Laboratory Animal and Comparative Medicine ›› 2019, Vol. 39 ›› Issue (1): 26-33.DOI: 10.3969/j.issn.1674-5817.2019.01.006

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The Silence Effect of siRNA Sequences Targeting on Vegf/Vegfr Genes in Rat Endothelial Cells

CHEN Lin-zhong-ri1, YIN Dong-lai1, ZHAO Ze-ting1, WEI Qian-han1, CHEN Xin1, ZHANG Yu-mei1,2   

  1. 1. Institute of Comparative Medicine, Yangzhou University, Yangzhou, Jiangsu, 225009, China;
    2. Jiangsu Coinnovation Center for Prevention and Control of Important AnimalInfectious Diseases and Zoonoses, Yangzhou, Jiangsu, 225009, China
  • Received:2018-09-12 Online:2019-02-25 Published:2021-01-29

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Abstract: Objective The effective siRNA sequences targeting on Vegf/Vegfr were screened for interference the expression of Vegf and Vegfr genes. Methods In this study, three siRNA sequences each gene were designed based on the gene of rat Vegf, Vegfr1 (Flt1) and Vegfr2 (Flk1). The effects of gene silence of these siRNA nucleotides on its target gene were evaluated by Western Blot and RT-PCR after transfection in rat endothelial cells. Result VEGF can be silenced by two siRNA sequences target on Vegf gene, FLT1 and FLK1 can be effectively depressed by one sequence target on Flt1 and Flk1 genes respectively. Conclusion The effective sequences were screened and could be used in angiogenesis research of VEGF/VEGFR signaling.

Key words: Vascular Endothelial Growth Factor (VEGF), VEGF receptor(VEGFR), siRNA, Rat

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