Expression and Distribution of Molecules Regulating Protein Degradation Pathway in Nervous System of Mice

doi:10.3969/j.issn.1674-5817.2020.06.001

Abstract

Abstract: Objective To investigate the expression and distribution of the molecules regulating protein degradation pathway in the nervous system of mice. Methods The eye, olfactory bulb, cortex, hippocampus, striatum, midbrain, cerebellum, brainstem, spinal cord, sciatic nerve and muscle of 6 weeks wild-type C57BL/6J mice were collected. Then the distribution and expression of key proteins in protein degradation pathway in different tissues, anatomical sites and subcellular structures were determined by Western blotting. Results Cathepsin D, lysosomal-associated membrane protein 2 (LAMP2), microtubule-associated protein 1A/1B-light chain 3 (LC3A/B), autophagy-related protein (Atg3), Ras-related GTP-binding protein7 (Rab7), Calpain and Calpastatin had different expression levels in the eye, brain, spinal cord, sciatic nerve and muscle. Compared to the spinal cord, the sciatic nerve showed decreased levels of ubiquitin, Cathepsin D, LAMP2, total LC3A/B, Atg3, Rab7 and full length of Calpain1 large subunit (P<0.05), and increased level of Calpain small subunit (P<0.05). Conclusion The ubiquitin-proteasome system and autophagy mainly are widespread in the cell under physiological conditions, which mainly occurs in the cell body of neurons; but the level of spliced Calpain small in axon is relatively high, which suggests that Calpain degradation pathway may play a major role in axon.

Key words: Ubiquitin-proteasome system, Autophagy, Calpain, Spinal cord, Sciatic nerve, Mice

CLC Number:

SONG Binbin, DONG Wenzhou, JIA Bingquan, ZHEN Ran, PENG Yu, YANG Xuan, YU Jia. Expression and Distribution of Molecules Regulating Protein Degradation Pathway in Nervous System of Mice[J]. Laboratory Animal and Comparative Medicine, 2020, 40(6): 463-469.

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