Establishment of Two Kinds of Lung Cancer Models in Mouse and Evaluation by Micro PET-CT

doi:10.3969/j.issn.1674-5817.2019.01.008

Abstract

Abstract: Objective To compare the difference of two kinds of lung cancer models in mouse, which were established by injecting with the human non-small cell lung cancer (NSCLC) cells through tail vein or orthotopically inoculating in the lung using a microsyringe through the intercostals space. Methods The NCI-H1975 cells of NSCLC were injected into the NOD-SCID mouse via tail vein or intercostals space. After 15 days, mice were scanned by micro PET-CT to monitor the tumor growth and metastasis. Then mice were sacrificed and their lungs were dissected, fixed with phosphate-buffered neutral formalin and prepared for standard histological examination. Results Mice that were injected with the NCI-H1975 cells through tail vein for 15 days did not form tumor nodules on the lung. However, the mice that were orthotopically inoculated in the lung through intercostals space significantly had tumor nodules in their lungs. Conclusion A NOD-SCID mouse lung tumor model can be successfully established by transplanting NCI-H1975 cells into the lung through intercostals space. This model is simple, repeatable, and efficient.

Key words: Mouse orthotopic lung cancer model, Micro PET-CT, Tail intravenous injection

CLC Number:

Q95-33

SHEN Yan,WANG Yun,ZHANG Ru,et al. Establishment of Two Kinds of Lung Cancer Models in Mouse and Evaluation by Micro PET-CT[J]. Laboratory Animal and Comparative Medicine, 2019, 39(1): 39-45. DOI: 10.3969/j.issn.1674-5817.2019.01.008.

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