90 (90.91)
	糖尿病Diabetes mellitus	<8	0 (0.00)	0 (0.00)
		8~10	4 (22.23)	4 (33.33)
		11~13	16 (36.36)	21 (44.68)
		>13	9 (28.13)	19 (51.35)
		总计	29 (29.29)	44 (44.44)
	高脂血症Hyperlipidemia	8~10	1 (5.56)	1 (8.33)
	高脂血症Hyperlipidemia	总计	1 (1.01)	1 (1.01)

指标 Indicator	年龄 Age	FPG	HbA1c	BW	AC	SFT	BMI	TG	TC	LDL-C	HDL-C
年龄Age	-	0.548^**	0.339	0.090	0.291	0.218	0.093	0.530^**	0.281	0.224	-0.079
FPG		-	0.819^**	0.492^*	0.481^*	0.338	0.463^*	0.565^**	-0.017	-0.003	-0.313
HbA1c			-	0.388	0.322	0.383	0.364	0.772^**	0.077	0.025	-0.359
BW				-	0.907^**	0.774^**	0.944^**	0.151	-0.427^*	-0.117	-0.458^*
AC					-	0.838^**	0.870^**	0.238	-0.386	-0.123	-0.472^*
SFT						-	0.745^**	0.389	-0.374	-0.222	-0.527^**
BMI							-	0.112	-0.418^*	-0.057	-0.461^*
TG								-	0.169	-0.002	-0.395
TC									-	0.713^**	0.681^**
LDL-C										-	0.291
HDL-C											-