Establishment of Non-exposed Tracheal Instillation Method in Rats under Head Lamp

doi:10.3969/j.issn.1674-5817.2019.05.014

Abstract

Abstract: Objective To explore a new method for the operation of a rat model of non- exposed tracheal instillation. Methods Tracheal intubation with a self made disposable intravenous indwelling needle in the irradiation under a strong light, 0.1 mL saline was administered repetitive operation to 10 SD male rats (200-350 g) by 1 mL syringe with the frequency of once per two days. Results A total of 40 operations were performed, each animal took about 3 minutes. The whole experiment was safe and smooth, and the animals were not injured. During the experiment and the end of the experiment,animals had no abnormal reaction after three days. Conclusion This method is rapid, safe and effective, it can be widely used in the experimental study of respiratory tract administration in rats.

Key words: Rat, Intratracheal instillation, Experimental technique

CLC Number:

Q95-33

LONG Long, TAN Hong, YANG Hong-bo. Establishment of Non-exposed Tracheal Instillation Method in Rats under Head Lamp[J]. Laboratory Animal and Comparative Medicine, 2019, 39(5): 408-410.

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Establishment of Non-exposed Tracheal Instillation Method in Rats under Head Lamp

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