Preliminary Construction and Analysis of Right Ventricular Overload Model in Newborn Rabbits

doi:10.3969/j.issn.1674-5817.2019.03.003

Abstract

Abstract: Objective To construct a neonatal rabbit model of right ventricular overload for studying the pathophysiological remodeling of right ventricle in congenital heart disease patients with right ventricular overload. Methods Thirty neonatal rabbits were selected and randomly divided into two groups. According to different surgical procedures, they were divided into surgery of pulmonary artery banding (PAB) group and sham surgery group, each group with 15 rabbits. The surgical methods of the PAB group were as follows: horizontal thoracotomy was performed through dissecting intercostal muscles and splitting the sternum along the intercostal space. Pulmonary artery was banded by a 6-0 silk thread. At 14 days after surgery, the constriction of pulmonary artery was confirmed by echocardiography. The right ventricular systolic and diastolic pressures were measured by cardiac catheterization. The histological changes of the hearts from sham and PAB group were evaluated after Hematoxylin and Eosin staining. Results Right ventricular overload was successfully established by constriction of the pulmonary artery in neonatal rabbits with the survival rate 73.33% at 14 days after surgery. Relative to a sham operation group, the peak pressure gradient across pulmonary artery constriction, right ventricular systolic and diastolic pressures, and free wall thickness were significantly increased in PAB group at 14 days after surgery (P<0.01). Conclusion The right ventricular overload model was successfully established through pulmonary arterybanding in neonatal rabbits, which may be used for studying the pathophysio-logical changes of congenital heart disease with right ventricular overload.

Key words: Pulmonary artery banding(PAB), Neonatal Rabbit, Right ventricular remodeling

CLC Number:

Q95-33

DING Lei, WANG Shou-bao, JING Hui, LI Yao. Preliminary Construction and Analysis of Right Ventricular Overload Model in Newborn Rabbits[J]. Laboratory Animal and Comparative Medicine, 2019, 39(3): 187-192.

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