Protective Effect of Naoxintong Capsule on Brain Cell Injury in Rats with Ketamine Addiction

doi:10.3969/j.issn.1674-5817.2019.06.003

Abstract

Abstract: Objective To investigate the protective effect of naoxintong capsule on brain cell injury in rats with ketamine addiction. Methods Sixty SD rats were randomly divided into control, model, Naoxintong capsule with high, medium and low dose, and positive control group, with 10 rats in each group. The model group, Naoxintong capsule high, medium and low dose group and positive control group was intraperitoneally injected with 50 mg/mL ketamine hydrochloride injection (10 mg/kg). Naoxintong Capsule high, medium and low dose group was gavaged with Naoxintong Capsule (500 mg/kg, 250 mg/kg and 100 mg/kg). Positive control group was intraperitoneally injected with L-tetrahydropalmatine (20 mg/kg). Control group was intraperitoneally injected with same amount of normal saline, once a day for 7 days. The conditional position preference (CPP) of each rat was detected before and after the experiment. After experiment, expression of brain tissue c-fos gene was detected by immunohistochemistry. The expression of B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax) and cysteinyl aspartate specific proteinase-3 (Caspase-3) protein in brain tissue was determined by Western blotting. HE staining was performed to observe pathological changes of brain tissue lesions. Results Compared with the control group, the CPP of the model group, Naoxintong capsule high, medium and low dose group and positive control group were significantly increased (P<0.05), c-fos gene, expressions of Caspase-3 and Bax protein in brain tissue were significantly increased (P<0.05), Bcl-2 expression was significantly decreased (P<0.05). Compared with the model group, CPP of Naoxintong capsule high and medium dose group and positive control group was significantly decreased (P<0.05), c-fos gene, expression of Caspase-3 and Bax protein were significantly decreased (P<0.05), and Bcl-2 expression was significantly increased (P<0.05). HE staining showed that degree of brain cell lesions was reduced. Conclusion Naoxintong Capsule may protect brain cells injury of rats with ketamine addiction by regulating apoptosis of brain tissues.

Key words: Naoxintong capsule, Ketamine, Rat, Brain cell injury

CLC Number:

DF795.1Q95-33

LIU Xiao-bo, YANG Mei, Song Jia. Protective Effect of Naoxintong Capsule on Brain Cell Injury in Rats with Ketamine Addiction[J]. Laboratory Animal and Comparative Medicine, 2019, 39(6): 437-442.

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