Abstract: Objective To investigate the domestic situation of intranasal administration in mouse. Method The data was retrieved from Wanfang database in the search term “mouse” and “intranasal administration” as defined by the 2011 to 2017 Journal Citation Reports. Perform a statistical analysis on the articles which meet the criterion. Result 770 articles were retrieved, and 513 articles described the dose of intranasal administration. In the 513 articles, there are 272 articles which the dose of intranasal administration is between 30 µL to 50 µL, and the ratio is 53.3%. The ratio of the articles that did not describe whether the personals use anesthetics or not in intranasal administration is 49.2%. The most used three anesthetics are diethyl ether (51%), barbiturates (24%) and chloral hydrate (12%) in the 249 articles which described the anesthetics used in intranasal administration. Conclusion Except the especial experiments, the dose of intranasal administration should be limited in 50 µL and the mouse should be anesthetized. The most used anesthetics in intranasal administration is diethyl ether. As diethyl ether is inflammable and explosive, we suggest it should be replaced by isoflurane in intranasal administration.

Key words: Mice, Intranasal administration, Dose, Anesthesia