Analysis on Genetic Diversity of Macaca mulatta littoralis by Using Microsatellite DNA Markers

doi:10.3969/j.issn.1674-5817.2017.06.003

Abstract

Abstract: Objective To analyze the genetic diversity of Macaca mulatta littoralis and providing basic data for genetic quality monitoring methods of the population. Methods Twenty-eight Macaca mulatta littoralis were genotyped by using 20 microsatellite DNA markers, DNA was extracted from blood and amplified by PCR, and PCR products were analyzed by non-modified polyacrylamide gel electrophoresis. Every microsatellite locus was calculated by software POPGENE 1.32 and Excel. Results All 20 microsatellite loci were highly polymorphic, 200 alleles were found in the population.The number of alleles (Na), effective number of alleles (Ne), expected heterozygosity (He), observed heterozygosity (Ho), Shannon's information index (I) and polymorphism information content (PIC) were ranged from 5 to 16, 3.7333 to 11.6148, 0.7455 to 0.9305, 0.3214 to 0.7500, 1.3998 to 2.5963, and 0.6862 to 0.9074, with an average of 10, 6.8438, 0.8509, 0.4607, 2.0166 and 0.8154, respectively. All of 17 loci showed significant deviations from Hardy-Weinberg equilibrium. Conclusions There are high genetic diversity in Macaca mulatta littoralis of this colony. The experiments can provide a theoretical foundation for establishing genetic quality monitoring method with specific microsatellite loci.

Key words: Microsatellite marker, Macaca mulatta littoralis, Genetic diversity

CLC Number:

Q95-33

ZHOU Jian-hua, LI Zhi-xiong, YANG Yan-yan, XIE Jin-dong, YU Chun-ying, WANG Xun-li. Analysis on Genetic Diversity of Macaca mulatta littoralis by Using Microsatellite DNA Markers[J]. Laboratory Animal and Comparative Medicine, 2017, 37(6): 434-441.

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