Abstract: Objective To learn and analyze the genetic quality of C57BL/6J mouse so as to provide some reference for laboratory animal genetic quality control and management for production units and for the utilization of C57BL/6J. Methods Forty two microsatellite loci which were optimized previously and distribute 17 autosomes and X chromosome were selected in present study. PCR and STR scanning by these 42 loci were performed to detect the genetic quality of 5 main domestic C57BL/6J mouse populations from Beijing, Shanghai and Nanjing. Results The results of STR scanning showed that 42 microsatellite loci demonstrated monomorphism in C57BL/6J populations from Shanghai and Nanjing. However, populations from Beijing displayed polymorphism. Thereinto, Animal number 3 from Jing 1 population presented polymorphism at locus D15Mit105 compared to other 9 mice and other 4 populations. Meanwhile all the mice in Jing1 population at locus D10Mit3 show polymorphism compared with other 4 populations. Animal number 8 at locus D14Nds1 and animal number 6 at locus D2Nds3 showed polymorphism within Jing 2 population. Animal number 1 from Jing 3 population also showed polymorphism within population. Conclusions C57BL/6J mouse from Shanghai and Nanjing showed genetic consistency. However, genetic differences existed in 3 production populations in Beijing. These results should be paid much more attention by producers and users.

Key words: C57BL/6J mouse, Microsatellite, Polymorphism, Genetic quality